公开(公告)号：US5318895A

公开(公告)日：1994-06-07

申请号：US956716

申请日：1992-10-05

申请人： Jennifer N. Kahn ,  Kevin M. Byrne ,  Louis Kaplan ,  Richard J. Monaghan

发明人： Jennifer N. Kahn ,  Kevin M. Byrne ,  Louis Kaplan ,  Richard J. Monaghan

IPC分类号： C12Q1/02 ,  C12Q1/18 ,  C07D498/00 ,  C12N1/00

CPC分类号： C12R1/685 ,  C12Q1/025 ,  Y10S435/911 ,  Y10S435/913 ,  Y10S435/917

摘要： Disclosed are new Aspergillus niger mutants which can be used for identifying active FK-506/ rapamycin type, or cyclosporin A type inununosuppressants in a broth, natural product extract, or composition exhibiting inununosuppressant activity. Specifically disclosed are the new mutants, ATCC No. 74085 (MF 5659), which is sensitive to FK-506 and rapamycin but resistant to cyclosporin A; ATCC No. 74086 (MF 5659) which is resistant to FK-506, cyclosporin A and rapamycin; and ATCC No. 74087 (MF 5661) which is resistant to FK-506 and rapamycin, but sensitive to cyclosporin A.

摘要翻译： 公开了可以用于鉴定活性FK-506 /雷帕霉素类型的新型黑曲霉突变体，或在肉汤，天然产物提取物或具有抑制剂活性的组合物中的环孢菌素A型抑制剂。 具体公开的是新的突变体ATCC No. 74085（MF5659），其对FK-506和雷帕霉素敏感，但对环孢菌素A具有抗性; 对抗FK-506，环孢菌素A和雷帕霉素的ATCC No.74086（MF5659）; 和对抗FK-506和雷帕霉素但对环孢菌素A敏感的ATCC No.74087（MF5661）。