Internal ribosome entry sites for recombinant protein expression
    2.
    发明申请
    Internal ribosome entry sites for recombinant protein expression 审中-公开
    用于重组蛋白表达的内部核糖体进入位点

    公开(公告)号:US20050112095A1

    公开(公告)日:2005-05-26

    申请号:US10614283

    申请日:2003-07-08

    摘要: The invention describes compositions and methods for recombinant protein expression in a wide range of cell types, including mammalian, insect, and bacterial cells. The compositions comprise a viral IRES sequence selected from enterovirus 71 (EV71), hepatitis C virus (HCV), or encephalomyocarditis virus (EMCV), or a variant or fragment thereof, or alternatively, a homolog of a viral IRES selected from EV71, HCV, or EMCV, or a variant or fragment thereof. Methods of using the compositions are also described.

    摘要翻译: 本发明描述了在广泛范围的细胞类型(包括哺乳动物,昆虫和细菌细胞)中重组蛋白表达的组合物和方法。 组合物包含选自肠病毒71(EV71),丙型肝炎病毒(HCV)或脑心肌炎病毒(EMCV)或其变体或片段的病毒IRES序列,或者选自EV71,HCV的病毒IRES的同源物 ,或EMCV,或其变体或片段。 还描述了使用组合物的方法。