公开(公告)号：US5447858A

公开(公告)日：1995-09-05

申请号：US599993

申请日：1984-04-13

申请人： Joe L. Key ,  William B. Gurley ,  Ronald T. Nagao ,  Friedrich Schoeffl ,  Eva Czarnecka

发明人： Joe L. Key ,  William B. Gurley ,  Ronald T. Nagao ,  Friedrich Schoeffl ,  Eva Czarnecka

IPC分类号： A01H1/00 ,  C07K14/325 ,  C12N1/20 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12N5/14 ,  C12N15/29 ,  C12N15/32

CPC分类号： C12N15/8237 ,  C07K14/325

摘要： A plant heat shock promoter has been isolated from soybean, sequenced and shown to induce gene expression in response to a temporary heat shock. This invention utilizes the disclosed plant heat shock promoters for the construction of promoter/structural gene chimeras which are expressible in plant cells in response to heat shock. The reporter gene .beta.-galactosidase was expressed transiently in response to a heat shock signal. Further, this invention permits application of heat shock inducible chimeras as a detection method for successful transformation of plant cells.

摘要翻译： 已经从大豆中分离出植物热休克启动子，测序并显示出诱导基因表达以响应临时热休克。 本发明利用所公开的植物热休克启动子来构建启动子/结构基因嵌合体，其可以在植物细胞中响应于热休克而表达。 报道基因β-半乳糖苷酶是响应于热休克信号瞬时表达的。 此外，本发明允许应用热休克诱导型嵌合体作为植物细胞成功转化的检测方法。