公开(公告)号：US5413690A

公开(公告)日：1995-05-09

申请号：US97331

申请日：1993-07-23

申请人： Kent M. Kost ,  Thomas J. Lindsay ,  John F. Price

发明人： Kent M. Kost ,  Thomas J. Lindsay ,  John F. Price

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/416 ,  G01N27/26

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A potentiometric biosensor, test strip, reagent and a potentiometric method for detection or measurement of an analyte from a fluid sample. The biosensor includes indicating and reference electrodes, and a reagent, which overlays a portion of the surface of the indicating electrode. The reagent generally includes an enzyme and a redox mediator. When a fluid sample (e.g., blood) containing an analyte (e.g., glucose) is added to the reagent, the enzyme (e.g., glucose oxidase) catalyzes a reaction involving enzyme, redox mediator (e.g., potassium ferricyanide), and analyte.This reaction may be monitored by monitoring the electrical potential at the indicating electrode surface. (Electrical potential at the indicating electrode surface changes as the concentration of oxidized and reduced forms of the redox mediator change at the indicating electrode surface.) The inventive potentiometric biosensor measures the change in potential at the indicating electrode surface after the fluid sample containing the analyte is added to the reagent. The change in potential that occurs may be correlated to the detection or measurement of analyte in the fluid sample.

摘要翻译： 用于从流体样品中检测或测量分析物的电位生物传感器，试纸条，试剂和电位法。 生物传感器包括指示电极和参考电极以及覆盖指示电极表面的一部分的试剂。 试剂通常包括酶和氧化还原介体。 当将含有分析物（例如葡萄糖）的流体样品（例如血液）加入到试剂中时，酶（例如，葡萄糖氧化酶）催化涉及酶，氧化还原介体（例如铁氰化钾）和分析物的反应。 可以通过监测指示电极表面的电位来监测该反应。 （指示电极表面的电位随着氧化还原介体的氧化还原形式的浓度在指示电极表面变化而变化。）本发明的电位生物传感器测量含有分析物的流体样品之后的指示电极表面的电位变化 加入到试剂中。 发生的电位变化可能与流体样品中分析物的检测或测量相关。

公开(公告)号：US5508171A

公开(公告)日：1996-04-16

申请号：US198407

申请日：1994-02-22

申请人： P. Douglas Walling ,  Joseph E. Bateson ,  Lance S. Kuhn ,  M. Luann Ochs ,  Kent M. Kost ,  Martin T. Gerber ,  Klaus H. Pollmann ,  Chi-Neng A. Han

发明人： P. Douglas Walling ,  Joseph E. Bateson ,  Lance S. Kuhn ,  M. Luann Ochs ,  Kent M. Kost ,  Martin T. Gerber ,  Klaus H. Pollmann ,  Chi-Neng A. Han

IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12Q1/54

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A new electrochemical biosensor and method of its use. The electrochemical biosensor has a pair of electrodes consisting of a working electrode and a counter electrode made of the same electrically conducting materials. The counter electrode may be the same size or smaller than the working electrode. The biosensor includes a sample receiving portion wherein a portion of the electrode surfaces is covered by a reagent that includes a redox mediator and an enzyme. A fluid that contains an analyte is added to the sample receiving portion. A reaction involving the analyte, enzyme and redox mediator occurs. After this reaction is complete, an electrical potential difference is applied between the electrodes. Diffusion limited current is measured and correlated to the concentration of analyte in the fluid.

摘要翻译： 一种新的电化学生物传感器及其使用方法。 电化学生物传感器具有由工作电极和由相同导电材料制成的对电极组成的一对电极。 对电极的尺寸可以与工作电极相同。 生物传感器包括样品接收部分，其中一部分电极表面被包含氧化还原介体和酶的试剂覆盖。 含有分析物的流体被添加到样品接收部分。 发生涉及分析物，酶和氧化还原介体的反应。 在该反应完成之后，在电极之间施加电位差。 测量扩散限制电流并与流体中分析物的浓度相关。