公开(公告)号：US5527897A

公开(公告)日：1996-06-18

申请号：US151391

申请日：1993-11-12

申请人： Kinichiro Oda ,  Susumu Nakada ,  Eiji Hara ,  Tomoko Yamaguchi ,  Takeshi Nakamura ,  Yumiko Oka ,  Toshihiko Kishimoto

发明人： Kinichiro Oda ,  Susumu Nakada ,  Eiji Hara ,  Tomoko Yamaguchi ,  Takeshi Nakamura ,  Yumiko Oka ,  Toshihiko Kishimoto

IPC分类号： A61K31/70 ,  A61K38/00 ,  C07H21/04 ,  C07K14/00 ,  C07K14/47 ,  C12N1/21 ,  C12N15/09 ,  C12N15/12 ,  C12Q1/68 ,  C12R1/19 ,  C12N15/00

CPC分类号： C07K14/4703 ,  A61K38/00

摘要： Disclosed herein are Human Id genes (Id-1H and Id-1H') and variants thereof. Origin, preparation process, biochemical functions and the like: two synthetic oligonucleotides separately having base sequences corresponding to regions of amino acid sequence, which are almost completely conserved by three mouse Id genes (Id-1, Id-2 and HLH462) are used as probes to screen clones having respective cDNAs complementary to the probes from a cDNA library of human TIG-1 fibroblasts in accordance with the hybridization method, thereby isolating human Id genes. The expression of the human Id genes specifically varies according to the state of cell proliferation, in particular, the acquisition of cell aging and permanently proliferating ability.Expression vectors with the human Id gene or variant thereof integrated therein, transformed cell strains with the expression vector introduced therein, diagnostic probes obtained by chemically synthesizing a partial sequence of the base sequence of the human Id gene, or a variation sequence thereof, and antisense oligonucleotides composed of a base sequences complementary to the partial sequence of the base sequence of the human Id gene, or the variation sequence thereof are also disclosed.

摘要翻译： 本文公开了人Id基因（Id-1H和Id-1H'）及其变体。 原产地，制备方法，生物化学功能等：分别具有对应于三个小鼠Id基因（Id-1，Id-2和HLH462）几乎完全保守的氨基酸序列区域的碱基序列的两个合成寡核苷酸被用作 探针根据杂交方法筛选具有与人TIG-1成纤维细胞的cDNA文库互补的各cDNA的克隆，从而分离人Id基因。 人Id基因的表达特异性地根据细胞增殖的状态而变化，特别是获得细胞衰老和永久增殖能力。 其中整合了人Id基因或其变体的表达载体，其中引入了表达载体的转化细胞株，通过化学合成人Id基因的碱基序列的部分序列或其变异序列获得的诊断探针，以及反义 还公开了由与人Id基因的碱基序列的部分序列互补的碱基序列或其变异序列组成的寡核苷酸。