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    Methods for the analysis of non-proteinaceous components using a protease from a Bacillus strain
    2.
    发明授权
    Methods for the analysis of non-proteinaceous components using a protease from a Bacillus strain 有权
    使用来自芽孢杆菌菌株的蛋白酶分析非蛋白质成分的方法

    公开(公告)号：US06727067B2

    公开(公告)日：2004-04-27

    申请号：US10007389

    申请日：2001-10-29

    申请人： Eberhard Russman ,  Thomas Meier ,  Ranier Schmuck ,  Johnny Staepels ,  Uwe Wehnes

    发明人： Eberhard Russman ,  Thomas Meier ,  Ranier Schmuck ,  Johnny Staepels ,  Uwe Wehnes

    IPC分类号： C12Q168

    CPC分类号： C12Q1/37 ,  C12N9/54 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Y304/21062 ,  G01N2333/32 ,  C12Q2521/537

    摘要： This invention relates to a method for the analysis of a (at least one) target non-proteinaceous component of a mixture of non-proteinaceous and proteinaceous components derived from a biological sample using a protease from a Bacillus strain. The invention further relates to a method for the analysis of a (at least one) target nucleic acid component of a mixture of non-proteinaceous components, which comprise nucleic acids, and proteinaceous components whereby the mixture is derived from a biological sample comprising the steps of incubating the mixture with a (at least one) protease from a Bacillus strain, optionally amplifying the (at least one) target nucleic acid component, and determining or detecting the (at least one) target nucleic acid component.

    摘要翻译： 本发明涉及使用来自芽孢杆菌菌株的蛋白酶从生物样品中分析非蛋白质和蛋白质成分的混合物的（至少一种）靶非蛋白质组分的方法。 本发明还涉及用于分析包含核酸和蛋白质组分的非蛋白质组分的混合物的（至少一种）靶核酸组分的方法，由此所述混合物衍生自包含步骤 将所述混合物与来自芽孢杆菌菌株的（至少一种）蛋白酶一起孵育，任选地扩增（至少一种）靶核酸组分，以及确定或检测（至少一种）靶核酸组分。

    Method for determining iron in a body fluid sample
    3.
    发明授权
    Method for determining iron in a body fluid sample 失效
    用于测定体液样品中铁的方法

    公开(公告)号：US4961970A

    公开(公告)日：1990-10-09

    申请号：US238620

    申请日：1988-08-30

    申请人： Joachim Siedel ,  Lieselotte Schellong ,  Johnny Staepels ,  Uwe Herrmann ,  Michael-Harold Town

    发明人： Joachim Siedel ,  Lieselotte Schellong ,  Johnny Staepels ,  Uwe Herrmann ,  Michael-Harold Town

    IPC分类号： G01N33/84

    CPC分类号： G01N33/84 ,  Y10S436/91

    摘要： The present invention provides a process for the determination of iron in body fluids by liberation of the bound iron, reduction to Fe.sup.2+, addition of a color system appropriate for the detection of iron and photometric measurement in a tenside-containing sample solution, wherein a fatty acid polyethylene glycol ester, an alkanol polyglycol ether and at least 1 mole/liter quanidine hydrochloride are added to the sample solution. The present invention also provides a reagent for the determination of iron in serum, containing a reducing agent, a color material system appropriate for the detection of iron and guanidine hydrochloride and at least one tenside, wherein it contains a fatty acid polyethylene glycol ester and a alkanol polyglycol ether.

    摘要翻译： 本发明提供了一种通过释放结合的铁，还原成Fe2 +，添加适合于检测铁的颜色系统和在含有表面活性剂的样品溶液中进行光度测量来测定体液中铁的方法，其中脂肪 酸性聚乙二醇酯，链烷醇聚乙二醇醚和至少1摩尔/升盐酸氢化物加入到样品溶液中。 本发明还提供了一种用于测定血清中含铁的试剂，其含有还原剂，适用于检测铁和胍盐酸盐和至少一种表面活性剂的着色材料体系，其中它含有脂肪酸聚乙二醇酯和 链烷醇聚乙二醇醚。