公开(公告)号：US20110306060A1

公开(公告)日：2011-12-15

申请号：US13139045

申请日：2009-12-02

Applicant: Yan Ni ,  Ayesha Sitlani ,  Shilpa Pandit ,  Dale Lewis ,  Xun Shen ,  Sharon Lobo ,  Timothy McCabe ,  Jon Condra ,  Rose Cubbon

Inventor： Yan Ni ,  Ayesha Sitlani ,  Shilpa Pandit ,  Dale Lewis ,  Xun Shen ,  Sharon Lobo ,  Timothy McCabe ,  Jon Condra ,  Rose Cubbon

IPC: G01N33/573

CPC classification number: C07K16/40 ,  C07K2317/55 ,  G01N33/543 ,  G01N33/573 ,  G01N2333/96433

Abstract: Methods of using PCSK9 antagonists More specifically, methods for measuring circulating PCSK9 levels in a bio-logical sample by means of an immunoassay The immunoassay used can be a solid phase immunoassay, such as a dissociation-enhanced lanthanide fluorescence immunoassay utilizing an E07 capture antibody or coating and a G08 or H23 detecting antibody.

Abstract translation: 使用PCSK9拮抗剂的方法更具体地，通过免疫测定法测量生物逻辑样品中的循环PCSK9水平的方法所用的免疫测定可以是固相免疫测定法，例如利用E07捕获抗体的解离增强镧系荧光免疫测定法 涂层和G08或H23检测抗体。

公开(公告)号：US08877900B2

公开(公告)日：2014-11-04

申请号：US13503729

申请日：2010-10-29

Applicant: Peter Peizhi Luo ,  Kevin Caili Wangr ,  Pingyu Zhong ,  Mark Hsieh ,  Yan Li ,  Xinwei Wang ,  Feng Dong ,  Andrei Golosov ,  Yan Ni ,  Weirong Wang ,  Laurence B. Peterson ,  Rose Cubbon ,  Sujata Sharma ,  Jon Condra ,  Jun Lu ,  Gopalakrishnan Parthasarathy ,  Stephen Soisson ,  Noel Byrne

Inventor： Peter Peizhi Luo ,  Kevin Caili Wangr ,  Pingyu Zhong ,  Mark Hsieh ,  Yan Li ,  Xinwei Wang ,  Feng Dong ,  Andrei Golosov ,  Yan Ni ,  Weirong Wang ,  Laurence B. Peterson ,  Rose Cubbon ,  Sujata Sharma ,  Jon Condra ,  Jun Lu ,  Gopalakrishnan Parthasarathy ,  Stephen Soisson ,  Noel Byrne

IPC: C07K16/40 ,  C07K16/00 ,  A61K39/395

CPC classification number: C07K16/40 ,  C07K2299/00 ,  C07K2317/21 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/00

Abstract: Antagonists of human proprotein convertase subtilisin-kexin type 9 (“PCSK9”) are disclosed. The disclosed antagonists are effective in the inhibition of PCSK9 function and, accordingly, present desirable antagonists for use in the treatment of conditions associated with PCSK9 activity. The present invention also discloses nucleic acid encoding said antagonists, vectors, host cells, and compositions comprising the antagonists. Methods of making PCSK9-specific antagonists as well as methods of using the antagonists for inhibiting or antagonizing PCSK9 function are also disclosed and form important additional aspects of the present disclosure.

Abstract translation: 公开了人前体蛋白转化酶枯草杆菌蛋白酶-Kexin 9型（“PCSK9”）的拮抗剂。 所公开的拮抗剂在抑制PCSK9功能方面是有效的，因此，呈现用于治疗与PCSK9活性相关的病症的所需拮抗剂。 本发明还公开了编码所述拮抗剂，载体，宿主细胞和包含拮抗剂的组合物的核酸。 还公开了制备PCSK9特异性拮抗剂的方法以及使用拮抗剂抑制或拮抗PCSK9功能的方法，并形成了本公开的重要的附加方面。

公开(公告)号：US20120219558A1

公开(公告)日：2012-08-30

申请号：US13497663

申请日：2010-09-15

Applicant: Yan Ni ,  Ayesha Sitlani ,  Shilpa Pandit ,  Dale Lewis ,  Xun Shen ,  Sharon Lobo ,  Timothy McCabe ,  Jon Condra ,  Rose Cubbon ,  Fubao Wang

Inventor： Yan Ni ,  Ayesha Sitlani ,  Shilpa Pandit ,  Dale Lewis ,  Xun Shen ,  Sharon Lobo ,  Timothy McCabe ,  Jon Condra ,  Rose Cubbon ,  Fubao Wang

IPC: A61K39/395 ,  C12N15/13 ,  C12N1/21 ,  C12N15/63 ,  C12P21/08 ,  C07K16/40 ,  A61P7/00

CPC classification number: C07K16/40 ,  C07K2317/76

Abstract: Antagonists of human proprotein convertase subtilisin-kexin type 9 (PCSK9) are disclosed. Said antagonists are effective in the inhibition of PCSK9 function and thereby provide compositions of matter useful for the treatment of conditions associated with PCSK9 activity. The present invention further discloses nucleic acids encoding PCSK9 antagonists as well as methods of making and using PCSK9 antagonists.

