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    Methods And Compositions For Cellular Imaging And Cancer Cell Detection Using Light Harvesting Conjugated Polymer-Biomolecular Conjugates
    3.
    发明申请
    Methods And Compositions For Cellular Imaging And Cancer Cell Detection Using Light Harvesting Conjugated Polymer-Biomolecular Conjugates 审中-公开
    细胞成像的方法和组合和使用光收获共轭聚合物 - 生物分子结合体的癌细胞检测

    公开(公告)号：US20130109029A1

    公开(公告)日：2013-05-02

    申请号：US13806447

    申请日：2011-06-29

    申请人： Bin Liu ,  Kanyi Pu ,  Kai Li ,  Liping Cai ,  Yanyan Wang ,  Dan Ding

    发明人： Bin Liu ,  Kanyi Pu ,  Kai Li ,  Liping Cai ,  Yanyan Wang ,  Dan Ding

    IPC分类号： C07K17/02

    CPC分类号： C07K17/02 ,  B82Y15/00 ,  C07D285/10 ,  C09K11/06 ,  C09K2211/1416 ,  G01N33/582 ,  G01N33/588

    摘要： The present invention relates to conjugated polyelectrolyte (CPE) or oligoelectrolyte (COE) compounds represented by general structural formulae (I)-(IV), or a salt thereof and methods of using these compounds to detect targets in samples. In particular, the methods include: (1) exposing a sample to a compound of structural formula (I), (II) or (IV) or a salt thereof, allowing the compound to bind to a target and detecting a signal produced by the compound; (2) functionalizing a solid support with a ligand, incubating the sample with a charged CPE or COE and detecting the fluorescence of the solid support and thereby detecting the target or (3) functionalizing a surface of a solid support with a charged ligand, thereby creating a charge on the surface of the solid support; incubating the ligand-functionalized solid support with a sample, whereupon binding of the target, the charge on the surface of the solid support switches; incubating the sample with CPE or COE that has a complementary charge to the charge of the target-bound surface; and detecting the fluorescence of the solid support and thereby detecting the target. The compounds of the present invention possess high photoluminescence quantum yields in biological media, low cytotoxicity, and excellent environmental stability and photostability and can be used in biosensor and bioimaging applications.

    摘要翻译： 本发明涉及由通式（I） - （IV）表示的共轭聚电解质（CPE）或低聚电解质（COE）化合物或其盐，以及使用这些化合物检测样品中靶标的方法。 特别地，所述方法包括：（1）将样品暴露于结构式（I），（II）或（IV）的化合物或其盐，使化合物与靶标结合并检测由 复合; （2）用配体官能化固体支持物，将样品与带电荷的CPE或COE孵育并检测固体支持物的荧光，从而检测靶，或（3）用带电的配体使固体支持物的表面官能化，从而 在固体支撑体的表面上产生电荷; 将配体官能化的固体支持物与样品一起孵育，随后靶标结合，固体支持开关表面上的电荷; 将样品与具有补充电荷的CPE或COE孵育到靶结合表面的电荷; 并检测固体支持物的荧光，从而检测目标物。 本发明的化合物在生物培养基中具有高的光致发光量子产率，低的细胞毒性和优异的环境稳定性和光稳定性，并可用于生物传感器和生物成像应用。