公开(公告)号：US06547954B2

公开(公告)日：2003-04-15

申请号：US09232320

申请日：1999-01-18

申请人： Shin Ikeda ,  Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Shigeki Joko ,  Junko Iwata

发明人： Shin Ikeda ,  Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Shigeki Joko ,  Junko Iwata

IPC分类号： G01N27327

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A biosensor includes: an insulating substrate; an electrode system formed on the insulating substrate which has a working electrode and a counter electrode; and a reaction layer formed on the insulating substrate which contains an oxidoreductase and an electron acceptor. The electron acceptor is ferricinium ion derived from ferrocene electrolyte.

摘要翻译： 生物传感器包括：绝缘基板; 形成在具有工作电极和对电极的绝缘基板上的电极系统; 以及形成在包含氧化还原酶和电子受体的绝缘基板上的反应层。 电子受体是衍生自二茂铁电解质的三价铁离子。

公开(公告)号：US5922188A

公开(公告)日：1999-07-13

申请号：US812602

申请日：1997-03-07

申请人： Shin Ikeda ,  Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Shigeki Joko ,  Junko Iwata

发明人： Shin Ikeda ,  Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Shigeki Joko ,  Junko Iwata

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A biosensor includes: an insulating substrate; an electrode system formed on the insulating substrate which has a working electrode and a counter electrode; and a reaction layer formed on the insulating substrate which contains an oxidoreductase and an electron acceptor. The electron acceptor is ferricinium ion derived from ferrocene electrolyte.

公开(公告)号：US06214612B1

公开(公告)日：2001-04-10

申请号：US08806801

申请日：1997-03-03

申请人： Tomohiro Yamamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata

发明人： Tomohiro Yamamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata

IPC分类号： C12M134

CPC分类号： C12Q1/005 ,  Y10S435/817

摘要： A cholesterol sensor for quantitative determination of cholesterol is provided containing an electrode system and a reaction reagent system. The electrode system contains a measuring electrode such as a carbon electrode and a counter electrode, and the reaction reagent system contains cholesterol dehydrogenase, nicotinamide adenine dinucleotide and an oxidized electron mediator. Electron mediators include ferricyanide, 1,2-naphthoquinone-4-sulfonate, 2,6-dichlorophenol indophenol, dimethylbenzoquinone, 1-methoxy-5-methylphenazinium sulfate, methylene blue, gallocyanine, thionine, phenazine methosulfate and Meldola's blue. Diaphorase, cholesterol esterase and a surfactant may also be present. The electrode system is on an insulating base plate, and the base plate has a covering member containing a groove that is a sample supplying channel which extends from an end of the base plate to the electrode system. A reaction layer containing the reagent system in dry form and a layer of a hydrophilic polymer is provided on the base plate or the covering member, or on both the electrode system and covering member so as to be exposed to the sample supplying channel. During operation, the electron mediator is reduced in conjunction with oxidation of cholesterol in a sample by cholesterol dehydrogenase, and an amount of current required to electrochemically re-oxidize the electron mediator is directly proportional to a quantity of cholesterol present in the sample.

摘要翻译： 提供了用于定量测定胆固醇的胆固醇传感器，其包含电极系统和反应试剂系统。 电极系统含有碳电极和对电极等测定电极，反应试剂系统含有胆固醇脱氢酶，烟酰胺腺嘌呤二核苷酸和氧化电子介体。 电子介体包括铁氰化物，1,2-萘醌-4-磺酸盐，2,6-二氯苯酚靛酚，二甲基苯并醌，1-甲氧基-5-甲基吩嗪鎓硫酸盐，亚甲基蓝，花青素，硫堇，吩嗪甲硫酸盐和梅尔多拉蓝。 也可以存在心律变性酶，胆固醇酯酶和表面活性剂。 电极系统在绝缘基板上，基板具有包含作为从基板的端部延伸到电极系统的样品供给路径的槽的覆盖部件。 含有干式试剂体系和亲水性聚合物层的反应层设置在基板或覆盖部件上，或电极系统和覆盖部件上，以暴露于样品供应通道。 在操作期间，电子介体与胆固醇脱氢酶在样品中的胆固醇氧化一起被还原，并且电化学再氧化电子介体所需的电流量与样品中存在的胆固醇量成正比。

