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公开(公告)号:US08377715B2
公开(公告)日:2013-02-19
申请号:US12767659
申请日:2010-04-26
申请人: Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
发明人: Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
IPC分类号: G01N1/18
CPC分类号: C12N15/101 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , B01D15/3804
摘要: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.
摘要翻译: 本发明提供了用于从含有细胞碎片的未澄清细胞裂解物中纯化DNA载体的移液管尖端提取柱以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的提取介质床,位于底部玻璃料上方并具有可选的顶部玻璃料。
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公开(公告)号:US20100200509A1
公开(公告)日:2010-08-12
申请号:US12767659
申请日:2010-04-26
申请人: Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
发明人: Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
IPC分类号: B01D15/04
CPC分类号: C12N15/101 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , B01D15/3804
摘要: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.
摘要翻译: 本发明提供了用于从含有细胞碎片的未澄清细胞裂解物中纯化DNA载体的移液管尖端提取柱以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的提取介质床,位于底部玻璃料上方并具有可选的顶部玻璃料。
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公开(公告)号:US08679857B2
公开(公告)日:2014-03-25
申请号:US13752227
申请日:2013-01-28
申请人: Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
发明人: Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
IPC分类号: C12N15/10
CPC分类号: C12N15/101 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , B01D15/3804
摘要: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.
摘要翻译: 本发明提供了用于从含有细胞碎片的未澄清细胞裂解物中纯化DNA载体的移液管尖端提取柱以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的提取介质床,位于底部玻璃料上方并具有可选的顶部玻璃料。
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公开(公告)号:US20130252344A1
公开(公告)日:2013-09-26
申请号:US13752227
申请日:2013-01-28
申请人: Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
发明人: Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
IPC分类号: C12N15/10
CPC分类号: C12N15/101 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , B01D15/3804
摘要: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.
摘要翻译: 本发明提供了用于从含有细胞碎片的未澄清细胞裂解物中纯化DNA载体的移液管尖端提取柱以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的提取介质床,位于底部玻璃料上方并具有可选的顶部玻璃料。
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