Proliferation culture methods using micro-scaffolds for regulations of cell-to-cell signals
    1.
    发明授权
    Proliferation culture methods using micro-scaffolds for regulations of cell-to-cell signals 有权
    使用微支架进行细胞 - 细胞信号调控的增殖培养方法

    公开(公告)号:US08399253B2

    公开(公告)日:2013-03-19

    申请号:US12598032

    申请日:2007-04-28

    申请人: Hyunjin Yang

    发明人: Hyunjin Yang

    IPC分类号: C12N5/02

    CPC分类号: C12N5/0075 C12N2533/40

    摘要: A three-dimensional cell culture method for increasing cell proliferation efficiency by suitably regulating the proliferation-inducing and proliferation-inhibitory signals between cells is provided. The method includes repeatedly performing any one or both of the following processes a) and b) so as to regulate proliferation-inducing and proliferation-inhibitory signals between the cells: a) a process of gradually adding the micro-scaffolds, in which a small amount of the micro-scaffolds are used in an initial stage in order to maintain a suitable distance between the cells, and the amount of the micro-scaffolds is then increased slowly according to cell proliferation rate; and b) a periodic shaking process, in which shaking is performed in order to separate connected cells from each other by a physical force, after the cells are incubated for more than a given period of time.

    摘要翻译: 提供了通过适当调节细胞之间的增殖诱导和增殖抑制信号来提高细胞增殖效率的三维细胞培养方法。 所述方法包括重复进行以下过程a)和b)中的任何一个或两个,以便调节细胞之间的增殖诱导和增殖抑制信号:a)逐渐加入微支架的过程,其中小支架 在初始阶段使用微支架的量以保持细胞之间的适当距离,然后根据细胞增殖速率缓慢增加微支架的量; 和b)周期性振荡过程,其中在细胞孵育超过给定时间段之后,进行摇动以便通过物理力将相互连接的细胞彼此分离。

    SOLAR CELL
    2.
    发明申请
    SOLAR CELL 审中-公开
    太阳能电池

    公开(公告)号:US20120048370A1

    公开(公告)日:2012-03-01

    申请号:US13216061

    申请日:2011-08-23

    IPC分类号: H01L31/0376

    摘要: A solar cell includes a crystalline semiconductor substrate containing a first impurity of a first conductive type, a first non-crystalline impurity semiconductor region directly contacting with the crystalline semiconductor substrate to form a p-n junction with the crystalline semiconductor substrate and including a first portion in which a second impurity of a second conductive type is doped with a first impurity doping concentration and a second portion in which the second impurity is doped with a second impurity doping concentration, the first impurity doping concentration being less than an impurity doping concentration of the crystalline semiconductor substrate and the second impurity doping concentration being greater than the impurity doping concentration of the crystalline semiconductor substrate, a first electrode connected to the first non-crystalline impurity semiconductor region, and a second electrode connected to the crystalline semiconductor substrate.

    摘要翻译: 太阳能电池包括含有第一导电类型的第一杂质的晶体半导体衬底,与晶体半导体衬底直接接触以与晶体半导体衬底形成pn结的第一非结晶杂质半导体区,并且包括第一部分, 第二导电类型的第二杂质掺杂有第一杂质掺杂浓度,第二杂质掺杂有第二杂质掺杂浓度的第二部分,第一杂质掺杂浓度小于晶体半导体的杂质掺杂浓度 衬底,并且第二杂质掺杂浓度大于晶体半导体衬底的杂质掺杂浓度,连接到第一非晶态杂质半导体区的第一电极和连接到晶体半导体衬底的第二电极。

    SOLAR CELL
    3.
    发明申请
    SOLAR CELL 审中-公开
    太阳能电池

    公开(公告)号:US20110056544A1

    公开(公告)日:2011-03-10

    申请号:US12875438

    申请日:2010-09-03

    摘要: A solar cell is disclosed. The solar cell includes a substrate containing first impurities of a first conductive type, an emitter layer containing second impurities of a second conductive type opposite the first conductive type, a first electrode electrically connected to the emitter layer, and a second electrode electrically connected to the substrate. The emitter layer and the substrate form a p-n junction. A doping concentration of the second impurities of the emitter layer linearly or nonlinearly changes depending on a depth of a position within the emitter layer.

    摘要翻译: 公开了一种太阳能电池。 太阳能电池包括含有第一导电类型的第一杂质的基片,含有与第一导电类型相反的第二导电类型的第二杂质的发射极层,与发射极层电连接的第一电极和与该发射极层电连接的第二电极 基质。 发射极层和衬底形成p-n结。 发射极层的第二杂质的掺杂浓度基于发射极层内的位置的深度线性地或非线性地改变。

    Lipid-free scaffolds for human volume replacement or cell culture and use thereof

    公开(公告)号:US10549010B2

    公开(公告)日:2020-02-04

    申请号:US12601518

    申请日:2008-03-24

    IPC分类号: A61L27/36

    摘要: The present invention relates to lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture. More particularly, the present invention relates to a method for preparing lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture, the method comprising the steps of: fragmenting fat tissue to isolate lipids by ultrasonic treatment or high pressure nozzle spray; removing the isolated lipids and fat tissue from which lipids were not isolated to sterilize. According to the present invention, lipids are removed from fat tissue only by physical treatment to maintain the volume thereof and microstructures such as cellular membrane as much as possible and thus the inventive scaffolds are useful for human tissue volume replacement.

