公开(公告)号：US5426042A

公开(公告)日：1995-06-20

申请号：US179593

申请日：1994-01-07

申请人： Shigeru Maeda ,  Akira Ohki ,  Takeshi Sato ,  Naho Kato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Keizo Hatagaki ,  Yasushi Takahashi ,  Mikio Yamada ,  Hajime Okumura

发明人： Shigeru Maeda ,  Akira Ohki ,  Takeshi Sato ,  Naho Kato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Keizo Hatagaki ,  Yasushi Takahashi ,  Mikio Yamada ,  Hajime Okumura

IPC分类号： C12Q1/00 ,  G01N33/18 ,  C12N11/10 ,  C12N1/12 ,  C12N11/04 ,  G01N27/26

CPC分类号： G01N33/1806 ,  C12Q1/001 ,  G01N33/1866 ,  G01N33/1893 ,  Y10S435/817 ,  Y10S435/852

摘要： A BOD analyzer is prepared having a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in the membrane. Specifically, the BOD analyzer has a flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane, and a liquid passage which is connected with an entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from alginic acid or salts thereof, agar, gellan gum, xathane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, or pullulan. The BOD analyzer can be used for batch or continuous BOD analysis and enables carrying out BOD analysis in a short period of time.

摘要翻译： 制备具有含有氧电极和微生物膜的微生物传感器的BOD分析仪。 微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。 具体而言，BOD分析装置具有配置有含有氧电极和微生物膜的微生物传感器的流通池，与流通池的入口连接并具有出口的液体通道。 通过使用至少一种选自藻酸或其盐，琼脂，结冷胶的凝胶剂，将属于克雷伯氏菌12092菌株的微生物直接固定在平均孔径为0.65-3μm的多孔亲水膜中来制备微生物膜 口香糖，xathane胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶或支链淀粉。 BOD分析仪可用于批量或连续BOD分析，可在短时间内进行BOD分析。