公开(公告)号：US20110060228A1

公开(公告)日：2011-03-10

申请号：US12836417

申请日：2010-07-14

申请人： Shinji Koshino ,  Hiroki Shimizu ,  Kyoko Shimizu ,  Hiroo Sato

发明人： Shinji Koshino ,  Hiroki Shimizu ,  Kyoko Shimizu ,  Hiroo Sato

IPC分类号： A61B5/026 ,  A61B6/00

CPC分类号： A61B5/0261 ,  A61B5/02028 ,  A61B5/02416 ,  A61B5/6826 ,  A61B5/6838

摘要： In order to supply a method and a device that can evaluate the fluidity of blood with good sensitivity in a simple manner, there is provided a blood fluidity evaluation method in which a pressure member is pressed against a test site upon a test subject, blood at the test site is squeezed to flow out to the periphery of the test site, change over time of the amount of blood at the test site is measured at this time using optical scattering, and blood fluidity at the test site is evaluated from this measurement data; and, desirably, before the above method, a positioning process is performed in which: the pressure member is pressed against the test site with a weak force; pulse measurement data is acquired due to the absorption of light; and a relative position, at which the intensity of the pulse measurement data attains a local maximum, is determined as being an optimum measurement position.

摘要翻译： 为了提供能够以简单的方式以良好的灵敏度评估血液的流动性的方法和装置，提供了一种血液流动性评价方法，其中将压力构件压在测试对象的测试位置，血液在 测试部位被挤压流出到测试部位的周围，此时使用光学散射测量在测试部位的血液量的变化，并且从该测量数据评估测试部位的血液流动性 ; 并且期望地在上述方法之前，执行定位处理，其中：压力构件以弱的力压在测试地点上; 由于吸收光而获取脉冲测量数据; 以及脉冲测量数据的强度达到局部最大值的相对位置被确定为最佳测量位置。

公开(公告)号：US20090176306A1

公开(公告)日：2009-07-09

申请号：US11916701

申请日：2006-06-12

申请人： Toshio Taira ,  Kyoko Shimizu ,  Masato Sakai

发明人： Toshio Taira ,  Kyoko Shimizu ,  Masato Sakai

IPC分类号： C12N5/06

CPC分类号： C12N5/0653 ,  C12N2501/91

摘要： The invention provides a method of inducing differentiation of a visceral preadipocyte and a method of culturing a visceral adipocyte comprising culturing the cell in a medium containing serum free of heparin-adsorbable components. The invention also provides for the removal of heparin-adsorbable components by, for example, applying serum to a heparin affinity column thereby adsorbing the heparin-adsorbable component to heparin. A visceral adipocyte cultured by the method of the present invention is useful in a study of differentiation, growth and metabolism of an adipocyte, elucidation of the mechanism of development and progress of diseases such as diabetes, hyperlipemia, hypertension and arteriosclerosis, and development of a drug for preventing and treating such a disease.

摘要翻译： 本发明提供诱导内脏前脂肪细胞分化的方法和培养内脏脂肪细胞的方法，包括在含有不含肝素可吸附成分的血清的培养基中培养细胞。 本发明还提供了通过例如将血清施加到肝素亲和柱上从而将肝素可吸附组分吸附到肝素中来除去肝素可吸附组分。 通过本发明的方法培养的内脏脂肪细胞可用于脂肪细胞的分化，生长和代谢的研究，阐明发育机制和疾病如糖尿病，高脂血症，高血压和动脉硬化以及发展中的发展 用于预防和治疗这种疾病的药物。

公开(公告)号：US20090233326A1

公开(公告)日：2009-09-17

申请号：US12298401

申请日：2007-04-23

申请人： Jin-ichi Inokuchi ,  Takashige Sato ,  Toshio Taira ,  Kyoko Shimizu

发明人： Jin-ichi Inokuchi ,  Takashige Sato ,  Toshio Taira ,  Kyoko Shimizu

IPC分类号： C12Q1/02 ,  C12N5/06

CPC分类号： G01N33/5044 ,  C12N5/0653 ,  C12N2501/105 ,  C12N2501/33 ,  G01N2800/042

摘要： A culture medium is disclosed for inducing the differentiation of visceral preadipocytes into mature visceral adipocytes; the culture medium contains 0.85 to 100 ng/mL insulin and 50 to 250 ng/mL IGF-1. Also disclosed is a method for using the culture medium to induce the differentiation of visceral preadipocytes into mature visceral adipocytes. Use of the differentiation induction system of the present invention enables a substantial induction of adipocyte differentiation without the addition of synthetic differentiation inducers or high insulin concentrations. The mature adipocytes obtained by the differentiation induction system of the present invention are useful for research into the biochemistry and physiology of adipocytes, for screening drugs effective for the treatment of lifestyle-related diseases such as obesity and type 2 diabetes, and for developing diagnostic reagents.

摘要翻译： 公开了一种用于诱导内脏前脂肪细胞分化为成熟内脏脂肪细胞的培养基; 培养基含有0.85至100ng / mL胰岛素和50至250ng / mL IGF-1。 还公开了使用培养基诱导内脏前脂肪细胞分化为成熟内脏脂肪细胞的方法。 使用本发明的分化诱导系统能够在不添加合成分化诱导剂或高胰岛素浓度的情况下实质上诱导脂肪细胞分化。 通过本发明的分化诱导系统获得的成熟脂肪细胞可用于研究脂肪细胞的生物化学和生理学，用于筛选有效治疗生活方式相关疾病如肥胖症和2型糖尿病的药物，以及用于开发诊断试剂 。