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1.发明申请METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLERECOGNITION SITES 审中-公开使用多重分解位点合成核酸分子的方法和组合物公开(公告)号：US20130004996A1
公开(公告)日：2013-01-03
申请号：US13219440
申请日：2011-08-26
申请人： Jonathan D. CHESNUT , John Carrino , Louis Leong , Knut Madden , Martin A. G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R.N. Byrd , Gary F. Temple
发明人： Jonathan D. CHESNUT , John Carrino , Louis Leong , Knut Madden , Martin A. G. Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R.N. Byrd , Gary F. Temple
CPC分类号： C12N15/66 , C12N9/90 , C12N15/10 , C12N15/64 , C12N15/902 , C12N2800/108 , C12N2800/30 , C12N2800/70 , C12N2840/20 , C12P19/34 , C12Y599/01
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according tb the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
摘要翻译： 本发明提供了用于重组克隆的组合物和方法。 组合物包括具有多个重组位点和/或多个拓扑异构酶识别位点的载体。 该方法允许同时克隆两种或更多种不同的核酸分子。 在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。 本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。 本发明还提供包含本发明的核酸分子或根据本发明的方法制备的宿主细胞，并且还提供了包含本发明的组合物，宿主细胞和核酸分子的试剂盒，其可用于合成核酸分子 根据本发明的方法。
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2.发明申请公开(公告)号：US20100267120A1
公开(公告)日：2010-10-21
申请号：US12497915
申请日：2009-07-06
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
摘要翻译： 通过在体外和体内使用核酸，载体和方法，使用工程化重组位点和重组蛋白来移动或交换DNA分子片段来提供重组克隆，以提供具有所需特征的嵌合DNA分子和 /或DNA片段。
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公开(公告)号：US20090186386A1
公开(公告)日：2009-07-23
申请号：US12233836
申请日：2008-09-19
IPC分类号： C12P19/34
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
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公开(公告)号：US20090149640A1
公开(公告)日：2009-06-11
申请号：US12268269
申请日：2008-11-10
申请人： A-Li W. Hu , James L. Hartley , Heather J. Jordan
发明人： A-Li W. Hu , James L. Hartley , Heather J. Jordan
CPC分类号： C07H21/00 , C12N15/10 , C12Q1/68 , C12Q1/6827 , C12Q1/6876
摘要： The present invention provides nucleic acid molecules which may be used as standards for estimating the size (in base pairs) and mass of linear, double-stranded or single-stranded nucleic acid molecules separated by size. The nucleic acid molecules of the invention may be DNA molecules, RNA molecules or DNA/RNA hybrid molecules, and may be double-stranded or single-stranded. The invention also provides methods for producing nucleic acid sizing ladders from these nucleic acid molecules, ladders produced by such methods, and methods for estimating the size and mass of nucleic acid molecules by comparison to these nucleic acid sizing ladders.
摘要翻译： 本发明提供核酸分子，其可以用作估计由大小分开的线性，双链或单链核酸分子的大小（碱基对）和质量的标准。 本发明的核酸分子可以是DNA分子，RNA分子或DNA / RNA杂交分子，并且可以是双链或单链的。 本发明还提供了用于从这些核酸分子生产核酸筛选梯级的方法，通过这些方法产生的梯子，以及通过与这些核酸分级梯相比较估计核酸分子的大小和质量的方法。
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公开(公告)号：US20090111112A1
公开(公告)日：2009-04-30
申请号：US12237587
申请日：2008-09-25
申请人： James L. HARTLEY
发明人： James L. HARTLEY
IPC分类号： C12Q1/68
摘要： The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
摘要翻译： 本发明涉及作为限制性内切核酸酶消化物的核酸标记梯，其中核酸限制性内切核酸酶消化物是由一种或多种限制性内切酶完全消化一种或多种核酸而产生的核酸片段的集合; 限制性内切酶消化物包含至少3个片段; 并且碱基对中的片段的大小是整数的倍数，其中整数为10以上。
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公开(公告)号：US07455974B2
公开(公告)日：2008-11-25
申请号：US11534575
申请日：2006-09-22
申请人： James L. Hartley
发明人： James L. Hartley
摘要： The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
摘要翻译： 本发明涉及作为限制性内切核酸酶消化物的核酸标记梯，其中核酸限制性内切核酸酶消化物是由一种或多种限制性内切核酸酶完全消化一种或多种核酸而产生的核酸片段的集合; 限制性内切酶消化物包含至少3个片段; 并且碱基对中的片段的大小是整数的倍数，其中整数为10以上。
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公开(公告)号：US07282326B2
公开(公告)日：2007-10-16
申请号：US10058292
申请日：2002-01-30
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
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8.发明授权公开(公告)号：US07244560B2
公开(公告)日：2007-07-17
申请号：US10005876
申请日：2001-12-07
申请人： Jonathan D. Chestnut , John Carrino , Louis Leong , Knut Madden , Martin Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R.N. Byrd , Gary F. Temple
发明人： Jonathan D. Chestnut , John Carrino , Louis Leong , Knut Madden , Martin Gleeson , James Fan , Michael A. Brasch , David Cheo , James L. Hartley , Devon R.N. Byrd , Gary F. Temple
IPC分类号： C12Q1/68
摘要： The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
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公开(公告)号：US06270969B1
公开(公告)日：2001-08-07
申请号：US09233492
申请日：1999-01-20
IPC分类号： C12Q168
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
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公开(公告)号：US06171861B2
公开(公告)日：2001-01-09
申请号：US09005476
申请日：1998-01-12
IPC分类号： C12N1563
摘要： Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
摘要翻译： 通过在体外和体内使用核酸，载体和方法，使用工程化重组位点和重组蛋白来移动或交换DNA分子片段来提供重组克隆，以提供具有所需特征的嵌合DNA分子和 /或DNA片段。
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