公开(公告)号：US20050072670A1

公开(公告)日：2005-04-07

申请号：US10478283

申请日：2002-12-27

申请人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  C12Q1/60 ,  G01N27/403 ,  G01N33/487 ,  G01N33/49 ,  G01N27/26

CPC分类号： C12Q1/001 ,  C12Q1/006 ,  C12Q1/60 ,  G01N27/3272

摘要： In order to provide a biosensor with high accuracy and excellent response where plasma obtained by filtration of blood rapidly arrives at an electrode system, in a biosensor comprising: an insulating base plate; an electrode system having a working electrode and a counter electrode which are provided on the base plate; a reaction layer including at least oxidoreductase and an electron mediator; a sample solution supply pathway which includes the electrode system and the reaction layer and has an inlet and an air aperture; a sample solution supply part for introducing a sample solution, which is in position apart from the sample solution supply pathway; and a first filter which is disposed between the sample solution supply pathway and the sample solution supply part for filtering the sample solution, where the filtrate filtered with the first filter is supplied into the sample solution supply pathway due to capillary action, the direction in which the sample solution passes through the first filter and the direction in which the filtrate passes through the sample solution supply pathway are made cross at right angles.

摘要翻译： 为了提供具有高精度和优异响应的生物传感器，其中通过过滤血液获得的血液迅速到达电极系统，在生物传感器中包括：绝缘基板; 具有设置在基板上的工作电极和对置电极的电极系统; 至少包含氧化还原酶和电子介体的反应层; 包括电极系统和反应层并具有入口和空气孔的样品溶液供应路径; 用于引入样品溶液的样品溶液供应部件，其处于离样品溶液供给路径的位置; 以及第一过滤器，其设置在样品溶液供给路径和样品溶液供给部之间，用于过滤样品溶液，其中用第一过滤器过滤的滤液由于毛细作用而被供给到样品溶液供给通道中，其中 样品溶液通过第一过滤器，滤液通过样品溶液供应通道的方向被成直角交叉。

公开(公告)号：US06706232B2

公开(公告)日：2004-03-16

申请号：US10363589

申请日：2003-03-04

申请人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27327

CPC分类号： C12Q1/001

摘要： There is provided a cholesterol sensor with high-accuracy and excellent response, whose object to be measured is whole blood, where plasma with hemocytes therein filtered can rapidly reach an electrode system. In a biosensor where plasma with hemocytes therein filtered with a filter is sucked into a sample solution supply pathway due to capillarity, there are formed: a first pressing part for holding a primary side portion of the filter from the bottom; a second pressing part for holding a secondary side portion of the filter from the top and the bottom; a third pressing part for holding the central portion of the filter from the top; and a void for surrounding the filter between the second pressing part and third pressing part.

摘要翻译： 提供了具有高精度和优异反应的胆固醇传感器，其被测量对象是全血，其中血细胞在其中被过滤的血浆可以快速地达到电极系统。 在生物传感器中，其中用过滤器过滤血细胞的血浆由于毛细作用而被吸入样品溶液供应通道中，形成有：从底部保持过滤器的初级侧部分的第一按压部分; 用于从顶部和底部保持过滤器的次级侧部分的第二按压部分; 用于从顶部保持过滤器的中心部分的第三按压部分; 以及用于围绕第二按压部和第三按压部之间的过滤器的空隙。

公开(公告)号：USRE39390E1

公开(公告)日：2006-11-14

申请号：US10924334

申请日：2002-05-17

申请人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Miwa Hasegawa ,  Tomohiro Yamamoto ,  Motokazu Watanabe ,  Takahiro Nakaminami ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327

摘要： There is provided a cholesterol sensor with high-accuracy and excellent response, whose object to be measured is whole blood, where plasma with which is obtained by filtering out hemocytes therein filtered in blood can rapidly reach an electrode system. In a biosensor where plasma with which is obtained by filtering out hemocytes therein filtered with by a filter is sucked into a sample solution supply pathway due to capillarity, there are formed: a first pressing part for holding a primary side portion of the filter from the bottom; a second pressing part for holding a secondary side portion of the filter from the top and the bottom; a third pressing part for holding the central portion of the filter from the top; and a void for surrounding the filter between the second pressing part and third pressing part.

