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公开(公告)号：US09388376B2
公开(公告)日：2016-07-12
申请号：US14023575
申请日：2013-09-11
Applicant: Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
Inventor： Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
Abstract: A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.
Abstract translation: 一种用于计数设置在容纳在容器中的液体中的计数目标的方法，包括调节容器的至少一部分的厚度; 将所述经调整部分的至少一部分设定为测量对象区域; 并计数测量目标区域中的多个计数目标。
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公开(公告)号：US20130164831A1
公开(公告)日：2013-06-27
申请号：US13818862
申请日：2011-09-05
Applicant: Satoshi Tanaka , Takahiko Totani , Yoichi Ishizaki , Kyohei Ota , Ryo Suenaga , Masahiro Kuninori
Inventor： Satoshi Tanaka , Takahiko Totani , Yoichi Ishizaki , Kyohei Ota , Ryo Suenaga , Masahiro Kuninori
IPC: C12M1/04
CPC classification number: C12M23/24 , B32B1/02 , B32B1/08 , B32B7/02 , B32B27/08 , B32B27/32 , B32B2250/02 , B32B2250/03 , B32B2250/242 , B32B2307/31 , B32B2307/412 , B32B2307/72 , B32B2307/724 , B32B2307/732 , B32B2323/046 , B32B2439/40 , C12M23/14 , C12M23/20 , Y10T428/2495 , Y10T428/24992
Abstract: Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same.A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture surface. A cell culture container is formed by using this multilayer film.
Abstract translation: 提供了具有优异的透气性和优异的处理性能的多层膜，因此适合于形成细胞培养容器和使用该多孔膜形成的细胞培养容器。 一种用于形成细胞培养容器的多层膜，包括：由密度为0.87g / cm 3至0.90g / cm 3的聚乙烯类树脂构成的基材; 以及由密度为0.896g / cm 3至0.93g / cm 3的聚乙烯类树脂构成的内层，形成细胞培养面。 通过使用该多层膜形成细胞培养容器。
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3.发明申请CELL CULTURE METHOD, CELL CULTURE DEVICE, METHOD FOR COUNTING SUBSJECT MATTERS TO BE COUNTED IN CONTAINER AND DEVICE FOR COUNTING 审中-公开细胞培养方法，细胞培养装置，用于计数在集装箱中计数的备份物和用于计数的装置的方法公开(公告)号：US20110318725A1
公开(公告)日：2011-12-29
申请号：US13138589
申请日：2010-03-02
Applicant: Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
Inventor： Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
Abstract: Cell aggregate formation control and cell aggregate disintegration control are carried out with respect to cells in a culture container to increase cell proliferation efficiency. The number of the cells in the culture container is counted without disassembly of a culture system and irrespective of the density of the cells.An external force is applied to the culture container to carry out at least one of cell aggregate formation control and cell aggregate disintegration control with respect to cells in the culture container, thus culturing the cells. The external force is applied to the culture container by pressing an agitating member onto a top surface of the culture container placed in flat orientation to a predetermined pressing degree, and moving the agitating member in a horizontal direction at a predetermined speed, thus controlling at least one of cell aggregate formation and cell aggregate disintegration with respect to the cells in the culture container. A method for counting counted targets disposed in a liquid enclosed in a container includes adjusting a thickness of at least a part of the container. At least a part of the adjusted part is set as a measurement target region. A number of counted targets in the measurement target region is counted.
Abstract translation: 对培养容器中的细胞进行细胞聚集体形成控制和细胞聚集体分解控制，以提高细胞增殖效率。 在不拆卸培养系统的情况下对培养容器中的细胞数进行计数，而不考虑细胞的密度。 对培养容器施加外力，对培养容器中的细胞进行细胞聚集体形成控制和细胞聚集体分解控制中的至少一种，从而培养细胞。 通过将搅拌构件按照预定的压制程度将培养容器的上表面压入培养容器的上表面并以预定的速度沿水平方向移动搅拌构件，将外力施加到培养容器上，从而至少控制 相对于培养容器中的细胞的细胞聚集体形成和细胞聚集体分解中的一种。 用于计数设置在容纳在容器中的液体中的计数目标的方法包括调节至少一部分容器的厚度。 调整后的部分的至少一部分被设定为测量对象区域。 对测量目标区域中的多个计数目标进行计数。
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公开(公告)号：US20140011186A1
公开(公告)日：2014-01-09
申请号：US14023575
申请日：2013-09-11
Applicant: Ryo SUENAGA , Yoichi ISHIZAKI , Satoshi TANAKA , Takahiko TOTANI , Kyohei OTA
Inventor： Ryo SUENAGA , Yoichi ISHIZAKI , Satoshi TANAKA , Takahiko TOTANI , Kyohei OTA
IPC: C12M1/34
Abstract: A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.
Abstract translation: 一种用于计数设置在容纳在容器中的液体中的计数目标的方法，包括调节容器的至少一部分的厚度; 将所述经调整部分的至少一部分设定为测量对象区域; 并计数测量目标区域中的多个计数目标。
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