公开(公告)号：US5508171A

公开(公告)日：1996-04-16

申请号：US198407

申请日：1994-02-22

申请人： P. Douglas Walling ,  Joseph E. Bateson ,  Lance S. Kuhn ,  M. Luann Ochs ,  Kent M. Kost ,  Martin T. Gerber ,  Klaus H. Pollmann ,  Chi-Neng A. Han

发明人： P. Douglas Walling ,  Joseph E. Bateson ,  Lance S. Kuhn ,  M. Luann Ochs ,  Kent M. Kost ,  Martin T. Gerber ,  Klaus H. Pollmann ,  Chi-Neng A. Han

IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12Q1/54

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A new electrochemical biosensor and method of its use. The electrochemical biosensor has a pair of electrodes consisting of a working electrode and a counter electrode made of the same electrically conducting materials. The counter electrode may be the same size or smaller than the working electrode. The biosensor includes a sample receiving portion wherein a portion of the electrode surfaces is covered by a reagent that includes a redox mediator and an enzyme. A fluid that contains an analyte is added to the sample receiving portion. A reaction involving the analyte, enzyme and redox mediator occurs. After this reaction is complete, an electrical potential difference is applied between the electrodes. Diffusion limited current is measured and correlated to the concentration of analyte in the fluid.

摘要翻译： 一种新的电化学生物传感器及其使用方法。 电化学生物传感器具有由工作电极和由相同导电材料制成的对电极组成的一对电极。 对电极的尺寸可以与工作电极相同。 生物传感器包括样品接收部分，其中一部分电极表面被包含氧化还原介体和酶的试剂覆盖。 含有分析物的流体被添加到样品接收部分。 发生涉及分析物，酶和氧化还原介体的反应。 在该反应完成之后，在电极之间施加电位差。 测量扩散限制电流并与流体中分析物的浓度相关。

公开(公告)号：US5288636A

公开(公告)日：1994-02-22

申请号：US627667

申请日：1990-12-14

申请人： Klaus H. Pollmann ,  Martin T. Gerber

发明人： Klaus H. Pollmann ,  Martin T. Gerber

IPC分类号： C12M1/34 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/54 ,  G01N27/28 ,  G01N27/327 ,  G01N27/416 ,  C12M1/40

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A biosensor that includes working and counter electrodes of substantially the same size and made of the same electrically conducting material. A reagent is contained in a reagent well in the biosensor. The reagent includes a redox mediator, an enzyme, and a buffer, and covers substantially equal surface areas of portions of the working and counter electrodes. When a sample, containing the analyte to be measured, is added to the reagent well, a reaction occurs that oxidizes the analyte and reduces the redox mediator. After this reaction is complete, an electrical potential difference is applied between the electrodes. The amount of oxidized form of the redox mediator at the counter electrode and the applied potential difference must be sufficient to cause diffusion limited electrooxidation of the reduced form of the redox mediator at the surface of the working electrode. After a short time delay, current produced by the electrooxidation of the reduced form of the redox mediator is measured and the observed current is correlated to the amount of the analyte in the sample. In some cases, the analyte sought to be measured may be reduced and the redox mediator may be oxidized. In such cases, current produced by diffusion limited electroreduction of the oxidized form of the redox mediator is measured and correlated to the amount of analyte in the sample.

摘要翻译： 一种生物传感器，其包括基本上相同尺寸并由相同导电材料制成的工作电极和对电极。 试剂在生物传感器的试剂孔中。 试剂包括氧化还原介体，酶和缓冲剂，并且覆盖工作电极和对电极的部分的基本上相等的表面积。 当将含有要测量的分析物的样品加入到试剂孔中时，发生氧化分析物并还原氧化还原介体的反应。 在该反应完成之后，在电极之间施加电位差。 在对电极处的氧化还原介体的氧化形式的量和施加的电位差​​必须足以在工作电极的表面上引起还原形式的氧化还原介体的扩散限制的电氧化。 在短时间延迟之后，测量通过还原形式的氧化还原介体的电氧化产生的电流，并且观察到的电流与样品中分析物的量相关。 在某些情况下，寻求测量的分析物可能被减少，氧化还原介体可被氧化。 在这种情况下，测量由氧化还原介体的氧化形式的扩散限制的电还原产生的电流，并与样品中分析物的量相关。