利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.013 秒)

    Method for preparing a glycated hemoglobin solution
    3.
    发明授权
    Method for preparing a glycated hemoglobin solution 失效
    制备糖化血红蛋白溶液的方法

    公开(公告)号：US5589393A

    公开(公告)日：1996-12-31

    申请号：US427508

    申请日：1995-04-24

    申请人： Michael D. Fiechtner ,  John M. Ramp ,  Barbara J. England ,  Mary J. Annino

    发明人： Michael D. Fiechtner ,  John M. Ramp ,  Barbara J. England ,  Mary J. Annino

    IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/72 ,  G01N33/96 ,  G01N37/00

    CPC分类号： G01N33/723 ,  G01N33/54353 ,  G01N33/96 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/826 ,  Y10S436/827 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/25625

    摘要： The invention is a rapid, continuous test for glycated hemoglobin using a non-equilibrium affinity binding method. Agarose beads derivatized with 3-aminophenylboronic acid specifically bind glycated hemoglobin. This solid phase is incorporated into a sample processor card, modified to mix and to separate the test solution from the solid phase prior to absorbance readings. Two absorbance readings are made on the test solution, one immediately after mixing the reagent/diluent with the specimen, and one after a significant amount of binding has occurred. A linear correlation between total glycated hemoglobin and hemoglobin A.sub.1c permits standardization and reporting of units equivalent to % hemoglobin A.sub.1c. Stable glycated hemoglobin solutions for use as standards in the assay, and a method for preparing the standards are also disclosed.

    摘要翻译： 本发明是使用非平​​衡亲和力结合方法对糖化血红蛋白的快速连续测试。 用3-氨基苯基硼酸衍生的琼脂糖珠特异性结合糖化血红蛋白。 该固相被并入样品处理器卡中，在吸光度读数之前被修改以混合并将测试溶液与固相分离。 在测试溶液上进行两次吸光度读数，一次在试剂/稀释剂与样品混合后立即进行，并且发生了大量的结合之后。 总糖化血红蛋白和血红蛋白A1c之间的线性相关性允许标准化和报告相当于％血红蛋白A1c的单位。 还公开了用作测定中的标准物的稳定的糖化血红蛋白溶液和制备标准品的方法。

    Lead detection method and reggents utilizing aminolevulinic acid dehydratase and tertiary phosphines
    6.
    发明授权
    Lead detection method and reggents utilizing aminolevulinic acid dehydratase and tertiary phosphines 失效
    铅检测方法和利用氨基乙酰丙酸脱水酶和叔膦的方法

    公开(公告)号：US5552297A

    公开(公告)日：1996-09-03

    申请号：US419845

    申请日：1995-04-11

    申请人： Martin Wong ,  John M. Ramp ,  John M. Brackett

    发明人： Martin Wong ,  John M. Ramp ,  John M. Brackett

    IPC分类号： G01N33/52 ,  G01N33/84 ,  C12Q1/32 ,  G01N33/53

    CPC分类号： G01N33/84 ,  G01N33/52 ,  Y10S435/81

    摘要： A method and kit for simplifying and improving the sensitivity and accuracy of a lead assay for a sample solution suspected of containing lead determines the extent of a reaction between a substrate and a disulfide enzyme in the presence of an activating reagent which contains a water-soluble tertiary phosphine reagent so as to increase the activity of the disulfide enzyme for reaction with the substrate. For a colorimetric determination of the enzyme activity a chromophore is formed upon reaction with a selected component of the sample solution in the presence of a colorimetric enhancing reagent. The colorimetric enhancing reagent contains a metal ion such as cupric ion or ferric ion which is soluble in the sample solution. The extent of the chromophore formation is then photometrically determined.

    摘要翻译： 用于简化和提高疑似含有铅的样品溶液的铅测定的灵敏度和准确度的方法和试剂盒确定了在含有水溶性的活化试剂存在下底物和二硫化物酶之间的反应程度 以提高二硫化物酶与底物反应的活性。 为了比色测定酶活性，在比色增强剂的存在下，与样品溶液的选定组分反应形成发色团。 比色增强剂含有可溶于样品溶液的金属离子如铜离子或铁离子。 然后光度测定发色团形成的程度。