公开(公告)号：US5115101A

公开(公告)日：1992-05-19

申请号：US721005

申请日：1991-06-26

申请人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

发明人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

IPC分类号： C07K16/06 ,  C07K16/36 ,  G01N33/68

CPC分类号： G01N33/6842 ,  C07K16/065 ,  C07K16/36 ,  G01N33/68

摘要： Antibody preparation purified using immobilized protein A and yet substantially free of protein A that may have solubilized during the purificaiton process. The antibodies include less than 15 ng protein A per mg of antibody, preferably less than 1 ng/mg. Low protein A content is obtained by first contacting the antibodies and solubilized protein A with an ion exchange resin under conditions to adsorb both. The antibodies and protein A are then sequentially eluted under conditions of increasing ionic strength.

摘要翻译： 使用固定化蛋白A纯化但基本上不含可能在纯化过程中溶解的蛋白A的抗体制备。 抗体包括每mg抗体少于15ng蛋白A，优选小于1ng / mg。 通过在吸附两者的条件下首先使抗体和溶解的蛋白A与离子交换树脂接触来获得低蛋白质A含量。 然后在增加离子强度的条件下将抗体和蛋白A顺序洗脱。

公开(公告)号：US4983722A

公开(公告)日：1991-01-08

申请号：US204054

申请日：1988-06-08

申请人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

发明人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

IPC分类号： G01N33/53 ,  A61K39/395 ,  C07K1/18 ,  C07K14/435 ,  C07K14/705 ,  C07K16/00 ,  C07K16/06 ,  C07K16/36 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  G01N33/531 ,  G01N33/68

CPC分类号： G01N33/6842 ,  C07K16/065 ,  C07K16/36 ,  G01N33/68

摘要： Protein A is selectively isolated from an antibody--Protein A mixture by exposing the mixture to an anion exchange material under conditions sufficient to adsorb both components and then sequentially eluting the antibodies and protein A under conditions of increasing ionic strength. Resulting antibody preparations have less than about 15 ng of Protein A per mg of antibody.

公开(公告)号：US4478825A

公开(公告)日：1984-10-23

申请号：US542243

申请日：1983-10-14

申请人： James W. Bloom

发明人： James W. Bloom

IPC分类号： A61K38/00 ,  C07K14/755 ,  A61K35/16

CPC分类号： C07K14/755 ,  A61K38/00

摘要： Antihemophilic factor (AHF), a blood protein component necessary for clotting of normal whole blood, is extracted and concentrated from a cryoprecipitate suspension containing AHF and fibrinogen as its principal components by: selectively precipitating fibrinogen from a water-ethanol solution and removing the ethanol therefrom.

摘要翻译： 通过以下方法从含有AHF和纤维蛋白原的冷沉淀物悬浮液中提取并浓缩来自正常全血凝血所必需的血液蛋白成分的抗血友病因子（AHF）：从水 - 乙醇溶液中选择性沉淀纤维蛋白原，并从其中除去乙醇 。