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    EXTRACTION SEPARATION USING MAGNETIC BEADS
    1.
    发明申请
    EXTRACTION SEPARATION USING MAGNETIC BEADS 审中-公开
    使用磁珠提取分离

    公开(公告)号：US20150360232A1

    公开(公告)日：2015-12-17

    申请号：US14764919

    申请日：2014-01-29

    Applicant: GE HEALTHCARE BIO-SCIENCES AB

    Inventor： Nils NORRMAN ,  Pierre LE GREVES

    IPC: B03C1/02 ,  C02F1/48

    CPC classification number: B03C1/02 ,  B01D11/0419 ,  B01D11/0423 ,  B01D15/3885 ,  B01J20/24 ,  B01J20/261 ,  B01J20/28009 ,  B01J20/3204 ,  B01J20/3227 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3289 ,  B01J20/3293 ,  B01J2220/64 ,  B03C1/00 ,  B03C1/01 ,  C02F1/48 ,  C02F2101/308 ,  C07K1/14 ,  H01F1/445

    Abstract: A method for purifying a substance in a solution in a simple streamlined process using a magnetic porous particle. For easy small scale purification of a substance, the magnetic porous particle is coated with either a hydrophilic or hydrophobic liquid and transferred into a second liquid containing the substance under conditions which allow said substance to partition into the first liquid within said magnetic porous particle. Finally the magnetic porous particle is removed from said second liquid, wherein the first and second liquid are substantially immiscible and the partition coefficient P of the substance between the first and second liquid is greater than 1.

    Abstract translation: 使用磁性多孔颗粒以简单的流线型方法纯化溶液中的物质的方法。 为了容易地小规模地纯化物质，磁性多孔颗粒用亲水或疏水液体涂覆，并在允许所述物质分配到所述磁性多孔颗粒内的第一液体的条件下转移到含有物质的第二液体中。 最后，从所述第二液体中除去磁性多孔颗粒，其中第一和第二液体基本上是不混溶的，并且第一和第二液体之间物质的分配系数P大于1。

    FIELD SAMPLING KIT FOR CHEMICAL RECOVERY, STORAGE, AND PROFILING, METHOD OF MAKING AND USING THE KIT, AND A DYNAMIC FABRIC PHASE SORPTIVE EXTRACTION (DFPSE) MEDIA
    2.
    发明申请
    FIELD SAMPLING KIT FOR CHEMICAL RECOVERY, STORAGE, AND PROFILING, METHOD OF MAKING AND USING THE KIT, AND A DYNAMIC FABRIC PHASE SORPTIVE EXTRACTION (DFPSE) MEDIA 有权
    用于化学恢复，储存和配置的现场采样工具，制造和使用工具包的方法和动态织物相萃取（DFPSE）介质

    公开(公告)号：US20150258542A1

    公开(公告)日：2015-09-17

    申请号：US14660153

    申请日：2015-03-17

    Applicant: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES

    Inventor： ABUZAR KABIR ,  KENNETH G. FURTON

    IPC: B01L3/00 ,  B01L99/00

    CPC classification number: B01J20/3212 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3276 ,  B01J20/328 ,  B01J20/3285 ,  B01J2220/64 ,  G01N1/405 ,  G01N2001/027

    Abstract: A field kit for collecting analytical samples has one or more dynamic fabric phase sorptive extraction (DFPSE) devices and/or fabric phase sorptive extraction (FPSE) devices and a plurality of containers for storing and transporting the DFPSEs and/or FPSEs that were used for sampling. The field kit has media for documenting information concerning the site, quantity, date, and/or other pertinent information concerning the sampling. Samples can be maintained within the kit for any required period of storage. Sampling can be done once or a plurality of times, such that an initial analysis can be carried out and analysis can be repeated using a portion of a FPSE or with a redundant FPSE that has been stored. The DFPSE device is a sampling device including a plurality of FPSEs, such that a number of different types of analytes can be sampled in different layers of the DFPSE. At least one external surface layer of the DFPSE is a barrier FPSE that restricts solids from underlying layers.

