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公开(公告)号：US09249433B2
公开(公告)日：2016-02-02
申请号：US14386286
申请日：2012-03-20
申请人： Hanjie Ying , Dong Liu , Yong Chen , An Li , Tao Zhou , Jinglan Wu , Xiaoqing Lin , Xiaochun Chen , Jingjing Xie , Jianxin Bai
发明人： Hanjie Ying , Dong Liu , Yong Chen , An Li , Tao Zhou , Jinglan Wu , Xiaoqing Lin , Xiaochun Chen , Jingjing Xie , Jianxin Bai
摘要： The present invention provides Clostridium acetobutylicum and an application thereof. A preservation number of the Clostridium acetobutylicum provided in the invention is CGMCC No. 5234. The Clostridium acetobutylicum provided in the present invention can be used for cogeneration of acetone, butanol, ethanol, and 3-hydroxy butanone through fermentation, so as to improve the economic benefit of butanol fermentation. NAD+ coupling and regeneration can be implemented by adding metabolism or growth regulating substances, so as to improve the product yield, and at the same time, the yield of cogeneration products can be flexibly adjusted, so as to cater for the market demand.
摘要翻译： 本发明提供丙酮丁醇梭菌及其应用。 本发明提供的丙酮丁醇梭菌的保藏号为CGMCC号5234.本发明提供的丙酮丁醇梭菌可通过发酵用于丙酮，丁醇，乙醇和3-羟基丁酮的热电联供，以改善 丁醇发酵的经济效益。 NAD +偶联和再生可以通过添加新陈代谢或生长调节物质来实现，从而提高产品产量，同时可以灵活调整热电产品的产量，以满足市场需求。
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公开(公告)号：US20130158303A1
公开(公告)日：2013-06-20
申请号：US13814490
申请日：2011-04-15
申请人： Hanjie Ying , Xiaoqing Lin , Yong Chen , Xiaochun Chen , Jianxin Bai , Jian Xiong , Wenbin Qian , Jingjing Xie , Jinglan Wu
发明人： Hanjie Ying , Xiaoqing Lin , Yong Chen , Xiaochun Chen , Jianxin Bai , Jian Xiong , Wenbin Qian , Jingjing Xie , Jinglan Wu
IPC分类号： C07C29/76
摘要： Disclosed is a method for separating butanol. The method uses hydrophobic macroporous polymer adsorbent to separate butanol in a mixed solution, and the process comprises the following steps: 1) using macroporous polymer adsorbent to adsorb butanol in a mixed solution; 2) desorbing butanol from macroporous polymer adsorbent. The method is simple; the separation time is short; the efficiency of butanol recovery is high; and the separating cost is low.
摘要翻译： 公开了一种分离丁醇的方法。 该方法使用疏水性大孔聚合物吸附剂在混合溶液中分离丁醇，该方法包括以下步骤：1）使用大孔聚合物吸附剂在混合溶液中吸附丁醇; 2）从大孔聚合物吸附剂中解吸丁醇。 方法简单; 分离时间短; 丁醇回收效率高; 分离成本低。
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3.发明申请METHOD FOR MICROBIAL PRODUCTION OF CYCLIC ADENOSINE 3', 5'-MONOPHOSPHATE 有权用于微生物生产循环腺苷3'，5'-单磷酸酯的方法公开(公告)号：US20130052691A1
公开(公告)日：2013-02-28
申请号：US13634513
申请日：2011-03-10
申请人： Hanjie Ying , Jianxin Bai , He Song , Xiaochun Chen , Jian Xiong , Yong Chen , Jingjing Xie , Jinglan Wu
发明人： Hanjie Ying , Jianxin Bai , He Song , Xiaochun Chen , Jian Xiong , Yong Chen , Jingjing Xie , Jinglan Wu
IPC分类号： C12P19/32
摘要： Disclosed is a method for the production of cyclic adenosine 3′,5′-monophosphate (cAMP) by permeable microbial strain, which uses polyols for protecting activities of enzymes, uses cAMP precursor and phosphate radical ions as substrates, uses glucose as energy provider, and uses metal ions in the mendium. The method is simple, economical and has high yield.
摘要翻译： 公开了一种通过可渗透微生物菌株生产环腺苷3'，5'-单磷酸（cAMP）的方法，其使用多元醇保护酶的活性，使用cAMP前体和磷酸根离子作为底物，使用葡萄糖作为能量提供者， 并在金属中使用金属离子。 该方法简单，经济，成品率高。
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4.发明授权公开(公告)号：US09416385B2
公开(公告)日：2016-08-16
申请号：US13634513
申请日：2011-03-10
申请人： Hanjie Ying , Jianxin Bai , He Song , Xiaochun Chen , Jian Xiong , Yong Chen , Jingjing Xie , Jinglan Wu
发明人： Hanjie Ying , Jianxin Bai , He Song , Xiaochun Chen , Jian Xiong , Yong Chen , Jingjing Xie , Jinglan Wu
摘要： The invention relates to a method for the production of adenosine 3′,5′-monophosphate (cAMP) by a permeable microbial strain which uses polyols for protecting activities of enzymes and a cAMP precursor and phosphate as substrates. Glucose is used as an energy provider and metal ions and an organic solvent such as acetone are used in the medium.
