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    Antifungal proteins and methods for their use
    4.
    发明授权
    Antifungal proteins and methods for their use 有权
    抗真菌蛋白及其使用方法

    公开(公告)号：US06573361B1

    公开(公告)日：2003-06-03

    申请号：US09732210

    申请日：2000-12-07

    申请人： Greg J. Bunkers ,  Jihong Liang ,  Cindy A. Mittanck ,  Jeffrey W. Seale ,  Yonnie S. Wu

    发明人： Greg J. Bunkers ,  Jihong Liang ,  Cindy A. Mittanck ,  Jeffrey W. Seale ,  Yonnie S. Wu

    IPC分类号： A61K3800

    CPC分类号： C12N15/8282 ,  A01N63/04 ,  A61K38/00

    摘要： A novel protein was isolated from Fusarium culmorum and characterized. The protein, termed FCWP1, demonstrated significant antifungal activity against several fungal species. Mutations in proteolytic consensus sequences contained within FCWP1 improved the stability of its antifungal activity. In addition, a class of proteins related to FCWP1 was identified and characterized. This class is made up of ribosomal proteins and displayed similar values for pI and molecular weight. A representative number of proteins from this class were tested and found to have significant antifungal activities. The antifungal proteins disclosed herein are useful in controlling fungal infections in plants. Transgenic plants may be produced that are more resistant to fungal infections relative to non-transgenic plants of the same species. Alternatively, the proteins may be applied to plants exogenously.

    摘要翻译： 从镰刀菌中分离出一种新的蛋白质并进行了表征。 称为FCWP1的蛋白质表现出对几种真菌物种的显着抗真菌活性。 FCWP1中包含的蛋白水解共有序列的突变改善了其抗真菌活性的稳定性。 此外，鉴定和表征了一类与FCWP1相关的蛋白质。 该类由核糖体蛋白质组成，显示出与pI和分子量相似的值。 测试了来自该类别的代表性的蛋白质，并发现其具有显着的抗真菌活性。本文公开的抗真菌蛋白质可用于防治植物中的真菌感染。 可以产生相对于相同物种的非转基因植物更能抵抗真菌感染的转基因植物。 或者，蛋白质可以外源地施用于植物。

    High Protein Soybean Meal
    6.
    发明申请
    High Protein Soybean Meal 审中-公开
    高蛋白大豆粉

    公开(公告)号：US20090041887A1

    公开(公告)日：2009-02-12

    申请号：US10587114

    申请日：2005-01-26

    申请人： Jihong Liang ,  Fang Chi ,  Douglas C. Kotowski

    发明人： Jihong Liang ,  Fang Chi ,  Douglas C. Kotowski

    IPC分类号： A23K1/14 ,  C07K14/415

    CPC分类号： C12N15/8275 ,  A23K10/30 ,  A23K50/10 ,  A23K50/30 ,  A23K50/75

    摘要： A high protein soybean meal is disclosed. The soybean meal is generated from soybeans that are capable of commercial yields, wherein the meal comprises at least 58% protein on a dry weight basis. The soybean meal of the present invention may also be generated from soybeans comprising a mean whole seed total protein plus oil content of greater than about 64%, on a dry weight basis, wherein the soybean has a yield, under standard agronomic conditions, of at least 30 bushels per acre. Also disclosed is an animal feed containing the soybean meal of the present invention.

    摘要翻译： 公开了高蛋白豆粕。 大豆粉由能够获得商业产量的大豆产生，其中该膳食以干重为基础包含至少58％的蛋白质。 本发明的大豆粉也可以由大豆产生，所述大豆以干重计基本上包含平均全种子总蛋白加上大于约64％的油含量，其中大豆在标准农学条件下的产量为 每英亩至少30蒲式耳。 还公开了含有本发明的豆粕的动物饲料。

    HIGH TRYPTOPHAN SOYBEAN MEAL
    7.
    发明申请
    HIGH TRYPTOPHAN SOYBEAN MEAL 审中-公开
    高三木豆大豆食品

    公开(公告)号：US20090011084A1

    公开(公告)日：2009-01-08

    申请号：US11995066

    申请日：2006-07-06

    申请人： Jihong Liang ,  Fang Chi

    发明人： Jihong Liang ,  Fang Chi

    IPC分类号： A23K1/14 ,  A23L1/20

    CPC分类号： C12N15/8254 ,  A23K20/142 ,  A23K50/30 ,  A23K50/75 ,  A23K50/80 ,  Y02A40/818

    摘要： The present invention is directed to a soybean meal with high tryptophan content and its method of manufacture. The high tryptophan content soybean meal is to be used as an ingredient in animal feeding operations. Also provided are products from the further processing of the soybean meal.

