公开(公告)号：US07282180B2

公开(公告)日：2007-10-16

申请号：US10612144

申请日：2003-07-02

申请人： Arjan Tibbe ,  Jan Greve ,  Leon W. M. M. Terstappen ,  Jan Greve, legal representative

发明人： Bart De Grooth, deceased

IPC分类号： G01N21/00

CPC分类号： G01N33/54326

摘要： Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to automated laser scanning to generate sequential digitized x-y sub-images or partial images of target and non-target objects that are combined to form reconstructed full images, thereby allowing detection, enumeration, differentiation and characterization of imaged objects on the basis of size, morphology and immunophenotype.

摘要翻译： 公开了用于自动收集和图像分析的装置和方法，其能够识别或分类对准或沉积在表面上的微观物体。 这些物体，例如。 可检测标记的稀有目标细胞是磁性或非磁性固定的，并进行自动激光扫描以产生被组合以形成重建的全部图像的目标和非目标对象的顺序数字化的xy子图像或部分图像，从而允许检测， 基于大小，形态和免疫表型的成像物体的计数，分化和表征。