公开(公告)号：US4495293A

公开(公告)日：1985-01-22

申请号：US469323

申请日：1983-02-24

申请人： Mark R. Shaffar

发明人： Mark R. Shaffar

IPC分类号： C12Q1/00 ,  G01N33/52 ,  G01N33/542 ,  G01N33/58 ,  G01N21/17 ,  G01N21/25

CPC分类号： C12Q1/00 ,  G01N33/52 ,  G01N33/542 ,  G01N33/582 ,  Y10S436/80

摘要： A method is provided to fluorometrically determine a ligand in an assay solution containing the ligand, reagent system and a fluorescer wherein the intensity of the fluorescer emitted by the assay solution is related to the change in the transmittive properties of the assay solution produced by the interaction of the ligand to be determined and a reagent system capable of producing a change in the transmittive properties of the assay solution in the presence of the ligand. In addition, novel reagent compositions are provided which may be utilized to either spectrophotometrically or fluorometrically determine the concentration of a ligand in an assay solution.

摘要翻译： 提供了一种荧光测定方法来测定含有配体，试剂系统和荧光剂的测定溶液中的配体，其中由测定溶液发射的荧光剂的强度与由相互作用产生的测定溶液的透射性质的变化有关 的待配位的配体和能够在配体存在下产生测定溶液的透射性能变化的试剂体系。 此外，提供新的试剂组合物，其可用于分光光度法或荧光测定法测定测定溶液中配体的浓度。

公开(公告)号：US4743561A

公开(公告)日：1988-05-10

申请号：US708472

申请日：1985-03-05

申请人： Mark R. Shaffar

发明人： Mark R. Shaffar

IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12Q1/54 ,  G01N21/76 ,  G01N21/83 ,  G01N33/52 ,  G01N33/53 ,  G01N33/542 ,  C12Q1/66 ,  C12Q1/28

CPC分类号： C12Q1/00 ,  C12Q1/54 ,  G01N33/52 ,  Y10S436/805 ,  Y10S436/909 ,  Y10T436/144444 ,  Y10T436/146666 ,  Y10T436/147777 ,  Y10T436/166666 ,  Y10T436/171538 ,  Y10T436/19 ,  Y10T436/204165

摘要： An improved method and reagents are disclosed for determining a ligand in an assay solution containing the ligand, a reagent system and a luminescent compounds, wherein the intensity of the light emitted by the assay solution is related to the change in the transmittive properties of the assay solution produced by the interaction of the ligand to be determined and a reagent system capable of producing a change in the transmittive properties of the assay solution in the presence of the ligand.

摘要翻译： 公开了用于测定含有配体，试剂系统和发光化合物的测定溶液中的配体的改进方法和试剂，其中由测定溶液发射的光的强度与测定的透射性质的变化有关 通过待测配体的相互作用产生的溶液和能够在配体存在下产生测定溶液的透射性质变化的试剂体系。

公开(公告)号：US5597702A

公开(公告)日：1997-01-28

申请号：US350241

申请日：1994-12-09

申请人： Martin Wong ,  David M. Finley ,  John M. Ramp ,  Gary L. Boltinghouse, Jr. ,  Mark R. Shaffar ,  Stephen D. Stroupe ,  John M. Brackett

发明人： Martin Wong ,  David M. Finley ,  John M. Ramp ,  Gary L. Boltinghouse, Jr. ,  Mark R. Shaffar ,  Stephen D. Stroupe ,  John M. Brackett

IPC分类号： C12Q1/527 ,  C12Q1/52 ,  G01N21/78 ,  G01N31/00 ,  G01N33/48 ,  G01N33/52 ,  G01N33/84 ,  C12Q1/34 ,  G01N33/00

CPC分类号： G01N33/52 ,  C12Q1/52 ,  G01N33/84 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S514/836

摘要： Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

摘要翻译： 检测样品中存在的铅，包括以下步骤：（a）将铅回收剂加入到含有铅的测定溶液中; （b）向测定溶液中加入在铅存在下被抑制的二硫化物酶; 和（c）将二硫键的活性与样品中的铅的量相关联。 铅回收剂提高了测定的灵敏度和准确性，使得该测定可以容易地自动化，用于使用市售的自动化系统检测全血中的铅。

公开(公告)号：US4252896A

公开(公告)日：1981-02-24

申请号：US110429

申请日：1980-01-07

申请人： Mark R. Shaffar

发明人： Mark R. Shaffar

IPC分类号： A61K38/44 ,  C12N9/08 ,  C12N9/96 ,  C12Q1/00 ,  C12Q1/28 ,  G01N33/535 ,  C12Q1/66

CPC分类号： C12N9/0065 ,  C12N9/96 ,  C12Q1/28 ,  G01N33/535 ,  Y10S435/963

摘要： The addition of 8-anilino-1-napthalene sulfonic acid to a reagent comprising peroxidase in a serum protein based medium improves the stability of the enzyme 10-20 fold by blocking heme binding proteins and thereby maintaining the structural integrity of the enzyme.

摘要翻译： 在含有血清蛋白质的培养基中含有过氧化物酶的试剂中加入8-苯胺基-1-萘磺酸通过阻断血红素结合蛋白提高了10-20倍的酶的稳定性，从而保持酶的结构完整性。