摘要翻译： 本发明涉及通过可渗透的微生物菌株生产腺苷3'，5'-单磷酸（cAMP）的方法，该方法使用多元醇来保护酶活性，cAMP前体和磷酸盐作为底物。 葡萄糖用作能量提供者，介质中使用金属离子和有机溶剂如丙酮。
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公开(公告)号：US08766019B2
公开(公告)日：2014-07-01
申请号：US13814490
申请日：2011-04-15
申请人： Hanjie Ying , Xiaoqing Lin , Yong Chen , Xiaochun Chen , Jianxin Bai , Jian Xiong , Wenbin Qian , Jingjing Xie , Jinglan Wu
发明人： Hanjie Ying , Xiaoqing Lin , Yong Chen , Xiaochun Chen , Jianxin Bai , Jian Xiong , Wenbin Qian , Jingjing Xie , Jinglan Wu
IPC分类号： C07C29/74
摘要： Disclosed is a method for separating butanol. The method uses hydrophobic macroporous polymer adsorbent to separate butanol in a mixed solution, and the process comprises the following steps: 1) using macroporous polymer adsorbent to adsorb butanol in a mixed solution; 2) desorbing butanol from macroporous polymer adsorbent. The method is simple; the separation time is short; the efficiency of butanol recovery is high; and the separating cost is low.
摘要翻译： 公开了一种分离丁醇的方法。 该方法使用疏水性大孔聚合物吸附剂在混合溶液中分离丁醇，该方法包括以下步骤：1）使用大孔聚合物吸附剂在混合溶液中吸附丁醇; 2）从大孔聚合物吸附剂中解吸丁醇。 方法简单; 分离时间短; 丁醇回收效率高; 分离成本低。
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6.发明申请METHOD FOR DESORBING AND REGENERATING BUTANOL-ADSORBING HYDROPHOBIC MACROPOROUS POLYMER ADSORBENT 有权用于吸附和再生苯酚 - 吸附的疏水性大分子聚合物吸附剂的方法公开(公告)号：US20140155658A1
公开(公告)日：2014-06-05
申请号：US14130887
申请日：2011-07-05
申请人： Hanjie Ying , Xiaoqing Lin , Jianshen Fan , Jinglan Wu , Yong Chen , Xiaochun Chen , Jinglin Xie , Jian Xiong , Jianxin Bai
发明人： Hanjie Ying , Xiaoqing Lin , Jianshen Fan , Jinglan Wu , Yong Chen , Xiaochun Chen , Jinglin Xie , Jian Xiong , Jianxin Bai
IPC分类号： C07C29/76
CPC分类号： C07C29/76 , B01D15/203 , B01D15/426 , B01J20/3425 , B01J20/3475 , C08J7/02 , C08J11/02 , C08J2325/04 , C07C31/12
摘要： The present invention provides a method for desorbing and regenerating a butanol-adsorbing hydrophobic macroporous polymer adsorbent, comprising: successively eluting the hydrophobic macroporous polymer adsorbent with butanol adsorbed therein using a water soluble low-boiling-point polar solvent and water. After a butanol-containing solution, such as a butanol fermentation liquor, has been adsorbed by a hydrophobic macroporous polymer adsorbent, the butanol adsorbed in the adsorbent can be thoroughly dissolved and removed by the desorption and regeneration method of the present invention; furthermore, the adsorbent can be directly regenerated to recover its adsorption capability on a fixed bed adsorbing the butanol fermentation liquor, wherein the next stage of adsorption process can be directly entered without taking the adsorbent out from the fixed bed; therefore it saves a great deal of time and improves the regeneration efficiency. The method provided in the present invention has a simple process, a short separation time, easy, fast and complete desorption and regeneration, low equipment investmentand pollution, and reduced energy consumption, and therefore production is easy on a large scale.
摘要翻译： 本发明提供了一种用于解吸和再生丁醇吸附的疏水性大孔聚合物吸附剂的方法，其包括：使用水溶性低沸点极性溶剂和水连续洗脱其中吸附有丁醇的疏水性大孔聚合物吸附剂。 在丁醇发酵液等丁醇溶液被疏水性大孔聚合物吸附剂吸附后，通过本发明的解吸和再生方法可以使吸附剂中吸附的丁醇充分溶解除去， 此外，可以直接再生吸附剂以在吸附丁醇发酵液的固定床上回收其吸附能力，其中可以直接进入下一阶段的吸附过程而不将吸附剂从固定床中取出; 因此节省了大量的时间，提高了再生效率。 本发明提供的方法工艺简单，分离时间短，解吸和再生容易，快速，完整，设备投资少，污染少，能耗低，生产规模大。
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公开(公告)号：US20150093796A1
公开(公告)日：2015-04-02
申请号：US14386286
申请日：2012-03-20
申请人： Hanjie Ying , Dong Liu , Yong Chen , Yong Chen , An Li , Tao Zhou , Jinglan Wu , Xiaoqing Lin , Xiaochun Chen , Jingjing Xie , Jianxin Bai
发明人： Hanjie Ying , Dong Liu , Yong Chen , Yong Chen , An Li , Tao Zhou , Jinglan Wu , Xiaoqing Lin , Xiaochun Chen , Jingjing Xie , Jianxin Bai
摘要： The present invention provides Clostridium acetobutylicum and an application thereof. A preservation number of the Clostridium acetobutylicum provided in the invention is CGMCC No. 5234. The Clostridium acetobutylicum provided in the present invention can be used for cogeneration of acetone, butanol, ethanol, and 3-hydroxy butanone through fermentation, so as to improve the economic benefit of butanol fermentation. NAD+ coupling and regeneration can be implemented by adding metabolism or growth regulating substances, so as to improve the product yield, and at the same time, the yield of cogeneration products can be flexibly adjusted, so as to cater for the market demand.
