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公开(公告)号：US20100331206A1
公开(公告)日：2010-12-30
申请号：US12825223
申请日：2010-06-28
IPC分类号： C40B30/04 , A61J1/00 , G01N33/53 , C07K16/18 , C12N5/16 , C12P21/04 , C12Q1/68 , C07K14/47 , C07H21/04
CPC分类号： G01N33/689 , C07K14/47 , G01N2333/471 , G01N2800/368 , Y10S435/973
摘要： Pregnancy-associated glycoproteins (PAGs) are structurally related to the pepsins, thought to be restricted to the hoofed (ungulate) mammals and characterized by being expressed specifically in the outer epithelial cell layer (chorion/trophectoderm) of the placenta. By cloning expressed genes from ovine and bovine placental cDNA libraries, the inventors estimate that cattle, sheep, and most probably all ruminant Artiodactyla, possess possibly 100 or more PAG genes, many of which are placentally expressed. The PAGs are highly diverse in sequence, with regions of hypervariability confined largely to surface-exposed loops. Selected PAG that are products of the invasive binucleate cells, expressed highly in early pregnancy at the time of trophoblast invasion and expressed weakly, if at all, in late gestation are useful in the early diagnosis of pregnancy. In a preferred embodiment, the invention relates to immunoassays for detecting these PAGs.
摘要翻译： 妊娠相关糖蛋白（PAG）在结构上与胃蛋白酶相关，被认为限于蹄（有蹄类）的哺乳动物，其特征在于在胎盘的外上皮细胞层（绒毛膜/滋养外胚层）中特异性表达。 通过克隆来自绵羊和牛胎盘cDNA文库的表达基因，本发明人估计，牛，绵羊和大多数所有反刍动物偶蹄类动物都具有100个或更多个PAG基因，其中许多是胎盘表达的。 PAG的序列高度多样化，高变区域主要局限于表面暴露的环路。 选择的PAG是侵入性双核细胞的产物，在滋养层侵袭时早期表达高度表达，如果在妊娠晚期，在妊娠早期诊断中很有用。 在优选的实施方案中，本发明涉及用于检测这些PAG的免疫测定。
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公开(公告)号：US07763432B2
公开(公告)日：2010-07-27
申请号：US11552454
申请日：2006-10-24
IPC分类号： G01N33/53
CPC分类号： G01N33/689 , C07K14/47 , G01N2333/471 , G01N2800/368 , Y10S435/973
摘要： Pregnancy-associated glycoproteins (PAGs) are structurally related to the pepsins, thought to be restricted to the hoofed (ungulate) mammals and characterized by being expressed specifically in the outer epithelial cell layer (chorion/trophectoderm) of the placenta. By cloning expressed genes from ovine and bovine placental cDNA libraries, the inventors estimate that cattle, sheep, and most probably all ruminant Artiodactyla, possess possibly 100 or more PAG genes, many of which are placentally expressed. The PAGs are highly diverse in sequence, with regions of hypervariability confined largely to surface-exposed loops. Selected PAG that are products of the invasive binucleate cells, expressed highly in early pregnancy at the time of trophoblast invasion and expressed weakly, if at all, in late gestation are useful in the early diagnosis of pregnancy. In a preferred embodiment, the invention relates to immunoassays for detecting these PAGs.
