公开(公告)号：US07405036B2

公开(公告)日：2008-07-29

申请号：US10900061

申请日：2004-07-26

申请人： Naida M. Loskutoff ,  Kari Morfeld ,  James Dow

发明人： Naida M. Loskutoff ,  Kari Morfeld ,  James Dow

IPC分类号： A01N1/02 ,  C12N5/00 ,  C07G15/00

CPC分类号： A61L2/0011 ,  Y10T436/25125 ,  Y10T436/25375

摘要： The present invention is directed towards an apparatus and method for disinfecting biological samples, including semen and the like, and for removing a biological sample pellet from surrounding media while reducing the risk of recontamination of the pellet with said media. An insert receptacle includes a receiving cylinder having a drain in a floor which allows the fluids to decant slowly down the side of a tube. The insert receptacle further includes an aspiration channel which allows an aspiration device, such as a modified micro-pipette, to be utilized for extracting a sample pellet from the bottom of the tube. Utilizing the novel apparatus, a method for reducing pathogens in a biological sample includes decanting the biological sample through the receiving cylinder and into a tube where it passes through a multi-layer gradient including an enzyme suitable for removal of at least one pathogen from the biological sample. Centrifuging of the insert receptacle, tube, and sample, isolates a sample pellet of the biological sample from the surrounding at least one pathogen. After the sample pellet is isolated it is aspirated via the aspiration device without re-contaminating the sample pellet through contact with the surrounding at least one pathogen.

摘要翻译： 本发明涉及用于消毒生物样品（包括精液等）的装置和方法，以及用于从周围介质中除去生物样品沉淀物，同时降低与所述培养基重新沉淀的风险。 插入容器包括容纳圆筒，底座中有排水管，允许流体沿管的侧面缓慢倾斜。 插入容器还包括抽吸通道，其允许诸如改进的微量移液管的抽吸装置用于从管的底部提取样品颗粒。 利用该新颖的装置，用于减少生物样品中的病原体的方法包括将生物样品通过接收圆筒倾倒并进入其中通过多层梯度的管中，所述多层梯度包括适于从生物中去除至少一种病原体的酶 样品。 插入容器，管和样品的离心分离生物样品的样品颗粒与周围的至少一种病原体。 在分离样品颗粒后，通过抽吸装置吸出样品沉淀，而不会通过与周围的至少一种病原体接触而再次污染样品颗粒。

公开(公告)号：US20050064579A1

公开(公告)日：2005-03-24

申请号：US10900061

申请日：2004-07-26

申请人： Naida Loskutoff ,  Kari Morfeld ,  James Dow

发明人： Naida Loskutoff ,  Kari Morfeld ,  James Dow

IPC分类号： A61L2/00 ,  C12M1/00

CPC分类号： A61L2/0011 ,  Y10T436/25125 ,  Y10T436/25375

摘要： The present invention is directed towards an apparatus and method for disinfecting biological samples, including semen and the like, and for removing a biological sample pellet from surrounding media while reducing the risk of recontamination of the pellet with said media. An insert receptacle includes a receiving cylinder having a drain in a floor which allows the fluids to decant slowly down the side of a tube. The insert receptacle further includes an aspiration channel which allows an aspiration device, such as a modified micro-pipette, to be utilized for extracting a sample pellet from the bottom of the tube. Utilizing the novel apparatus, a method for reducing pathogens in a biological sample includes decanting the biological sample through the receiving cylinder and into a tube where it passes through a multi-layer gradient including an enzyme suitable for removal of at least one pathogen from the biological sample. Centrifuging of the insert receptacle, tube, and sample, isolates a sample pellet of the biological sample from the surrounding at least one pathogen. After the sample pellet is isolated it is aspirated via the aspiration device without re-contaminating the sample pellet through contact with the surrounding at least one pathogen.

摘要翻译： 本发明涉及用于消毒生物样品（包括精液等）的装置和方法，并且用于从周围介质中除去生物样品沉淀物，同时降低与所述培养基重新沉淀的风险。 插入容器包括容纳圆筒，底座中有排水管，允许流体沿管的侧面缓慢倾斜。 插入容器还包括抽吸通道，其允许诸如改进的微量移液管的抽吸装置用于从管的底部提取样品颗粒。 利用该新颖的装置，用于减少生物样品中的病原体的方法包括将生物样品通过接收圆筒倾倒并进入其中通过多层梯度的管中，所述多层梯度包括适于从生物中去除至少一种病原体的酶 样品。 插入容器，管和样品的离心分离生物样品的样品颗粒与周围的至少一种病原体。 在分离样品颗粒后，通过抽吸装置吸出样品沉淀，而不会通过与周围的至少一种病原体接触而再次污染样品颗粒。