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    SINGLE MOLECULE MASS OR SIZE SPECTROMETRY IN SOLUTION USING A SOLITARY NANOPORE
    1.
    发明申请
    SINGLE MOLECULE MASS OR SIZE SPECTROMETRY IN SOLUTION USING A SOLITARY NANOPORE 审中-公开
    单分子单分子质谱或尺寸分光光度计

    公开(公告)号：US20100122907A1

    公开(公告)日：2010-05-20

    申请号：US12435667

    申请日：2009-05-05

    申请人： Vincent M. Stanford ,  John J. Kasianowicz ,  Joseph W.F. Robertson ,  Claudio G. Rodrigues ,  Oleg V. Krasilnikov

    发明人： Vincent M. Stanford ,  John J. Kasianowicz ,  Joseph W.F. Robertson ,  Claudio G. Rodrigues ,  Oleg V. Krasilnikov

    IPC分类号： G01N27/447

    CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  C12Q2537/165 ,  C12Q2563/116 ,  C12Q2565/631

    摘要： A nanopore conductance measurement method and system is provided. The system has reservoirs of conductive fluid separated by a resistive barrier, which is perforated by a single nanometer scale pore commensurate in size with an analyte molecule in at least one of the reservoirs. The system is configured to have an ionic current driven across the reservoirs by an applied potential and the pore may be treated so that the pore surface can form associations with the analyte molecules of interest to increase the analyte molecule residence times on or in the pore. The system also comprises a means of measuring the ionic current, which current may be either direct or alternating in time, induced by an applied potential between electrodes in the conductive fluid, on each side of the barrier. The system also comprises a means of recording the ionic current time course as a time series, which includes time periods when the pore is unobstructed and also in periods when analyte molecules cause pulses of reduced conductance. The method comprises methods to delineate segments of the conductance time series into regions statistically consistent with the unobstructed pore conductance level, and pulses of reduced conductance, and also statistically stationary segments within individual pulses of reduced conductance. The method may also provide steps for interpreting the statistical analysis to yield parameters such as size, mass, and/or concentration of at least one type of analyte in solution.

    摘要翻译： 提供纳米孔电导测量方法和系统。 该系统具有由电阻屏障分隔的导电流体的储存器，该电阻屏障通过与至少一个储存器中的分析物分子大小相当的单个纳米尺度孔穿孔。 该系统被配置为具有通过施加的电势驱动跨越储存器的离子电流，并且可以处理孔以使孔表面可以与感兴趣的分析物分子形成关联，以增加分析物分子在孔上或孔中的停留时间。 该系统还包括测量离子电流的手段，该电流可以是在屏障的每一侧上的导电流体中的电极之间的施加电位引起的时间上的直接或交替的电流。 该系统还包括将离子电流时间过程记录为时间序列的手段，时间序列包括当孔隙畅通的时间段以及分析物分子引起电导率降低的时段。 该方法包括将电导时间序列的段划分到与无阻碍的孔电导水平统计学一致的区域，以及降低的电导的脉冲以及降低的电导的单个脉冲内的统计学上固定的区段的方法。 该方法还可以提供解释统计分析以产生诸如解决方案中的至少一种类型的分析物的尺寸，质量和/或浓度的参数的步骤。