    Back contact solar cell and manufacturing method thereof
    6.
    发明授权
    Back contact solar cell and manufacturing method thereof 有权
    背接触太阳能电池及其制造方法

    公开(公告)号:US09082920B2

    公开(公告)日:2015-07-14

    申请号:US13269261

    申请日:2011-10-07

    摘要: A back contact solar cell and a method for manufacturing the back contact solar cell are discussed. The back contact solar cell includes a substrate made of crystalline silicon having a first conductivity type, a passivation layer on one side of the substrate, an antireflection layer on the passivation layer, a first electrode on the other side of the substrate, a second electrode on the other side of the substrate and separated from the first electrode, a first semiconductor layer disposed between the first electrode and the substrate and having the first conductivity type, and a second semiconductor layer disposed between the second electrode and the substrate and having a second conductivity type that is opposite to the first conductivity type. The passivation layer includes at least one of amorphous silicon oxide and amorphous silicon carbide.

    摘要翻译: 讨论了背接触太阳能电池和背接触太阳能电池的制造方法。 背接触太阳能电池包括由基底导电类型的晶体硅构成的衬底,衬底一侧上的钝化层,钝化层上的抗反射层,衬底另一侧的第一电极,第二电极 在所述基板的另一侧并且与所述第一电极分离,设置在所述第一电极和所述基板之间并且具有所述第一导电类型的第一半导体层,以及设置在所述第二电极和所述基板之间的第二半导体层, 导电类型与第一导电类型相反。 钝化层包括非晶硅氧化物和非晶碳化硅中的至少一种。

    BACK CONTACT SOLAR CELL AND MANUFACTURING METHOD THEREOF
    8.
    发明申请
    BACK CONTACT SOLAR CELL AND MANUFACTURING METHOD THEREOF 有权
    返回联系太阳能电池及其制造方法

    公开(公告)号:US20120145233A1

    公开(公告)日:2012-06-14

    申请号:US13269261

    申请日:2011-10-07

    IPC分类号: H01L31/0224 H01L31/0232

    摘要: A back contact solar cell and a method for manufacturing the back contact solar cell are discussed. The back contact solar cell includes a substrate made of crystalline silicon having a first conductivity type, a passivation layer on one side of the substrate, an antireflection layer on the passivation layer, a first electrode on the other side of the substrate, a second electrode on the other side of the substrate and separated from the first electrode, a first semiconductor layer disposed between the first electrode and the substrate and having the first conductivity type, and a second semiconductor layer disposed between the second electrode and the substrate and having a second conductivity type that is opposite to the first conductivity type. The passivation layer includes at least one of amorphous silicon oxide and amorphous silicon carbide.

    摘要翻译: 讨论了背接触太阳能电池和背接触太阳能电池的制造方法。 背接触太阳能电池包括由基底导电类型的晶体硅构成的衬底,衬底一侧上的钝化层,钝化层上的抗反射层,衬底另一侧的第一电极,第二电极 在所述基板的另一侧并且与所述第一电极分离,设置在所述第一电极和所述基板之间并且具有所述第一导电类型的第一半导体层,以及设置在所述第二电极和所述基板之间的第二半导体层, 导电类型与第一导电类型相反。 钝化层包括非晶硅氧化物和非晶碳化硅中的至少一种。

    SCAFFOLDS INCREASED SPECIFIC GRAVITY FOR CELL CULTURE AND METHOD FOR MANUFACTURING THEREOF
    10.
    发明申请
    SCAFFOLDS INCREASED SPECIFIC GRAVITY FOR CELL CULTURE AND METHOD FOR MANUFACTURING THEREOF 审中-公开
    SCAFFOLDS提高细胞培养的特异性重要性及其制造方法

    公开(公告)号:US20100317112A1

    公开(公告)日:2010-12-16

    申请号:US12597895

    申请日:2007-04-27

    IPC分类号: C12N5/02

    摘要: The present invention relates to microtype scaffolds for cell culture, which have their specific gravity increased and a method for manufacturing thereof, and more specifically, relates to microtype scaffolds for cell culture, which have their specific gravity increased, by adding a chemically stable inorganic compound having a high specific gravity in manufacturing biocompatible polymer microtype scaffolds for cell culture and a method for manufacturing thereof. In case where the inventive microtype scaffolds for cell culture is used, it is easy to separate cells cultured on microtype scaffolds, and cell damage can be minimized by reducing separation time, and it is easy to recover cells due to a definite boundary layer.

    摘要翻译: 本发明涉及具有比重增加的细胞培养用微型支架及其制造方法,更具体地,涉及通过添加化学稳定的无机化合物而使其比重增加的细胞培养用微型支架 在生产用于细胞培养的生物相容性聚合物微型支架中具有高比重及其制造方法。 在使用本发明的用于细胞培养的微型支架的情况下,容易分离在微型支架上培养的细胞,并且通过减少分离时间可以最小化细胞损伤,并且由于确定的边界层容易回收细胞。