    Abstract translation: 用于收集分析样品的现场试剂盒具有一个或多个动态织物相吸附萃取（DFPSE）装置和/或织物相吸附提取（FPSE）装置和用于储存和运输用于DFPSE和/或FPSE的DFPSE和/或FPSE的多个容器 采样。 现场工具包有媒体记录有关采样的站点，数量，日期和/或其他相关信息的信息。 样品可以保存在试剂盒中以保持任何所需的储存期。 可以进行一次或多次采样，使得可以执行初始分析并且可以使用FPSE的一部分或已经存储的冗余FPSE来重复分析。 DFPSE设备是包括多个FPSE的采样设备，使得可以在DFPSE的不同层中对多个不同类型的分析物进行采样。 DFPSE的至少一个外表面层是限制来自下层的固体的屏障FPSE。

    METHODS, COMPOSITIONS, DEVICES, AND KITS FOR PERFORMING PHOSPHOLIPID SEPARATION
    5.
    发明申请
    METHODS, COMPOSITIONS, DEVICES, AND KITS FOR PERFORMING PHOSPHOLIPID SEPARATION 有权
    用于进行磷脂分离的方法，组合物，装置和工具

    公开(公告)号：US20130053588A1

    公开(公告)日：2013-02-28

    申请号：US13580884

    申请日：2011-05-20

    Applicant: Pamela C. Iraneta ,  Thomas H. Walter ,  Xin Zhang ,  Daniel P. Walsh ,  Kevin D. Wyndham ,  Raymond P. Fish

    Inventor： Pamela C. Iraneta ,  Thomas H. Walter ,  Xin Zhang ,  Daniel P. Walsh ,  Kevin D. Wyndham ,  Raymond P. Fish

    IPC: B01D21/26 ,  B01D21/00 ,  C07F9/02

    CPC classification number: C07F9/103 ,  B01D15/22 ,  B01D15/327 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28019 ,  B01J20/28061 ,  B01J20/28069 ,  B01J20/287 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3246 ,  B01J20/3293 ,  B01J2220/64 ,  B01L3/5023 ,  B01L3/50255 ,  B01L2300/0681 ,  C07K1/30 ,  G01N2030/8813

    Abstract: Methods, kits and devices for separating phospholipids and proteins from small molecules in biochemical samples can feature an apparatus having a wetting barrier, at least one fit and a separation media. For example, an apparatus can include at least one wall defining a chamber having an exit and an entrance; a wetting barrier disposed between the exit and entrance, so as to define a separation media space located between the wetting barrier and the exit and a sample receiving area located between the wetting barrier and the entrance; and a separation media disposed adjacent to the wetting barrier and having a specific affinity for phospholipids. The wetting barrier is adapted to (i) retain the liquid sample and a protein precipitating agent in the sample receiving area under a first force, thereby facilitating the formation of a protein precipitate and a processed sample, and (ii) flow the processed sample through the wetting barrier and separation media under a second force, wherein the second force is greater than the first force, thereby retaining the protein precipitate in the sample receiving area, retaining phospholipids in the separation media, and eluting small molecules.

    Abstract translation: 用于从生物化学样品中分离磷脂和蛋白质与小分子的方法，试剂盒和装置可以具有具有润湿屏障，至少一种配合和分离介质的装置。 例如，设备可以包括限定具有出口和入口的室的至少一个壁; 设置在出口和入口之间的润湿屏障，以便限定位于润湿屏障和出口之间的分离介质空间以及位于润湿屏障和入口之间的样品接收区域; 以及与所述润湿屏障相邻并且对磷脂具有特异性亲和力的分离介质。 润湿屏障适于（i）在第一力下在样品接收区域中保留液体样品和蛋白质沉淀剂，从而有助于蛋白质沉淀物和加工样品的形成，以及（ii）将经处理的样品通过 润湿屏障和分离介质，其中第二力大于第一力，从而将蛋白质沉淀物保留在样品接收区域中，将磷脂保留在分离介质中，并洗脱小分子。

    Method and device for sample preparation
    10.
    发明授权
    Method and device for sample preparation 有权
    样品制备方法和装置

    公开(公告)号：US07722820B2

    公开(公告)日：2010-05-25

    申请号：US11285531

    申请日：2005-11-21

    Applicant: Douglas T. Gjerde ,  Allen Burge ,  Ronald Jones ,  Mark Abel

    Inventor： Douglas T. Gjerde ,  Allen Burge ,  Ronald Jones ,  Mark Abel

    IPC: B01D15/08

    CPC classification number: G01N1/40 ,  B01J20/285 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3244 ,  B01J2220/54 ,  B01J2220/64 ,  B82Y30/00 ,  G01N30/02 ,  G01N30/603 ,  G01N30/6065 ,  G01N2035/1053 ,  Y10T436/25375 ,  Y10T436/255 ,  B01D15/3804

    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.

    Abstract translation: 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床，通常在两个玻璃料之间。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了以疏水和/或亲水膜为玻璃料，和/或通过包含纤维材料的纤维作为芯吸剂的特征的方法。

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