摘要翻译： 本发明提供丙酮丁醇梭菌及其应用。 本发明提供的丙酮丁醇梭菌的保藏号为CGMCC号5234.本发明提供的丙酮丁醇梭菌可通过发酵用于丙酮，丁醇，乙醇和3-羟基丁酮的热电联供，以改善 丁醇发酵的经济效益。 NAD +偶联和再生可以通过添加新陈代谢或生长调节物质来实现，从而提高产品产量，同时可以灵活调整热电产品的产量，以满足市场需求。
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8.发明授权Method for desorbing and regenerating butanol-adsorbing hydrophobic macroporous polymer adsorbent 有权解吸和再生丁醇吸附疏水性大孔聚合物吸附剂的方法公开(公告)号：US09108897B2
公开(公告)日：2015-08-18
申请号：US14130887
申请日：2011-07-05
申请人： Hanjie Ying , Xiaoqing Lin , Jiansheng Fan , Jinglan Wu , Yong Chen , Xiaochun Chen , Jingjing Xie , Jian Xiong , Jianxin Bai
发明人： Hanjie Ying , Xiaoqing Lin , Jiansheng Fan , Jinglan Wu , Yong Chen , Xiaochun Chen , Jingjing Xie , Jian Xiong , Jianxin Bai
CPC分类号： C07C29/76 , B01D15/203 , B01D15/426 , B01J20/3425 , B01J20/3475 , C08J7/02 , C08J11/02 , C08J2325/04 , C07C31/12
摘要： The present invention provides a method for desorbing and regenerating a butanol-adsorbing hydrophobic macroporous polymer adsorbent, comprising: successively eluting the hydrophobic macroporous polymer adsorbent with butanol adsorbed therein using a water soluble low-boiling-point polar solvent and water. The method provided in the present invention has a simple process, a short separation time, easy, fast and complete desorption and regeneration, low equipment investment and pollution, and reduced energy consumption, and therefore production is easy on a large scale.
摘要翻译： 本发明提供了一种用于解吸和再生丁醇吸附的疏水性大孔聚合物吸附剂的方法，其包括：使用水溶性低沸点极性溶剂和水连续洗脱其中吸附有丁醇的疏水性大孔聚合物吸附剂。 本发明提供的方法工艺简单，分离时间短，解吸和再生容易，快速，完整，设备投资少，污染少，能耗低等特点，生产容易。
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公开(公告)号：US20130090466A1
公开(公告)日：2013-04-11
申请号：US13702039
申请日：2011-04-21
申请人： Hanjie Ying , Wenbin Qian , Yong Chen , Xiaochun Chen , Jianxin Bai , Jian Xiong , Xiaoqing Lin , Jingjing Xie , Jinglan Wu
发明人： Hanjie Ying , Wenbin Qian , Yong Chen , Xiaochun Chen , Jianxin Bai , Jian Xiong , Xiaoqing Lin , Jingjing Xie , Jinglan Wu
IPC分类号： C07H19/16
CPC分类号： C07H19/16 , C07H1/06 , C07H19/213
摘要： Provided is a crystallization process of cyclic adenosine 3′,5′-monophosphate, which comprises the following steps: 1) reacting an aqueous solution of cyclic adenosine 3′,5′-monophosphate with a base to obtain a salt of cyclic adenosine 3′,5′-monophosphate; 2) reacting the cyclic adenosine 3′,5′-monophosphate salt solution obtained in step 1) with an acid to obtain cyclic adenosine 3′,5′-monophosphate; 3) keeping cyclic adenosine 3′,5′-monophosphate obtained in step 2) at 0-15° C.
摘要翻译： 提供环腺苷3'，5'-单磷酸的结晶过程，其包括以下步骤：1）使环腺苷3'，5'-单磷酸的水溶液与碱反应，得到环腺苷3' ，5'-单磷酸 2）使步骤1）中得到的环状腺苷3'，5'-单磷酸盐溶液与酸反应，得到环状腺苷3'，5'-单磷酸; 3）在0-15℃保持步骤2）中获得的环腺苷3'，5'-单磷酸
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