公开(公告)号：US20190309008A1

公开(公告)日：2019-10-10

申请号：US16371646

申请日：2019-04-01

申请人： Jingyue JU ,  Shiv KUMAR ,  Chuanjuan TAO ,  Minchen CHIEN ,  James J. RUSSO ,  John J. KASIANOWICZ ,  Joseph W.F. ROBERTSON

发明人： Jingyue JU ,  Shiv KUMAR ,  Chuanjuan TAO ,  Minchen CHIEN ,  James J. RUSSO ,  John J. KASIANOWICZ ,  Joseph W.F. ROBERTSON

IPC分类号： C07H19/00 ,  C12Q1/48 ,  C12Q1/42 ,  C07H19/16 ,  C12Q1/6869 ,  C07H19/06 ,  G01N33/487 ,  C12Q1/6883

摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

公开(公告)号：US20160024570A1

公开(公告)日：2016-01-28

申请号：US14775451

申请日：2014-03-14

申请人： Jingyue JU ,  Shiv KUMAR ,  Mirko PALLA ,  James J. RUSSO

发明人： Jingyue JU ,  Shiv KUMAR ,  Mirkó PALLA ,  James J. RUSSO

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  G01N21/65 ,  G01N21/658 ,  C12Q2535/122 ,  C12Q2561/113 ,  C12Q2565/632

摘要： This invention provides nucleoside polyphosphate analogs each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs; and methods for determining the sequence of a single-stranded DNA or RNA using said nucleoside polyphosphate analogs. This invention also provides methods for detecting the interaction of a plurality of predetermined compounds, at least one of which having attached thereto a tag comprising a plurality of Raman-scattering moieties.

摘要翻译： 本发明提供核苷多磷酸酯类似物，其各自包含包含多个拉曼散射部分的标签; 包含所述核苷多磷酸酯类似物的化合物; 以及使用所述核苷多磷酸酯类似物测定单链DNA或RNA的序列的方法。 本发明还提供了用于检测多个预定化合物的相互作用的方法，其中至少一个化合物与其附着有包含多个拉曼散射部分的标签。