&ggr;-phosphoester nucleoside triphosphates
    3.
    发明授权
    &ggr;-phosphoester nucleoside triphosphates 有权
    γ-磷酸酯核苷三磷酸

    公开(公告)号:US06399335B1

    公开(公告)日:2002-06-04

    申请号:US09441108

    申请日:1999-11-16

    IPC分类号: C12Q168

    CPC分类号: C12P19/34

    摘要: The invention provides methods and compositions for polymerizing a particular nucleotide with a polymerase. In general, the method involves (a) forming a mixture of a polymerase and a nucleoside triphosphate (NTP) comprising &agr;, &bgr; and &ggr; phosphates and a &ggr;-phosphate phosphoester-linked functional group; and incubating the mixture under conditions wherein the polymerase catalyzes cleavage of the NTP between the &agr; and &bgr; phosphates, liberating a pyrophosphate comprising the functional group and polymerizing the resultant nucleoside monophosphate, i.e. incorporates the nucleoside monophosphate in a nascent polynucleotide. A variety of functional groups compatible with the polymerization reaction are provided.

    摘要翻译: 本发明提供了用聚合酶聚合特定核苷酸的方法和组合物。 通常,该方法包括(a)形成聚合酶和包含α,β和γ磷酸盐和γ-磷酸磷酸酯连接的官能团的核苷三磷酸(NTP)的混合物; 并且在所述聚合酶催化所述α和β磷酸盐之间的NTP裂解的条件下孵育所述混合物,释放包含所述官能团的焦磷酸盐并聚合所得核苷单磷酸酯,即在新生多核苷酸中掺入核苷单磷酸酯。 提供了与聚合反应相容的各种官能团。

    Control system for vehicles
    5.
    发明申请
    Control system for vehicles 有权
    车辆控制系统

    公开(公告)号:US20070021878A1

    公开(公告)日:2007-01-25

    申请号:US10575223

    申请日:2004-03-25

    IPC分类号: G06F17/00

    摘要: A system for controlling flight of an aircraft has sensors (37, 43), a receiver (45), and a digital control system (57), all of which are carried aboard the aircraft. The sensors (37, 43) determine the position of the aircraft relative to the earth and the inertial movement of the aircraft. The receiver (45) receives transmitted data (51, 55) communicating the position and movement of a reference vehicle relative to the earth. The control system (57) calculates the position and velocity of the aircraft relative to the reference vehicle using the data from the sensors (37, 43) and the receiver (45) and then commands flight control devices (33) on the aircraft for maneuvering the aircraft in a manner that maintains a selected position and/or velocity relative to the reference vehicle. The system allows use of a graphical or tactile user interfaces.

    摘要翻译: 用于控制飞机飞行的系统具有传感器(37,43),接收器(45)和数字控制系统(57),所有这些都被携带在飞行器上。 传感器(37,43)确定飞行器相对于地球的位置和飞行器的惯性运动。 接收器(45)接收传送参考车辆相对于地球的位置和移动的传送数据(51,55)。 控制系统(57)使用来自传感器(37,43)和接收器(45)的数据来计算飞行器相对于参考车辆的位置和速度,然后命令飞行器上的飞行控制装置(33)进行操纵 飞行器以相对于参考车辆保持选定位置和/或速度的方式。 该系统允许使用图形或触觉的用户界面。