公开(公告)号：US20050009072A1

公开(公告)日：2005-01-13

申请号：US10854327

申请日：2004-05-27

申请人： Koshi Maeda ,  Shinichi Fukuzono ,  Takayuki Kanda ,  Kokichi Sugano

发明人： Koshi Maeda ,  Shinichi Fukuzono ,  Takayuki Kanda ,  Kokichi Sugano

IPC分类号： G01N33/574 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68

CPC分类号： C07H21/04 ,  C12Q1/6886 ,  C12Q2600/156

摘要： A deletion in the end region of the long arm of a Chromosome 9 is efficiently detected. A genetic testing kit of bladder cancer according to the present invention includes a primer allowing for efficient amplification of a region containing a site of genetic polymorphism present in the ABO blood group genes of Chromosome 9. In the site of genetic polymorphism present in the ABO blood group genes, the frequency of heterozygote (heterozygosity) in the population is extremely high. Therefore, by detecting LOH using a polymorphic site present in the ABO blood group genes, it is possible to reliably detect a deletion near the polymorphic site, in other words, a deletion near the end of the long arm of Chromosome 9.

摘要翻译： 有效地检测染色体9的长臂的末端区域的缺失。 根据本发明的膀胱癌的遗传检测试剂盒包括允许有效扩增含有染色体9的ABO血型基因中存在的遗传多态性位点的区域的引物。在存在于ABO血液中的遗传多态性位点 群体基因，杂种（杂合性）在群体中的频率极高。 因此，通过使用存在于ABO血型基因中的多态性位点检测LOH，可以可靠地检测多态性位点附近的缺失，换句话说，在染色体9的长臂末端附近的缺失。

公开(公告)号：US07585628B2

公开(公告)日：2009-09-08

申请号：US11227233

申请日：2005-09-16

申请人： Koshi Maeda ,  Shinichi Fukuzono ,  Kokichi Sugano

发明人： Koshi Maeda ,  Shinichi Fukuzono ,  Kokichi Sugano

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q2600/158 ,  C12Q2565/501

摘要： There is provided a method of detecting or analyzing nucleic acid with a high reliability and reproducibility, capable of sampling target nucleic acid from initial target nucleic acid having not less than two types of sequences different from each other in a ratio which is proportional to that of initial target nucleic acid. A method of detecting or analyzing nucleic acid by sampling target nucleic acid from not less than two types of target nucleic acid samples having base sequences different in at least one base, and subjecting the sampled nucleic acid sample to detection or analysis; comprising steps of: obtaining the number of copies of initial target nucleic acid from the concentration of a nucleic acid in an initial target nucleic acid sample; and sampling the target nucleic acid of a predetermined number or more of copies from the initial target nucleic acid sample.

摘要翻译： 提供了一种以高可靠性和重现性检测或分析核酸的方法，其能够从具有不同于两种类型的序列的初始靶核酸中以与 初始靶核酸。 通过从具有至少一个碱基不同的碱基序列的至少两种靶核酸样品中取样靶核酸，并对取样的核酸样品进行检测或分析来检测或分析核酸的方法; 包括以下步骤：从初始靶核酸样品中核酸的浓度获得初始靶核酸的拷贝数; 并从初始靶核酸样品中取样预定数目或更多个拷贝的靶核酸。

公开(公告)号：US20060073503A1

公开(公告)日：2006-04-06

申请号：US11227233

申请日：2005-09-16

申请人： Koshi Maeda ,  Shinichi Fukuzono ,  Kokichi Sugano

发明人： Koshi Maeda ,  Shinichi Fukuzono ,  Kokichi Sugano

IPC分类号： C12Q1/68 ,  G06F19/00 ,  C12M1/34

CPC分类号： C12Q1/686 ,  C12Q2600/158 ,  C12Q2565/501

摘要： There is provided a method of detecting or analyzing nucleic acid with a high reliability and reproducibility, capable of sampling target nucleic acid from initial target nucleic acid having not less than two types of sequences different from each other in a ratio which is proportional to that of initial target nucleic acid. A method of detecting or analyzing nucleic acid by sampling target nucleic acid from not less than two types of target nucleic acid samples having base sequences different in at least one base, and subjecting the sampled nucleic acid sample to detection or analysis; comprising steps of: obtaining the number of copies of initial target nucleic acid from the concentration of a nucleic acid in an initial target nucleic acid sample; and sampling the target nucleic acid of a predetermined number or more of copies from the initial target nucleic acid sample.

公开(公告)号：US07592137B2

公开(公告)日：2009-09-22

申请号：US10854327

申请日：2004-05-27

申请人： Koshi Maeda ,  Shinichi Fukuzono ,  Takayuki Kanda ,  Kokichi Sugano

发明人： Koshi Maeda ,  Shinichi Fukuzono ,  Takayuki Kanda ,  Kokichi Sugano

IPC分类号： C07H21/04 ,  C12Q1/68

CPC分类号： C07H21/04 ,  C12Q1/6886 ,  C12Q2600/156

摘要： A deletion in the end region of the long arm of a Chromosome 9 is efficiently detected.A genetic testing kit of bladder cancer according to the present invention includes a primer allowing for efficient amplification of a region containing a site of genetic polymorphism present in the ABO blood group gene of Chromosome 9. In the site of genetic polymorphism present in the ABO blood group gene, the frequency of heterozygote (heterozygosity) in the population is extremely high. Therefore, by detecting LOH using a polymorphic site present in the ABO blood group gene, it is possible to reliably detect a deletion near the polymorphic site, in other words, a deletion near the end of the long arm of Chromosome 9.

摘要翻译： 有效地检测染色体9的长臂的末端区域的缺失。 根据本发明的膀胱癌的遗传检测试剂盒包括允许有效扩增含有染色体9的ABO血型基因中存在的遗传多态性位点的区域的引物。在存在于ABO血液中的遗传多态性位点 群体基因，种群中杂合子（杂合性）的频率极高。 因此，通过使用ABO血型基因中存在的多态性位点检测LOH，可以可靠地检测多态性位点附近的缺失，换句话说，在染色体9的长臂末端附近的缺失。