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    Method for the genus-specific or/and species-specific detection of bacteria in a sample liquid
    2.
    发明授权
    Method for the genus-specific or/and species-specific detection of bacteria in a sample liquid 失效
    用于样品液体中细菌的特异性或/和物种特异性检测的方法

    公开(公告)号：US06225094B1

    公开(公告)日：2001-05-01

    申请号：US08073985

    申请日：1993-06-08

    申请人： Wolfgang Ludwig ,  Karl-Heinz Schleifer ,  Christoph Kessler ,  Ruediger Rueger ,  Anne Stern

    发明人： Wolfgang Ludwig ,  Karl-Heinz Schleifer ,  Christoph Kessler ,  Ruediger Rueger ,  Anne Stern

    IPC分类号： C12P1934

    CPC分类号： C12Q1/689 ,  C12Q1/6858

    摘要： For the genus-specific or/and species-specific detection of bacteria in a sample liquid, bacterial RNA is hybridized with a primer which is complementary to a genus-specific or species-specific region of the RNA of particular bacteria or to a highly conserved region of the RNA of bacteria in general, but which is not complementary at its 3′ end to the RNA of bacteria of another genus or species, the primer is elongated in the presence of a suitable polymerase and the four deoxyribonucleotides, if desired, with a concurrent or subsequent labelling of the elongation product and an elongation product formed is hybridized with a genus-specific or species-specific oligonucleotide after denaturation and the hybridization is detected by means of the oligonucleotide label.

    摘要翻译： 对于样品液体中细菌的特异性或/和物种特异性检测，细菌RNA与与特定细菌的RNA的特异性或物种特异性区域互补的引物杂交，或与高度保守的 一般细菌的RNA的区域，但是在其3'末端与另一属或物种的细菌的RNA不互补，则引物在合适的聚合酶存在下延长，如果需要，四种脱氧核糖核苷酸与 延伸产物的同时或随后的标记和形成的延伸产物与变性后的属特异性或物种特异性寡核苷酸杂交，并通过寡核苷酸标记检测杂交。