    摘要翻译： 本发明涉及具有高色氨酸含量的豆粕及其制造方法。 高色氨酸含量的大豆粉作为动物饲养操作中的一种成分。 还提供了进一步加工大豆粉的产品。

    Arcelin-5 promoter and uses thereof
    8.
    发明授权
    Arcelin-5 promoter and uses thereof 有权
    Arcelin-5启动子及其用途

    公开(公告)号：US06927321B2

    公开(公告)日：2005-08-09

    申请号：US10015637

    申请日：2001-12-17

    申请人： Qi Wang ,  Patrice Dubois ,  Jihong Liang ,  Tim Oulmassov

    发明人： Qi Wang ,  Patrice Dubois ,  Jihong Liang ,  Tim Oulmassov

    IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  C12N5/82 ,  A01H5/00 ,  C12N5/04 ,  C12N15/11

    CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8234

    摘要： The present invention discloses Arcelin promoters capable of transcribing a heterologous nucleic acid sequence at high levels in plants. The promoters are particularly suited for use in soybean plants and plant cells. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the high-expression Arcelin promoters, and methods for preparing and using the same.

    摘要翻译： 本发明公开了能够在植物中以高水平转录异源核酸序列的佐剂启动子。 启动子特别适用于大豆植物和植物细胞。 还公开了改性，制备和使用促进剂的方法。 本发明进一步公开了组合物，转化的宿主细胞，转基因植物和含有高表达Arcelin启动子的种子及其制备和使用方法。

    Nucleic acid sequences encoding an antifungal polypeptide, aly AFP from alyssum and methods for their use
    10.
    发明授权
    Nucleic acid sequences encoding an antifungal polypeptide, aly AFP from alyssum and methods for their use 失效
    编码抗真菌多肽的核酸序列，来自alyssum的aly AFP及其使用方法

    公开(公告)号：US06215048B1

    公开(公告)日：2001-04-10

    申请号：US09103489

    申请日：1998-06-24

    申请人： Jihong Liang ,  Dilip Maganlal Shah ,  Yonnie Shun Wu ,  Cindy Annette Rosenberger

    发明人： Jihong Liang ,  Dilip Maganlal Shah ,  Yonnie Shun Wu ,  Cindy Annette Rosenberger

    IPC分类号： A01H500

    CPC分类号： C07K14/415 ,  A01N65/00 ,  A01N65/38 ,  C12N15/8282

    摘要： Isolated DNA molecules encoding an AlyAFP antifungal polypeptide from Alyssum are disclosed. The isolated DNA molecules comprise the nucleotide sequence of SEQ ID NO:12 or encode the amino acid sequence of SEQ ID NO:2. Also disclosed are vectors and transgenic plants comprising said DNA molecules and a method for reducing fungal damage in a plant with said DNA molecule. The isolated DNA molecules may be used to prepare transgenic plants that exhibit reduced damage from fungi as compared to non-transgenic plants. The isolated DNA molecules may be used in combination with additional nucleic acid sequences encoding anti-fungal or anti-insect proteins or polypeptides.

    摘要翻译： 公开了从Alyssum编码AlyAFP抗真菌多肽的分离的DNA分子。 分离的DNA分子包含SEQ ID NO：12的核苷酸序列或编码SEQ ID NO：2的氨基酸序列。 还公开了包含所述DNA分子的载体和转基因植物以及用所述DNA分子减少植物中真菌损伤的方法。 分离的DNA分子可用于制备与非转基因植物相比表现出来自真菌的减少的损伤的转基因植物。 分离的DNA分子可以与编码抗真菌或抗昆虫蛋白质或多肽的其它核酸序列组合使用。