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17 条记录 (耗时 0.023 秒)

    Internal ribosome entry sites for recombinant protein expression
    1.
    发明申请
    Internal ribosome entry sites for recombinant protein expression 审中-公开
    用于重组蛋白表达的内部核糖体进入位点

    公开(公告)号：US20050112095A1

    公开(公告)日：2005-05-26

    申请号：US10614283

    申请日：2003-07-08

    申请人： Tsu-An Hsu ,  Tzong-Yuan Wu ,  Jin-Ching Lee

    发明人： Tsu-An Hsu ,  Tzong-Yuan Wu ,  Jin-Ching Lee

    IPC分类号： A61K48/00 ,  C07K14/085 ,  C12N15/85 ,  C12N15/86 ,  C12N15/861 ,  C12N15/866 ,  C12N15/867

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N15/85 ,  C12N2710/14145 ,  C12N2770/32322 ,  C12N2840/203

    摘要： The invention describes compositions and methods for recombinant protein expression in a wide range of cell types, including mammalian, insect, and bacterial cells. The compositions comprise a viral IRES sequence selected from enterovirus 71 (EV71), hepatitis C virus (HCV), or encephalomyocarditis virus (EMCV), or a variant or fragment thereof, or alternatively, a homolog of a viral IRES selected from EV71, HCV, or EMCV, or a variant or fragment thereof. Methods of using the compositions are also described.

    摘要翻译： 本发明描述了在广泛范围的细胞类型（包括哺乳动物，昆虫和细菌细胞）中重组蛋白表达的组合物和方法。 组合物包含选自肠病毒71（EV71），丙型肝炎病毒（HCV）或脑心肌炎病毒（EMCV）或其变体或片段的病毒IRES序列，或者选自EV71，HCV的病毒IRES的同源物 ，或EMCV，或其变体或片段。 还描述了使用组合物的方法。

    Chimeral internal ribosomal entry site sequence and uses thereof
    7.
    发明授权
    Chimeral internal ribosomal entry site sequence and uses thereof 有权
    雌性内部核糖体进入序列及其用途

    公开(公告)号：US08367404B2

    公开(公告)日：2013-02-05

    申请号：US13046744

    申请日：2011-03-13

    申请人： Tzong-Yuan Wu ,  Ming-Kun Liu

    发明人： Tzong-Yuan Wu ,  Ming-Kun Liu

    IPC分类号： C12N15/00 ,  C07H21/04 ,  C12N5/00

    CPC分类号： C12N15/86 ,  C12N2710/14143 ,  C12N2840/203

    摘要： An improved baculovirus vector capable of expressing genes in mammalian or insect host cells, and the uses thereof are disclosed. The improved baculovirus vector includes in sequence: a promoter; a first nucleic acid operably linked to the promoter for expressing a first protein in the mammalian or insect host cells; a chimera internal ribosomal entry site (IRES) comprising a portion of an enterovirus (EV) IRES sequence at least 90% identical to SEQ ID NO: 1 and a portion of a Rhopalosiphum padi virus (RhPV) IRES sequence at least 90% identical to SEQ ID NO: 2; and a second nucleic acid operable linked to the portion of the RhPV IRES sequence for expressing a second protein in the mammalian or insect host cells.

    摘要翻译： 公开了能够在哺乳动物或昆虫宿主细胞中表达基因的改良的杆状病毒载体及其用途。 改良的杆状病毒载体依次包括：启动子; 与所述启动子可操作地连接以在哺乳动物或昆虫宿主细胞中表达第一蛋白质的第一核酸; 嵌合体内部核糖体进入位点（IRES），其包含与SEQ ID NO：1至少90％相同的一部分肠病毒（EV）IRES序列和一部分与Rhopalosiphum padi病毒（RhPV）IRES序列至少90％相同的 SEQ ID NO：2; 以及可操作地连接到所述RhPV IRES序列的部分以在哺乳动物或昆虫宿主细胞中表达第二蛋白质的第二核酸。

