公开(公告)号：US20050084969A1

公开(公告)日：2005-04-21

申请号：US10497015

申请日：2002-11-26

Applicant: Kurt Schorgendorfer ,  Jorg Windisch ,  Renate Kunert ,  Florian Unterluggauer

Inventor： Kurt Schorgendorfer ,  Jorg Windisch ,  Renate Kunert ,  Florian Unterluggauer

IPC: C12N15/09 ,  A61K38/22 ,  A61P7/00 ,  A61P7/06 ,  C07K14/505 ,  C12N5/10 ,  C12N15/85 ,  C12N15/90 ,  C12P21/02 ,  C12N5/06

CPC classification number: C07K14/505 ,  A61K2121/00 ,  C12N15/85 ,  C12N15/90 ,  C12N15/907 ,  C12N2840/203 ,  C12P21/02

Abstract: The invention relates to a method for producing a transformed eukaryotic host cell which expresses a recombinant polypeptide of interest which method comprises introducing into a eukaryotic host cell: (a) a first polynucleotide vector which comprises (i) a first nucleotide sequence which encodes a recombinant polypeptide of interest, and (ii) a second nucleotide sequence encoding a selectable marker, which second nucleotide sequence is amplified when the host cell is contacted with a selection agent; and (b) a second polynucleotide vector having essentially the same nucleotide sequence as the first polynucleotide vector except that the second nucleotide sequence is replaced with a third nucleotide sequence which encodes a different selectable marker; the first polynucleotide vector and second polynucleotide vector being stably integrated into the genome of the host cell.

Abstract translation: 本发明涉及用于产生表达重组感兴趣的多肽的转化真核宿主细胞的方法，所述方法包括将真核宿主细胞导入：（a）第一多核苷酸载体，其包含（i）第一核苷酸序列，其编码重组 多肽，和（ii）编码选择性标记的第二核苷酸序列，当宿主细胞与选择剂接触时，第二核苷酸序列被扩增; 和（b）第二多核苷酸载体，其具有与第一多核苷酸载体基本上相同的核苷酸序列，除了第二核苷酸序列被编码不同选择标记的第三核苷酸序列替代; 第一多核苷酸载体和第二多核苷酸载体稳定整合到宿主细胞的基因组中。