公开(公告)号：US5115101A

公开(公告)日：1992-05-19

申请号：US721005

申请日：1991-06-26

申请人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

发明人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

IPC分类号： C07K16/06 ,  C07K16/36 ,  G01N33/68

CPC分类号： G01N33/6842 ,  C07K16/065 ,  C07K16/36 ,  G01N33/68

摘要： Antibody preparation purified using immobilized protein A and yet substantially free of protein A that may have solubilized during the purificaiton process. The antibodies include less than 15 ng protein A per mg of antibody, preferably less than 1 ng/mg. Low protein A content is obtained by first contacting the antibodies and solubilized protein A with an ion exchange resin under conditions to adsorb both. The antibodies and protein A are then sequentially eluted under conditions of increasing ionic strength.

摘要翻译： 使用固定化蛋白A纯化但基本上不含可能在纯化过程中溶解的蛋白A的抗体制备。 抗体包括每mg抗体少于15ng蛋白A，优选小于1ng / mg。 通过在吸附两者的条件下首先使抗体和溶解的蛋白A与离子交换树脂接触来获得低蛋白质A含量。 然后在增加离子强度的条件下将抗体和蛋白A顺序洗脱。

公开(公告)号：US4983722A

公开(公告)日：1991-01-08

申请号：US204054

申请日：1988-06-08

申请人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

发明人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

IPC分类号： G01N33/53 ,  A61K39/395 ,  C07K1/18 ,  C07K14/435 ,  C07K14/705 ,  C07K16/00 ,  C07K16/06 ,  C07K16/36 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  G01N33/531 ,  G01N33/68

CPC分类号： G01N33/6842 ,  C07K16/065 ,  C07K16/36 ,  G01N33/68

摘要： Protein A is selectively isolated from an antibody--Protein A mixture by exposing the mixture to an anion exchange material under conditions sufficient to adsorb both components and then sequentially eluting the antibodies and protein A under conditions of increasing ionic strength. Resulting antibody preparations have less than about 15 ng of Protein A per mg of antibody.