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    Glucose sensor
    1.
    发明授权
    Glucose sensor 有权
    葡萄糖传感器

    公开(公告)号：US06773564B1

    公开(公告)日：2004-08-10

    申请号：US09406832

    申请日：1999-09-28

    申请人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata ,  Shoji Miyazaki ,  Hideyuki Baba ,  Seiji Takeshima

    发明人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata ,  Shoji Miyazaki ,  Hideyuki Baba ,  Seiji Takeshima

    IPC分类号： G01N27327

    CPC分类号： C12Q1/006

    摘要： The present invention provides a high performance glucose sensor which can demonstrate high stability against preservation and produces only a low blank value. The glucose sensor comprises an electrically insulating base plate, an electrode system formed on the base plate, and a reaction layer which is formed in contact with or in the vicinity of the electrode system and contains at least a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the reaction layer further containing an additive such as phthalic acid.

    摘要翻译： 本发明提供了一种高性能葡萄糖传感器，其可以显示对保存的高稳定性并且仅产生低的空白值。 葡萄糖传感器包括电绝缘基板，形成在基板上的电极系统和与电极系统接触或附近形成的反应层，并且至少含有辅酶是吡咯并喹啉的葡萄糖脱氢酶 醌，反应层还含有邻苯二甲酸等添加剂。

    Cholesterol sensor containing electrodes, cholesterol dehydrogenase, nicotinamide adenine dinucleotide and oxidized electron mediator
    2.
    发明授权
    Cholesterol sensor containing electrodes, cholesterol dehydrogenase, nicotinamide adenine dinucleotide and oxidized electron mediator 失效
    胆固醇传感器含有电极，胆固醇脱氢酶，烟酰胺腺嘌呤二核苷酸和氧化电子介体

    公开(公告)号：US06214612B1

    公开(公告)日：2001-04-10

    申请号：US08806801

    申请日：1997-03-03

    申请人： Tomohiro Yamamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata

    发明人： Tomohiro Yamamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata

    IPC分类号： C12M134

    CPC分类号： C12Q1/005 ,  Y10S435/817

    摘要： A cholesterol sensor for quantitative determination of cholesterol is provided containing an electrode system and a reaction reagent system. The electrode system contains a measuring electrode such as a carbon electrode and a counter electrode, and the reaction reagent system contains cholesterol dehydrogenase, nicotinamide adenine dinucleotide and an oxidized electron mediator. Electron mediators include ferricyanide, 1,2-naphthoquinone-4-sulfonate, 2,6-dichlorophenol indophenol, dimethylbenzoquinone, 1-methoxy-5-methylphenazinium sulfate, methylene blue, gallocyanine, thionine, phenazine methosulfate and Meldola's blue. Diaphorase, cholesterol esterase and a surfactant may also be present. The electrode system is on an insulating base plate, and the base plate has a covering member containing a groove that is a sample supplying channel which extends from an end of the base plate to the electrode system. A reaction layer containing the reagent system in dry form and a layer of a hydrophilic polymer is provided on the base plate or the covering member, or on both the electrode system and covering member so as to be exposed to the sample supplying channel. During operation, the electron mediator is reduced in conjunction with oxidation of cholesterol in a sample by cholesterol dehydrogenase, and an amount of current required to electrochemically re-oxidize the electron mediator is directly proportional to a quantity of cholesterol present in the sample.

    摘要翻译： 提供了用于定量测定胆固醇的胆固醇传感器，其包含电极系统和反应试剂系统。 电极系统含有碳电极和对电极等测定电极，反应试剂系统含有胆固醇脱氢酶，烟酰胺腺嘌呤二核苷酸和氧化电子介体。 电子介体包括铁氰化物，1,2-萘醌-4-磺酸盐，2,6-二氯苯酚靛酚，二甲基苯并醌，1-甲氧基-5-甲基吩嗪鎓硫酸盐，亚甲基蓝，花青素，硫堇，吩嗪甲硫酸盐和梅尔多拉蓝。 也可以存在心律变性酶，胆固醇酯酶和表面活性剂。 电极系统在绝缘基板上，基板具有包含作为从基板的端部延伸到电极系统的样品供给路径的槽的覆盖部件。 含有干式试剂体系和亲水性聚合物层的反应层设置在基板或覆盖部件上，或电极系统和覆盖部件上，以暴露于样品供应通道。 在操作期间，电子介体与胆固醇脱氢酶在样品中的胆固醇氧化一起被还原，并且电化学再氧化电子介体所需的电流量与样品中存在的胆固醇量成正比。