公开(公告)号：US07635471B2

公开(公告)日：2009-12-22

申请号：US11506891

申请日：2006-08-21

申请人： Kou-Joan Cheng ,  Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Shiou Hua Lin ,  Je Ruei Liu ,  Bi Yu

发明人： Kou-Joan Cheng ,  Yo-Chia Chen ,  Hsueh-Ling Cheng ,  Shiou Hua Lin ,  Je Ruei Liu ,  Bi Yu

IPC分类号： A61K38/47 ,  A61K38/54 ,  C12N9/42 ,  C12N9/28 ,  C12N9/36 ,  C07H21/04

CPC分类号： C12N9/2434 ,  A61K38/47

摘要： A xylanase gene, denoted xynR8, encoding a xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermostable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the β-1,4-glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.

摘要翻译： 提供了一种称为xynR8的木聚糖酶基因，其编码从单分解的瘤胃微生物获得的木聚糖酶（XynR8）。 还提供了xynR8基因，木聚糖酶的DNA序列，该酶是热稳定的，并且对具有高活性的木聚糖具有高度特异性。 描述了具有xynR8基因的微生物宿主的转化。 用于降解含木聚糖结构的方法包括通过接触木聚糖酶水解木聚糖的β-1,4-糖苷键，并且进一步解决使用上述水解方法的组合物。

公开(公告)号：US08404477B2

公开(公告)日：2013-03-26

申请号：US12790322

申请日：2010-05-28

申请人： Je-Ruei Liu ,  Ping-Jung Yi

发明人： Je-Ruei Liu ,  Ping-Jung Yi

IPC分类号： C12N1/20

CPC分类号： C12R1/10 ,  A23L5/28 ,  A23L29/065 ,  C12N1/20

摘要： The present invention discloses an isolated pure culture of a novel strain CK1 of Bacillus licheniformis, DSM 025954, capable of detoxification of zearalenone (ZEN). Using physiological, biochemical, morphological identification and 16S rRNA gene sequence analysis methods, the strain CK1 was identified as Bacillus licheniformis. Through extracellular xylanase, CMCase protease assays and evaluations for zearalenone detoxification, the strain CK1 strain was identified to possess good ZEN-detoxifying ability, to be non-hemolytic, non-enterotoxin producing, and displayed high levels of extracellular xylanase, cellulase, and protease activities. Accordingly, Bacillus licheniformis CK1 can be applied as food and feed supplement for bio-detoxification of ZEN.

摘要翻译： 本发明公开了能够解毒玉米赤霉烯酮（ZEN）的地衣芽孢杆菌DSM 025954的新型菌株CK1的分离纯培养物。 利用生理，生化，形态鉴定和16S rRNA基因序列分析方法，鉴定出菌株CK1为地衣芽孢杆菌。 通过细胞外木聚糖酶，CMCase蛋白酶测定和玉米赤霉烯酮解毒评估，鉴定出菌株CK1菌株具有良好的ZEN解毒能力，为非溶血性，非肠毒素产生，并显示高水平的细胞外木聚糖酶，纤维素酶和蛋白酶 活动 因此，地衣芽孢杆菌CK1可作为食品和饲料添加剂用于ZEN的生物解毒。

公开(公告)号：US20110171722A1

公开(公告)日：2011-07-14

申请号：US12790322

申请日：2010-05-28

申请人： Je-Ruei Liu ,  Ping-Jung Yi ,  Cheng-Kang Pai

发明人： Je-Ruei Liu ,  Ping-Jung Yi ,  Cheng-Kang Pai

IPC分类号： C12N1/20 ,  A62D3/02

CPC分类号： C12R1/10 ,  A23L5/28 ,  A23L29/065 ,  C12N1/20

摘要： The present invention discloses an isolated pure culture of a novel strain CK1 of Bacillus licheniformis, BCRC 910458, capable of detoxification of zearalenone (ZEN). Using physiological, biochemical, morphological identification and 16S rRNA gene sequence analysis methods, the strain CK1 was identified as Bacillus licheniformis. Through extracellular xylanase, CMCase protease assays and evaluations for zearalenone detoxification, the strain CK1 strain was identified to possess good ZEN-detoxifying ability, to be non-hemolytic, non-enterotoxin producing, and displayed high levels of extracellular xylanase, cellulase, and protease activities. Accordingly, Bacillus licheniformis CK1 can be applied as food and feed supplement for bio-detoxification of ZEN.

摘要翻译： 本发明公开了能够解毒玉米赤霉烯酮（ZEN）的地衣芽孢杆菌新型菌株CK1（BCRC 910458）的分离纯培养物。 利用生理，生化，形态鉴定和16S rRNA基因序列分析方法，鉴定出菌株CK1为地衣芽孢杆菌。 通过细胞外木聚糖酶，CMCase蛋白酶测定和玉米赤霉烯酮解毒评估，鉴定出菌株CK1菌株具有良好的ZEN解毒能力，为非溶血性，非肠毒素产生，并显示高水平的细胞外木聚糖酶，纤维素酶和蛋白酶 活动 因此，地衣芽孢杆菌CK1可作为食品和饲料添加剂用于ZEN的生物解毒。