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公开(公告)号：US06329191B1
公开(公告)日：2001-12-11
申请号：US08113890
申请日：1993-08-30
申请人： John M. Ivy , David E. Clements
发明人： John M. Ivy , David E. Clements
IPC分类号： C12N1556
CPC分类号： C12N9/2465 , C07K16/40
摘要： A recombinant DNA encoding coffee bean &agr;-galactosidase permits the production of purified forms of this protein. The protein is useful in converting human Type B red blood cells into cells physiologically similar to Type O red blood cells. The availability of this enzyme permits more effective conversion than use of &agr;-galactosidase from other sources.
摘要翻译： 编码咖啡豆α-半乳糖苷酶的重组DNA允许产生纯化形式的该蛋白质。 该蛋白质可用于将人类B型红细胞转化为与O型红细胞生理相似的细胞。 与使用其他来源的α-半乳糖苷酶相比，该酶的可用性允许更有效的转化。
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公开(公告)号：US5695965A
公开(公告)日：1997-12-09
申请号：US896455
申请日：1992-06-02
申请人： W. Dorsey Stuart , John M. Ivy , Kenneth Koo
发明人： W. Dorsey Stuart , John M. Ivy , Kenneth Koo
CPC分类号： C12N9/6481 , C12N15/80
摘要： Neurospora crassa transformations are employed for production of heterologous proteins, where DNA constructs are prepared using a foreign gene under transcriptional and translational regulatory regions functional in Neurospora. A functional signal sequence is provided for secretion of the heterologous gene product. An auxotrophic Neurospora host is employed and transformed by the combination of the subject construct and a complementing gene. The resulting transformants provide for efficient secretion of the heterologous product.
摘要翻译： 粗糙脉孢菌转化用于生产异源蛋白质，其中使用外源基因在神经丝孢菌中具有功能的转录和翻译调节区制备DNA构建体。 提供功能信号序列用于分泌异源基因产物。 使用营养缺陷型神经孢子体宿主并通过受试者构建体和补体基因的组合进行转化。 得到的转化体提供异源产物的有效分泌。
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公开(公告)号：US06749857B1
公开(公告)日：2004-06-15
申请号：US09376463
申请日：1999-08-18
IPC分类号： A01J2100
CPC分类号： C07K14/005 , A61K39/00 , C07K2319/00 , C12N2770/24122 , Y02A50/386
摘要： The present invention discloses and claims vaccines containing, as an active ingredient, a secreted recombinantly produced dimeric form of truncated flaviviral envelope protein. The vaccines are capable of eliciting the production of neutralizing antibodies against flaviviruses. The dimeric forms of truncated flaviviral envelope protein are formed 1) by directly linking two tandem copies of 80% E in a head to tail fashion via a flexible tether; 2) via the formation of a leucine zipper domain through the homodimeric association of two leucine zipper helices each fused to the carboxy terminus of an 80% E molecule; or 3) via the formation of a non-covalently associated four-helix bundle domain formed upon association of two helix-turn-helix moieties each attached to the carboxy terminus of an 80% E molecule. All products are expressed as a polyprotein including prM and the modified 80% E products are secreted from Drosophila melanogaster Schneider 2 cells using the human tissue plasminogen activator secretion signal sequence (tPAL). Secreted products are generally more easily purified than those expressed intracellularly, facilitating vaccine production. One embodiment of the present invention is directed to a vaccine for protection of a subject against infection by dengue virus. The vaccine contains, as active ingredient, the dimeric form of truncated envelope protein of a dengue virus serotype. The dimeric truncated E is secreted as a recombinantly produced protein from eucaryotic cells. The vaccine may further contain portions of additional dengue virus serotype dimeric E proteins similarly produced. Another embodiment of the present invention is directed to methods to utilize the dimeric form of truncated dengue envelope protein for diagnosis of infection in individuals at risk for the disease. The diagnostic contains, as active ingredient, the dimeric form of truncated envelope protein of a dengue virus serotype. The dimeric truncated E is secreted as a recombinantly produced protein from eucaryotic cells. The diagnostic may further contain portions of additional dengue virus serotype dimeric E proteins similarly produced.
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