摘要翻译： 提供了具有高精度和优异反应的胆固醇传感器，其测量对象为全血，等离子体<？delete-start id =“DEL-S-00001”date =“20061114”？>与<？delete -end id =“DEL-S-00001”？> <？insert-start id =“INS-S-00001”date =“20061114”？>通过过滤出<？insert-end id =“INS- S-00001“？>血细胞<？delete-start id =”DEL-S-00002“date =”20061114“？>其中过滤的<？delete-end id =”DEL-S-00002“？> 在血液中开始id =“INS-S-00002”date =“20061114”？> <？insert-end id =“INS-S-00002”？>可以快速到达电极系统。 在等离子体<？delete-start id =“DEL-S-00003”date =“20061114”？>与<？delete-end id =“DEL-S-00003”？> <？insert-start id = 通过滤出<？insert-end id =“INS-S-00003”？>血细胞<？delete-start id =“DEL-S-00004”获得的“INS-S-00003”date =“20061114”？ “date =”20061114“？>用<？delete-end id =”DEL-S-00004“？> <？insert-start id =”INS-S-00004“date =”20061114“？> ？insert-end id =“INS-S-00004”？>由于毛细作用，将过滤器吸入样品溶液供给路径，形成有：从底部保持过滤器的初级侧部的第一按压部， 用于从顶部和底部保持过滤器的次级侧部分的第二按压部分; 用于从顶部保持过滤器的中心部分的第三按压部分; 以及用于围绕第二按压部和第三按压部之间的过滤器的空隙。

公开(公告)号：US06773564B1

公开(公告)日：2004-08-10

申请号：US09406832

申请日：1999-09-28

申请人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata ,  Shoji Miyazaki ,  Hideyuki Baba ,  Seiji Takeshima

发明人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata ,  Shoji Miyazaki ,  Hideyuki Baba ,  Seiji Takeshima

IPC分类号： G01N27327

CPC分类号： C12Q1/006

摘要： The present invention provides a high performance glucose sensor which can demonstrate high stability against preservation and produces only a low blank value. The glucose sensor comprises an electrically insulating base plate, an electrode system formed on the base plate, and a reaction layer which is formed in contact with or in the vicinity of the electrode system and contains at least a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the reaction layer further containing an additive such as phthalic acid.

摘要翻译： 本发明提供了一种高性能葡萄糖传感器，其可以显示对保存的高稳定性并且仅产生低的空白值。 葡萄糖传感器包括电绝缘基板，形成在基板上的电极系统和与电极系统接触或附近形成的反应层，并且至少含有辅酶是吡咯并喹啉的葡萄糖脱氢酶 醌，反应层还含有邻苯二甲酸等添加剂。

公开(公告)号：US5759445A

公开(公告)日：1998-06-02

申请号：US641765

申请日：1996-05-02

申请人： Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N33/92 ,  G01N33/96 ,  B01J13/00 ,  C12Q1/60

CPC分类号： G01N33/96 ,  G01N33/92 ,  G01N2800/044 ,  Y10S516/928 ,  Y10T436/104165

摘要： There is disclosed an aqueous dispersed solution as a standard solution for determining lipid levels, having a dispersion form and a particle size, which are similar to those of serum lipids. A lipid-dispersed solution is obtained by evaporating an organic solvent from a mixture prepared by adding cholesterol, a phospholipid, a bile acid or bile acid salt, and a neutral lipid and/or a cholesterol ester in the organic solvent, swelling the resultant thin-film like mixture with water or buffer heated at a temperature higher than a phase transition temperature of the lecithin, and then dispersing the solution by a physical shear force. There can be easily obtained a solution having a cholesterol concentration of about less than 1,000 mg/dl, which is extremely stable and can be stored for a long period of time.