摘要翻译： 妊娠相关糖蛋白（PAG）在结构上与胃蛋白酶相关，被认为限于蹄（有蹄类）的哺乳动物，其特征在于在胎盘的外上皮细胞层（绒毛膜/滋养外胚层）中特异性表达。 通过克隆来自绵羊和牛胎盘cDNA文库的表达基因，本发明人估计，牛，绵羊和大多数所有反刍动物偶蹄类动物都具有100个或更多个PAG基因，其中许多是胎盘表达的。 PAG的序列高度多样化，高变区域主要局限于表面暴露的环路。 选择的PAG是侵入性双核细胞的产物，在滋养层侵袭时早期表达高度表达，如果在妊娠晚期，在妊娠早期诊断中很有用。 在优选的实施方案中，本发明涉及用于检测这些PAG的免疫测定。
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公开(公告)号：US06869770B1
公开(公告)日：2005-03-22
申请号：US09273164
申请日：1999-03-19
CPC分类号： G01N33/689 , C07K14/47 , G01N2333/471 , G01N2800/368 , Y10S435/973
摘要： Pregnancy-associated glycoproteins (PAGs) are structurally related to the pepsins, thought to be restricted to the hoofed (ungulate) mammals and characterized by being expressed specifically in the outer epithelial cell layer (chorion/trophectoderm) of the placenta. By cloning expressed genes from ovine and bovine placental cDNA libraries, the inventors estimate that cattle, sheep, and most probably all ruminant Artiodactyla, possess possibly 100 or more PAG genes, many of which are placentally expressed. The PAGs are highly diverse in sequence, with regions of hypervariability confined largely to surface-exposed loops. Selected PAG that are products of the iOnvasive binucleate cells, expressed highly in early pregnancy at the time of trophoblast invasion and expressed weakly, if at all, in late gestation are useful in the early diagnosis of pregnancy. In a preferred embodiment, the invention relates to immunoassays for detecting these PAGs.
摘要翻译： 妊娠相关糖蛋白（PAG）在结构上与胃蛋白酶相关，被认为限于蹄（有蹄类）的哺乳动物，其特征在于在胎盘的外上皮细胞层（绒毛膜/滋养外胚层）中特异性表达。 通过克隆来自绵羊和牛胎盘cDNA文库的表达基因，本发明人估计，牛，绵羊和大多数所有反刍动物偶蹄类动物都具有100个或更多个PAG基因，其中许多是胎盘表达的。 PAG的序列高度多样化，高变区域主要局限于表面暴露的环路。 选择的PAG是iOnvasive双核细胞的产物，在滋养层侵袭时早期表达高度表达，在妊娠晚期如果有的话在早期诊断怀孕中有用。 在优选的实施方案中，本发明涉及用于检测这些PAG的免疫测定。
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公开(公告)号：US08182999B2
公开(公告)日：2012-05-22
申请号：US12825223
申请日：2010-06-28
IPC分类号： G01N33/53
CPC分类号： G01N33/689 , C07K14/47 , G01N2333/471 , G01N2800/368 , Y10S435/973
摘要： Pregnancy-associated glycoproteins (PAGs) are structurally related to the pepsins, thought to be restricted to the hoofed (ungulate) mammals and characterized by being expressed specifically in the outer epithelial cell layer (chorion/trophectoderm) of the placenta. By cloning expressed genes from ovine and bovine placental cDNA libraries, the inventors estimate that cattle, sheep, and most probably all ruminant Artiodactyla, possess possibly 100 or more PAG genes, many of which are placentally expressed. The PAGs are highly diverse in sequence, with regions of hypervariability confined largely to surface-exposed loops. Selected PAG that are products of the invasive binucleate cells, expressed highly in early pregnancy at the time of trophoblast invasion and expressed weakly, if at all, in late gestation are useful in the early diagnosis of pregnancy. In a preferred embodiment, the invention relates to immunoassays for detecting these PAGs.
摘要翻译： 妊娠相关糖蛋白（PAG）在结构上与胃蛋白酶相关，被认为限于蹄（有蹄类）的哺乳动物，其特征在于在胎盘的外上皮细胞层（绒毛膜/滋养外胚层）中特异性表达。 通过克隆来自绵羊和牛胎盘cDNA文库的表达基因，本发明人估计，牛，绵羊和大多数所有反刍动物偶蹄类动物都具有100个或更多个PAG基因，其中许多是胎盘表达的。 PAG的序列高度多样化，高变区域主要局限于表面暴露的环路。 选择的PAG是侵入性双核细胞的产物，在滋养层侵袭时早期表达高度表达，如果在妊娠晚期，在妊娠早期诊断中很有用。 在优选的实施方案中，本发明涉及用于检测这些PAG的免疫测定。
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