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    ENGINEERING THE PATHWAY FOR SUCCINATE PRODUCTION
    1.
    发明申请
    ENGINEERING THE PATHWAY FOR SUCCINATE PRODUCTION 审中-公开
    工程生产的途径

    公开(公告)号：US20120058530A1

    公开(公告)日：2012-03-08

    申请号：US13256460

    申请日：2010-04-02

    申请人： Xueli Zhang ,  Kaemwich Jantama ,  Jonathan C. Moore ,  Laura R. Jarboe ,  Keelnatham T. Shanmugam ,  Lonnie O'Neal Ingram

    发明人： Xueli Zhang ,  Kaemwich Jantama ,  Jonathan C. Moore ,  Laura R. Jarboe ,  Keelnatham T. Shanmugam ,  Lonnie O'Neal Ingram

    IPC分类号： C12P7/46 ,  C12N1/21

    CPC分类号： C12P7/46 ,  C12N9/88 ,  C12N9/93 ,  C12Y401/01 ,  C12Y604/01001

    摘要： This invention relates to the biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulations and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yield in mineral salts media during simple pH-controlled, batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with the energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenol pyruvate carboxykinase (pck) depressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such as Actinobacillus succinogens and Mannheimia succiniproducens with one difference that the biocatalysts are able to achieve this high level of succinic acid production in a minimal salt medium with carbohydrate source as opposed to the requirement for a rich media for succinic acid production by rumen bacteria.

    摘要翻译： 本发明涉及用于从可再生生物原料有效生产琥珀酸和/或其它产品的生物催化剂。 由于遗传操作和代谢进化，生物催化剂对于来自碳水化合物原料的琥珀酸和/或其它产物的生长偶联生产具有非常高的效率。 更具体地，本发明的某些生物催化剂在简单的pH控制的间歇式发酵过程中以高滴度产生琥珀酸，并在矿物盐介质中产生，而不添加任何外源遗传物质。 本发明的遗传操作涉及除琥珀酸生产途径之外的NADH氧化替代途径的节能策略。 生物催化剂含有通过遗传修饰和基因失活的磷酸转移酶系统抑制的葡萄糖抑制的糖原磷酸烯醇丙酮酸羧激酶（pck）。 在琥珀酸生产效率方面，本发明的生物催化剂在功能上等同于琥珀酸盐生产的瘤胃细菌如琥珀酸放线杆菌素和人造芽孢杆菌，其生物催化剂能够在最小的盐中达到高水平的琥珀酸生产 具有碳水化合物来源的培养基，而不是富含培养基对瘤胃细菌产生琥珀酸的要求。