Abstract translation: 公开了人前体蛋白转化酶枯草杆菌蛋白酶-Kexin 9型（PCSK9）的拮抗剂。 所述拮抗剂在抑制PCSK9功能方面是有效的，从而提供可用于治疗与PCSK9活性相关的病症的物质组合物。 本发明还公开了编码PCSK9拮抗剂的核酸以及制备和使用PCSK9拮抗剂的方法。

公开(公告)号：US08748115B2

公开(公告)日：2014-06-10

申请号：US13139045

申请日：2009-12-02

Applicant: Yan Ni ,  Ayesha Sitlani ,  Shilpa Pandit ,  Dale Lewis ,  Xun Shen ,  Sharon Lobo ,  Timothy McCabe ,  Jon Condra ,  Rose Cubbon

Inventor： Yan Ni ,  Ayesha Sitlani ,  Shilpa Pandit ,  Dale Lewis ,  Xun Shen ,  Sharon Lobo ,  Timothy McCabe ,  Jon Condra ,  Rose Cubbon

IPC: G01N33/573 ,  G01N33/543 ,  C07K16/40

CPC classification number: C07K16/40 ,  C07K2317/55 ,  G01N33/543 ,  G01N33/573 ,  G01N2333/96433

Abstract: Methods of using PCSK9 antagonists More specifically, methods for measuring circulating PCSK9 levels in a biological sample by means of an immunoassay The immunoassay used can be a solid phase immunoassay, such as a dissociation-enhanced lanthanide fluorescence immunoassay utilizing an E07 capture antibody or coating and a G08 or H23 detecting antibody.

Abstract translation: 使用PCSK9拮抗剂的方法更具体地，通过免疫测定法测量生物样品中的循环PCSK9水平的方法使用的免疫测定法可以是固相免疫测定法，例如利用E07捕获抗体或涂层的解离增强镧系荧光免疫测定法 G08或H23检测抗体。

公开(公告)号：US20120213794A1

公开(公告)日：2012-08-23

申请号：US13503729

申请日：2010-10-29

Applicant: Peter Peizhi Luo ,  Kevin Caili Wangr ,  Pingyu Zhong ,  Mark Hsieh ,  Yan Li ,  Xinwei Wang ,  Feng Dong ,  Andrei Golosov ,  Yan Ni ,  Weirong Wang ,  Laurence B. Peterson ,  Rose Cubbon ,  Sujata Sharma ,  Jon Condra ,  Jun Lu ,  Gopalakrishnan Parthasarathy ,  Stephen Soisson ,  Noel Byrne

Inventor： Peter Peizhi Luo ,  Kevin Caili Wangr ,  Pingyu Zhong ,  Mark Hsieh ,  Yan Li ,  Xinwei Wang ,  Feng Dong ,  Andrei Golosov ,  Yan Ni ,  Weirong Wang ,  Laurence B. Peterson ,  Rose Cubbon ,  Sujata Sharma ,  Jon Condra ,  Jun Lu ,  Gopalakrishnan Parthasarathy ,  Stephen Soisson ,  Noel Byrne

IPC: A61K39/395 ,  C12N15/13 ,  C12P21/02 ,  A61P3/06 ,  C12N5/10 ,  C12N1/19 ,  C12N1/21 ,  C07K16/40 ,  C12N15/63

CPC classification number: C07K16/40 ,  C07K2299/00 ,  C07K2317/21 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/00

Abstract: Antagonists of human proprotein convertase subtilisin-kexin type 9 (“PCSK9”) are disclosed. The disclosed antagonists are effective in the inhibition of PCSK9 function and, accordingly, present desirable antagonists for use in the treatment of conditions associated with PCSK9 activity. The present invention also discloses nucleic acid encoding said antagonists, vectors, host cells, and compositions comprising the antagonists. Methods of making PCSK9-specific antagonists as well as methods of using the antagonists for inhibiting or antagonizing PCSK9 function are also disclosed and form important additional aspects of the present disclosure.

Abstract translation: 公开了人前体蛋白转化酶枯草杆菌蛋白酶-Kexin 9型（“PCSK9”）的拮抗剂。 所公开的拮抗剂在抑制PCSK9功能方面是有效的，因此，呈现用于治疗与PCSK9活性相关的病症的所需拮抗剂。 本发明还公开了编码所述拮抗剂，载体，宿主细胞和包含拮抗剂的组合物的核酸。 还公开了制备PCSK9特异性拮抗剂的方法以及使用拮抗剂抑制或拮抗PCSK9功能的方法，并形成了本公开的重要的附加方面。