公开(公告)号：US20110123572A1

公开(公告)日：2011-05-26

申请号：US12377585

申请日：2007-08-16

申请人： Jozef Bizik ,  Ari Harjula ,  Esko Kankuri ,  Antti Vaheri

发明人： Jozef Bizik ,  Ari Harjula ,  Esko Kankuri ,  Antti Vaheri

IPC分类号： A61K9/00 ,  A61K35/12 ,  A61K38/18 ,  A61K38/19 ,  C12N5/071 ,  A61P17/02 ,  A61P43/00 ,  A61P9/10 ,  A61P9/00

CPC分类号： A61K35/33 ,  A61K35/12 ,  C12N5/0656 ,  C12N2501/12

摘要： The invention provides activated fibroblasts for the treatment of tissue and/or organ damage in a patient. Fibroblasts are activated by culturing the cells under conditions that induce the cells to adhere to each other and simultaneously to secrete growth factors, especially hepatocyte growth factor, HGF. The invention also provides a pharmaceutical composition based on the medium in which the activated fibroblasts are cultured. The invention further provides methods for transplantation.

摘要翻译： 本发明提供用于治疗患者组织和/或器官损伤的活化成纤维细胞。 通过在诱导细胞彼此粘附并同时分泌生长因子，特别是肝细胞生长因子HGF的条件下培养细胞来激活成纤维细胞。 本发明还提供了基于培养活化成纤维细胞的培养基的药物组合物。 本发明还提供了用于移植的方法。

公开(公告)号：US20090298171A1

公开(公告)日：2009-12-03

申请号：US12295599

申请日：2007-03-30

申请人： Jozef Bizik ,  Ari Lasse Juhani Harjula ,  Esko Markus Kankuri

发明人： Jozef Bizik ,  Ari Lasse Juhani Harjula ,  Esko Markus Kankuri

IPC分类号： C12N5/06 ,  C12M1/34

CPC分类号： C12N5/0694 ,  C12N2502/1323

摘要： The present invention is directed to a method of treating cells by co-culturing them with activated fibroblasts in order to regulate the growth and/or status of the cells. Fibroblasts are activated by culturing the cells under conditions that induce the cells to adhere to each other to form multicellular aggregates or spheroids. The present invention also provides a device for selecting cells from cell samples, such as bone marrow aspirate, the device comprises said multicellular aggregates.

摘要翻译： 本发明涉及通过与活化的成纤维细胞共培养来处理细胞的方法，以调节细胞的生长和/或状态。 通过在诱导细胞彼此粘附以形成多细胞聚集体或球体的条件下培养细胞来活化成纤维细胞。 本发明还提供了用于从细胞样品中选择细胞的装置，例如骨髓抽吸物，该装置包括所述多细胞聚集体。