公开(公告)号：US10549010B2

公开(公告)日：2020-02-04

申请号：US12601518

申请日：2008-03-24

申请人： Heeyoung Lee ,  Hyunjin Yang ,  Jun Seok Lee ,  Ji Suk Choi

发明人： Heeyoung Lee ,  Hyunjin Yang ,  Jun Seok Lee ,  Ji Suk Choi

IPC分类号： A61L27/36

摘要： The present invention relates to lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture. More particularly, the present invention relates to a method for preparing lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture, the method comprising the steps of: fragmenting fat tissue to isolate lipids by ultrasonic treatment or high pressure nozzle spray; removing the isolated lipids and fat tissue from which lipids were not isolated to sterilize. According to the present invention, lipids are removed from fat tissue only by physical treatment to maintain the volume thereof and microstructures such as cellular membrane as much as possible and thus the inventive scaffolds are useful for human tissue volume replacement.

公开(公告)号：US20100178681A1

公开(公告)日：2010-07-15

申请号：US12601518

申请日：2008-03-24

申请人： Heeyoung Lee ,  Hyunjin Yang ,  Jun Seok Lee ,  Ji Suk Choi

发明人： Heeyoung Lee ,  Hyunjin Yang ,  Jun Seok Lee ,  Ji Suk Choi

IPC分类号： C12N13/00 ,  C12N5/00

CPC分类号： A61L27/3604 ,  A61L27/3683 ,  A61L2430/40

摘要： The present invention relates to lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture. More particularly, the present invention relates to a method for preparing lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture, the method comprising the steps of: fragmenting fat tissue to isolate lipids by ultrasonic treatment or high pressure nozzle spray; removing the isolated lipids and fat tissue from which lipids were not isolated to sterilize. According to the present invention, lipids are removed from fat tissue only by physical treatment to maintain the volume thereof and microstructures such as cellular membrane as much as possible and thus the inventive scaffolds are useful for human tissue volume replacement.

摘要翻译： 本发明涉及用于人组织体积替换或细胞培养的脂质移除的支架（无脂质支架）。 更具体地说，本发明涉及一种用于制备用于人体组织体积置换或细胞培养的脂质去除的支架（无脂质支架）的方法，所述方法包括以下步骤：通过超声处理或高压喷嘴使脂肪组织分离以分离脂质 喷雾; 去除未分离脂质的分离的脂质和脂肪组织进行灭菌。 根据本发明，仅通过物理处理从脂肪组织中除去脂质以尽可能多地维持其体积和微细结构如细胞膜，因此本发明的支架可用于人体组织体积替换。