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    METHOD FOR PREPARING ANTHOCYANIN OLIGOMERS BY USING COENZYME DERIVED FROM ASPERGILLUS SP. STRAIN
    3.
    发明申请
    METHOD FOR PREPARING ANTHOCYANIN OLIGOMERS BY USING COENZYME DERIVED FROM ASPERGILLUS SP. STRAIN 审中-公开

    公开(公告)号：US20180327795A1

    公开(公告)日：2018-11-15

    申请号：US16046012

    申请日：2018-07-26

    申请人： Pyo-Jam Park ,  Tuk-Rai Jeong ,  Hyun-Pil Yang ,  Jin Woo Hwang

    发明人： Pyo-Jam Park ,  Tuk-Rai Jeong ,  Hyun-Pil Yang ,  Jin Woo Hwang

    IPC分类号： C12P17/16 ,  C12N1/16

    CPC分类号： C12P17/162 ,  C12N1/16 ,  C12N9/24 ,  C12P17/06 ,  C12P19/60 ,  C12R1/685

    摘要： The present invention relates to a method of preparing an anthocyanin oligomer using a coenzyme derived from an Aspergillus sp. strain, and more particularly to a method of preparing an anthocyanin oligomer by fermenting an anthocyanin monomer with a coenzyme of Aspergillus niger, which is a kind of Aspergillus sp. strain. According to the present invention, in order to overcome contamination problems during the culturing process using Aspergillus niger, a coenzyme of Aspergillus niger is extracted and the fermentation process is performed using the same, whereby an anthocyanin oligomer characterized by reduced concern of contamination and superior radical-scavenging effects, compared to existing anthocyanin monomers, can be produced. Also, an anthocyanin oligomer, obtained through fermentation using glucosidase as an enzyme contained in the coenzyme, can exhibit excellent fermentation efficiency and radical-scavenging ability, and polymerization of the anthocyanin oligomer can be confirmed even upon the fermentation of the enzyme including glucosidase.

    Use of oxalate deficient aspergillus niger strains for producing a polypeptide
    6.
    发明申请
    Use of oxalate deficient aspergillus niger strains for producing a polypeptide 审中-公开
    使用草酸缺乏黑曲霉菌株产生多肽

    公开(公告)号：US20060127976A1

    公开(公告)日：2006-06-15

    申请号：US10544207

    申请日：2004-02-05

    申请人： Thibaut Wenzel ,  Jean-Marc Ladriere ,  Rogier Meulenberg

    发明人： Thibaut Wenzel ,  Jean-Marc Ladriere ,  Rogier Meulenberg

    IPC分类号： C12P21/06 ,  C12N9/32 ,  C12N1/16 ,  C12N15/74

    CPC分类号： C12R1/685 ,  C12N1/14 ,  C12N15/80 ,  C12P21/02

    摘要： The invention relates to oxalate deficient A. niger strains for the production of a given enzyme, wherein the oxalate deficient strain produces at least the same amount of enzyme as the wild type strain it originates from under the same culture conditions. Preferably, the oxalate deficient A. niger strain produces more enzyme than the wild type strain it originates from under the same culture conditions. More preferably, the oxalate deficient A. niger strain is such that when the strain has been transformed with an expression construct comprising a gene coding for an enzyme, said strain produces at least the amount of the enzyme the wild type strain it originates from would produce under the same culture conditions, when the wild type strain has also been transformed with the same expression construct as the oxalate deficient strain. The invention also relates to method for obtaining such oxalate deficient A. niger strain. The present invention further relates to method for producing an enzyme, wherein an oxalate deficient A. niger strain that produces at least the same amount of enzyme as the wild type strain it originates from under the same culture conditions is used.

    摘要翻译： 本发明涉及用于生产给定酶的草酸盐缺乏的黑曲霉菌株，其中草酸盐缺乏菌株在相同培养条件下产生与野生型菌株至少相同量的酶。 优选地，草酸缺乏的黑曲霉菌株比在相同培养条件下产生的野生型菌株产生更多的酶。 更优选地，草酸缺乏的黑曲霉菌株使得当菌株已经用包含编码酶的基因的表达构建体转化时，所述菌株至少产生其产生的野生型菌株的产生量 在相同的培养条件下，当野生型菌株也用与草酸盐缺乏菌株相同的表达构建体转化时。 本发明还涉及获得这种草酸不足的黑曲霉菌株的方法。 本发明还涉及生产酶的方法，其中使用与相同培养条件下产生的与野生型菌株至少相同量的酶的草酸不足的黑曲霉菌株。