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公开(公告)号：US06110678A
公开(公告)日：2000-08-29
申请号：US70998
申请日：1998-05-01
CPC分类号： C12Q1/6834 , C12Q1/6813
摘要： A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions, which preferably differ in temperature only, is disclosed. The two hybridization conditions control the order of hybridization, where the first hybridization conditions allow hybridization of the capture probe to the target polynucleotide, and the second hybridization conditions allow hybridization of the capture probe to the immobilized probe. The method may be used to detect the presence of a target polynucleotide in a sample by detecting the captured target polynucleotide or amplified target polynucleotide.
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公开(公告)号：US06534273B2
公开(公告)日：2003-03-18
申请号：US09956412
申请日：2001-09-18
申请人： William G. Weisburg , Jay H. Shaw , Michael M. Becker , Mehrdad R. Majlessi , Steven T. Brentano , Kiyotada Nunomura
发明人： William G. Weisburg , Jay H. Shaw , Michael M. Becker , Mehrdad R. Majlessi , Steven T. Brentano , Kiyotada Nunomura
IPC分类号： C12Q168
CPC分类号： C12Q1/6834 , C12Q1/6813
摘要： A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions that control the order of hybridization, where the first hybridization condition allows hybridization of the capture probe to the target polynucleotide, and the second hybridization condition allows hybridization of the capture probe to the immobilized probe. The method further includes amplifying the captured target polynucleotide by hybridizing at least one primer oligonucleotide to the target polynucleotide and using nucleic acid amplification that initiates from the primer oligonucleotide.
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公开(公告)号：US06280952B1
公开(公告)日：2001-08-28
申请号：US09574561
申请日：2000-05-19
IPC分类号： C12Q168
CPC分类号： C12Q1/6834 , C12Q1/6813
摘要： A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions, which preferably differ in temperature only, is disclosed. The two hybridization conditions control the order of hybridization, where the first hybridization conditions allow hybridization of the capture probe to the target polynucleotide, and the second hybridization conditions allow hybridization of the capture probe to the immobilized probe. The method may be used to detect the presence of a target polynucleotide in a sample by detecting the captured target polynucleotide or amplified target polynucleotide.
摘要翻译： 公开了一种通过使用捕获探针和两种不同的杂交条件（其优选温度不同）将样品中的靶多核苷酸捕获到具有附着的固定化探针的固体支持物上的方法。 两个杂交条件控制杂交的顺序，其中第一杂交条件允许捕获探针与靶多核苷酸杂交，第二杂交条件允许捕获探针与固定的探针杂交。 该方法可以通过检测捕获的靶多核苷酸或扩增的靶多核苷酸来检测样品中靶多核苷酸的存在。
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