公开(公告)号：US20120210451A1

公开(公告)日：2012-08-16

申请号：US13388535

申请日：2010-08-02

申请人： Hirofumi Shimizu ,  Kazuo Ohashi ,  Rie Utoh ,  Kazuya Ise ,  Masayuki Yamato ,  Teruo Okano ,  Mitsukazu Gotoh

发明人： Hirofumi Shimizu ,  Kazuo Ohashi ,  Rie Utoh ,  Kazuya Ise ,  Masayuki Yamato ,  Teruo Okano ,  Mitsukazu Gotoh

IPC分类号： A61K35/39 ,  C12N5/071 ,  A61P1/18 ,  G01N21/64 ,  A61P3/10 ,  A61K9/00 ,  A01K67/027

CPC分类号： A61L27/3804 ,  A61L27/3604 ,  A61L27/38 ,  C12N5/0676 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2539/10

摘要： A polymer that changes hydration at a temperature between 0 to 80° C. is coated on the surface of a cell culture support, and islet cells are cultured on the support at a temperature range that causes polymer to have weak hydration, then the temperature of a culture solution is changed to a temperature that causes the polymer to have strong hydration to obtain islet cells in a sheet form. Such islet cells in a sheet form have an insulin producing function even if there is no blood flow.

摘要翻译： 将在0至80℃温度下变化水合的聚合物涂覆在细胞培养载体的表面上，并且在使得聚合物具有弱水合的温度范围内将胰岛细胞在载体上培养，然后将温度 将培养溶液变为导致聚合物具有强水合作用以获得片状的胰岛细胞的温度。 即使没有血液流动，片状的这种胰岛细胞也具有产生胰岛素的功能。