摘要翻译： 公开了水分散溶液作为用于测定脂质水平的标准溶液，具有与血清脂质相似的分散体形式和粒径。 通过将有机溶剂从通过在有机溶剂中加入胆固醇，磷脂，胆汁酸或胆汁酸盐和中性脂质和/或胆固醇酯制备的混合物蒸发而获得脂质分散溶液，将所得薄片 在与高于卵磷脂相变温度的温度下加热的水或缓冲液混合，然后通过物理剪切力分散溶液。 可以容易地获得胆固醇浓度大约小于1,000mg / dl的溶液，其极为稳定并且可以长期储存。

公开(公告)号：US6071392A

公开(公告)日：2000-06-06

申请号：US89350

申请日：1998-06-03

申请人： Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/005 ,  Y10S435/817

摘要： A cholesterol sensor is disclosed which comprises an electrode system having a measuring electrode and a counter electrode formed on an electrically insulating base plate, an electrode coating layer for covering the electrode system and a reaction reagent layer formed on or in the vicinity of the electrode coating layer, wherein the reaction reagent layer comprises at least an enzyme for catalyzing cholesterol oxidation, an enzyme having a cholesterol ester hydrolyzing activity and a surfactant, the electrode coating layer comprises at least one selected from the group consisting of water-soluble cellulose derivatives and saccharides and is contained at such a concentration that imparts sufficient viscosity to a sample solution for enabling it to hinder invasion of said surfactant into said electrode system when said electrode coating layer is dissolved in said sample solution supplied to said sensor. This configuration best eliminates impairment of sensor response due to electrode degeneration caused by invading surfactant into the electrode system.

摘要翻译： 公开了一种胆固醇传感器，其包括具有测量电极和形成在电绝缘基板上的对电极的电极系统，用于覆盖电极系统的电极涂层和形成在电极涂层上或附近的反应试剂层 其中所述反应试剂层至少包含用于催化胆固醇氧化的酶，具有胆固醇酯水解活性的酶和表面活性剂，所述电极涂层包含选自水溶性纤维素衍生物和糖类中的至少一种 并且以使得当样品溶液溶解在提供给所述传感器的所述样品溶液中时能使其阻止所述表面活性剂侵入所述电极系统的浓度给予足够的粘度。 该配置最好地消除由于将表面活性剂侵入电极系统引起的电极变性的传感器响应的损害。

公开(公告)号：US06225078B1

公开(公告)日：2001-05-01

申请号：US09122591

申请日：1998-07-27

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q126

CPC分类号： C12Q1/004 ,  C12Q1/006

摘要： The invention provides a method for quantitative measurement of a substrate with high accuracy by electrochemically oxidizing an electron mediator which has been reduced by enzyme reaction thereby determining the substrate concentration based on a current flowing during electrochemical oxidation from which adverse effects of an easy-to-oxidize substance on the oxidation process have been minimized. The quantitating method in accordance with the present invention comprises a first step for causing a substrate contained in a sample to react with a specific oxidoreductase to the substrate in the presence of an electron mediator in oxidized state, and a second step for electrochemically reducing the electron mediator in oxidized state which remains non-reduced by the enzyme reaction in the first step, thereby obtaining a current flowing during electrochemical reduction.

摘要翻译： 本发明提供了一种用于通过电化学氧化已经通过酶反应降低的电子介体来高精度地定量测量衬底的方法，从而基于在电化学氧化期间流动的电流来确定衬底浓度，从而容易地 - 在氧化过程中氧化物质已被最小化。 根据本发明的定量方法包括第一步骤，用于在电子介体的氧化态存在下使包含在样品中的底物与特定的氧化还原酶与底物反应，第二步用于电化学还原电子 在第一步中通过酶反应保持未还原的氧化态介体，从而获得在电化学还原过程中流动的电流。