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    Modified epimorphin
    1.
    发明授权
    Modified epimorphin 失效
    修饰的异丙肾上腺素

    公开(公告)号：US6127149A

    公开(公告)日：2000-10-03

    申请号：US493071

    申请日：1995-06-21

    申请人： Yohei Hirai ,  Shogo Koshida ,  Yumiko Oka

    发明人： Yohei Hirai ,  Shogo Koshida ,  Yumiko Oka

    IPC分类号： C07K14/475 ,  C12N15/12 ,  C12N15/18 ,  C07K14/435 ,  C12N5/00 ,  C12N15/63

    CPC分类号： C07K14/475

    摘要： Disclosed herein are a modified epimorphin obtained by adding a hydrophilic peptide composed of 5 to 99 amino acids to at least one terminus of a polypeptide containing the functional domain of epimorphin, and a modified epimorphin composed of a polypeptide having a structure wherein a hydrophobic domain adjacent to the C-terminus of the whole-length epimorphin consisting of a coiled coil domain (1) on the N-terminal side, a functional domain (2) at the center, a coiled coil domain (3) on the C-terminal side and the hydrophobic domain (4) adjacent to the C-terminus has been deleted from the whole-length epimorphin, and at least part of amino acids have been deleted from the terminal side of at least one of the coiled coil domains (1) and (3) as well. The invention also discloses a variant modified epimorphin obtained by making partial substitution, deletion and/or insertion of amino acids in the amino acid sequence of the modified epimorphin, wherein the variant maintains the function of the original sequence. The invention further discloses DNAs encoding the modified epimorphin and variant thereof, recombinant vectors containing the DNAs, transformants obtained by introducing the recombinant vectors, and a production method of the modified epimorphin and the variants thereof making use of the transformant.

    摘要翻译： 本文公开了通过将由5至99个氨基酸组成的亲水肽添加至含有异丙肾上腺素的功能结构域的多肽的至少一个末端而获得的修饰的异激肽，以及由具有以下结构的多肽组成的经修饰的异胶原：其中疏水结构域相邻 到由N-末端侧的线圈圈（1），中心的功能域（2），C端侧的线圈区域（3）构成的全长异末端肽的C-末端 并且邻近C末端的疏水结构域（4）已经从全长异叶氨酸缺失，并且至少部分氨基酸已经从至少一个卷曲线圈结构域（1）和 （3）。 本发明还公开了通过在修饰的epimorphin的氨基酸序列中进行氨基酸的部分取代，缺失和/或插入获得的变体修饰的epimorphin，其中变体维持原始序列的功能。 本发明进一步公开了编码修饰的epimorphin及其变体的DNA，含有DNA的重组载体，通过引入重组载体获得的转化体，以及使用该转化体的修饰的epimorphin及其变体的制备方法。

    Oligopeptides for promoting hair growth
    2.
    发明授权
    Oligopeptides for promoting hair growth 失效
    促进头发生长的寡肽

    公开(公告)号：US07241731B2

    公开(公告)日：2007-07-10

    申请号：US10155922

    申请日：2002-05-23

    申请人： Yohei Hirai ,  Yumiko Oka ,  Kyoko Takebe ,  Hokari Tsuda ,  Keiko Tochigi ,  Toko Shinagawa ,  Kayoko Murakami ,  Shogo Koshida

    发明人： Yohei Hirai ,  Yumiko Oka ,  Kyoko Takebe ,  Hokari Tsuda ,  Keiko Tochigi ,  Toko Shinagawa ,  Kayoko Murakami ,  Shogo Koshida

    IPC分类号： A61K31/00

    CPC分类号： A61K38/08 ,  C07K14/475 ,  G01N2800/20

    摘要： The present invention provides oligopeptides having morphogenesis promoting activity and in particular, hair promoting activity. The oligopeptides may be in monomer form, monomer having a reactive substance bound form or as a polymer, such as a dimer including a homodimer; heterodimer; homotrimer; or heterodimer. The present invention also provides monoclonal antibodies that specifically recognize a 220 kDa antigen of epithelial new hair follicles; hybridomas producing such antibody; and methods and kits for assaying hair growth in mammalian subjects.

    摘要翻译： 本发明提供具有形态发生促进活性的特异性促发活性的寡肽。 寡肽可以是单体形式，具有反应性物质结合形式的单体或作为聚合物，例如包含同二聚体的二聚体; 异二聚体 同三聚体 或异二聚体。 本发明还提供特异性识别上皮新毛囊的220kDa抗原的单克隆抗体; 产生这种抗体的杂交瘤; 以及用于测定哺乳动物受试者毛发生长的方法和试剂盒。

    Human ID genes
    3.
    发明授权
    Human ID genes 失效
    人类基因

    公开(公告)号：US5527897A

    公开(公告)日：1996-06-18

    申请号：US151391

    申请日：1993-11-12

    申请人： Kinichiro Oda ,  Susumu Nakada ,  Eiji Hara ,  Tomoko Yamaguchi ,  Takeshi Nakamura ,  Yumiko Oka ,  Toshihiko Kishimoto

    发明人： Kinichiro Oda ,  Susumu Nakada ,  Eiji Hara ,  Tomoko Yamaguchi ,  Takeshi Nakamura ,  Yumiko Oka ,  Toshihiko Kishimoto

    IPC分类号： A61K31/70 ,  A61K38/00 ,  C07H21/04 ,  C07K14/00 ,  C07K14/47 ,  C12N1/21 ,  C12N15/09 ,  C12N15/12 ,  C12Q1/68 ,  C12R1/19 ,  C12N15/00

    CPC分类号： C07K14/4703 ,  A61K38/00

    摘要： Disclosed herein are Human Id genes (Id-1H and Id-1H') and variants thereof. Origin, preparation process, biochemical functions and the like: two synthetic oligonucleotides separately having base sequences corresponding to regions of amino acid sequence, which are almost completely conserved by three mouse Id genes (Id-1, Id-2 and HLH462) are used as probes to screen clones having respective cDNAs complementary to the probes from a cDNA library of human TIG-1 fibroblasts in accordance with the hybridization method, thereby isolating human Id genes. The expression of the human Id genes specifically varies according to the state of cell proliferation, in particular, the acquisition of cell aging and permanently proliferating ability.Expression vectors with the human Id gene or variant thereof integrated therein, transformed cell strains with the expression vector introduced therein, diagnostic probes obtained by chemically synthesizing a partial sequence of the base sequence of the human Id gene, or a variation sequence thereof, and antisense oligonucleotides composed of a base sequences complementary to the partial sequence of the base sequence of the human Id gene, or the variation sequence thereof are also disclosed.

    摘要翻译： 本文公开了人Id基因（Id-1H和Id-1H'）及其变体。 原产地，制备方法，生物化学功能等：分别具有对应于三个小鼠Id基因（Id-1，Id-2和HLH462）几乎完全保守的氨基酸序列区域的碱基序列的两个合成寡核苷酸被用作 探针根据杂交方法筛选具有与人TIG-1成纤维细胞的cDNA文库互补的各cDNA的克隆，从而分离人Id基因。 人Id基因的表达特异性地根据细胞增殖的状态而变化，特别是获得细胞衰老和永久增殖能力。 其中整合了人Id基因或其变体的表达载体，其中引入了表达载体的转化细胞株，通过化学合成人Id基因的碱基序列的部分序列或其变异序列获得的诊断探针，以及反义 还公开了由与人Id基因的碱基序列的部分序列互补的碱基序列或其变异序列组成的寡核苷酸。