    METHOD FOR FABRICATING AEROGELS AND THEIR APPLICATIONS IN BIOCOMPOSITES
    8.
    发明申请
    METHOD FOR FABRICATING AEROGELS AND THEIR APPLICATIONS IN BIOCOMPOSITES 有权
    用于制备空白样品的方法及其在生物聚合物中的应用

    公开(公告)号：US20110065820A1

    公开(公告)日：2011-03-17

    申请号：US12948124

    申请日：2010-11-17

    申请人： Yui-Whei Chen-Yang ,  Yen-Kuang Li ,  Ching-Yao Yuan ,  Tzong-Yuan Wu

    发明人： Yui-Whei Chen-Yang ,  Yen-Kuang Li ,  Ching-Yao Yuan ,  Tzong-Yuan Wu

    IPC分类号： C08J9/00 ,  B82Y30/00

    CPC分类号： C12N11/14 ,  C01B33/1585 ,  C07D233/56 ,  C07D233/58

    摘要： The present invention discloses a method for fabricating aerogels, a method for fabricating surface-modified aerogels, and a method for fabricating biocomposites. Take the fabricating method of biocomposites for example, first, a precursor solution is provided and the precursor solution comprises a hydrophilic ionic liquid, a catalyzed hydrolysis and/or condensation reagent, at least one biomolecule. Next, a curing process is performed for the precursor solution to hydrolyze and polymerize the at least one alkoxide monomer and/or aryloxide monomer to wrap at least one biomolecule and thus form biocomposite. Afterwards, an extracting process is performed by a solvent for the biocomposite to substitute the ionic liquid in the biocomposite. Finally, a drying process for the biocomposite is carried out after the extracting process so as to remove the solvent in the biocomposite. Therefore, the biocomposite is formed.

    摘要翻译： 本发明公开了一种制造气凝胶的方法，一种制造表面改性气凝胶的方法和一种制造生物复合材料的方法。 以生物复合材料的制造方法为例，首先提供前体溶液，前体溶液包含亲水离子液体，催化水解和/或缩合试剂，至少一种生物分子。 接下来，对前体溶液进行固化过程以水解和聚合至少一种醇盐单体和/或芳醚单体以包裹至少一种生物分子，从而形成生物复合物。 之后，通过用于生物复合物的溶剂进行萃取处理以代替生物复合材料中的离子液体。 最后，在提取过程之后进行生物复合物的干燥过程，以除去生物复合材料中的溶剂。 因此，形成生物复合材料。

    Isolated Polynucleotide Sequence with IRES Activity
    9.
    发明申请
    Isolated Polynucleotide Sequence with IRES Activity 审中-公开
    具有IRES活性的分离的多核苷酸序列

    公开(公告)号：US20100223683A1

    公开(公告)日：2010-09-02

    申请号：US12778408

    申请日：2010-05-12

    申请人： Tzong-Yuan Wu ,  Chung-Hsiung Wang ,  Chih-Yu Wu ,  Ying-Ju Chen

    发明人： Tzong-Yuan Wu ,  Chung-Hsiung Wang ,  Chih-Yu Wu ,  Ying-Ju Chen

    IPC分类号： A01K67/033 ,  C12N15/74 ,  C12N5/10

    CPC分类号： C12N15/86 ,  C12N2770/32043 ,  C12N2830/00 ,  C12N2830/60 ,  C12N2840/203

    摘要： Provided herein is an isolated polynucleotide sequence with internal ribosome entry site (IRES) activity, which directs translation initiation in an insect expression system in a cap-independent manner. In particular, the invention relates to an isolated polynucleotide comprises the 5′ UTR of perina nuda Picorna-like virus (PnV) that possesses IRES activity. Methods of identifying a polynucleotide with IRES activity and methods of expressing at least two polypeptides in an insect system are also disclosed herein.

    摘要翻译： 本文提供了具有内部核糖体进入位点（IRES）活性的分离的多核苷酸序列，其以帽独立的方式指导昆虫表达系统中的翻译起始。 特别地，本发明涉及包含具有IRES活性的perina nuda Picorna样病毒（PnV）的5'UTR的分离的多核苷酸。 鉴定具有IRES活性的多核苷酸的方法和在昆虫系统中表达至少两种多肽的方法也在本文中公开。