公开(公告)号：US06329157B1

公开(公告)日：2001-12-11

申请号：US09086503

申请日：1998-05-28

Applicant: Gregory T. Maine ,  Jeffrey C. Hunt ,  Susan Brojanac ,  Michael Jyh-Tsing Sheu ,  Linda E. Chovan ,  Joan D. Tyner ,  Lawrence V. Howard

Inventor： Gregory T. Maine ,  Jeffrey C. Hunt ,  Susan Brojanac ,  Michael Jyh-Tsing Sheu ,  Linda E. Chovan ,  Joan D. Tyner ,  Lawrence V. Howard

IPC: G01N3353

CPC classification number: G01N33/56905 ,  Y02A50/57 ,  Y02A50/58

Abstract: The present invention relates to combinations or mixtures of antigens which may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using these combinations of antigens, antibodies raised against these combinations of antigens or against the novel P29 antigen thereof, as well as kits and vaccines containing the antigens present in the combinations.

Abstract translation: 本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的抗原的组合或混合物。 此外，本发明还涉及使用这些抗原组合，针对这些抗原组合产生的抗体或其新型P29抗原以及含有组合中存在的抗原的试剂盒和疫苗的方法。

公开(公告)号：US06294342B1

公开(公告)日：2001-09-25

申请号：US09409045

申请日：1999-09-29

Applicant: Thomas E. Rohr ,  Tuan A. Elstrom ,  Lawrence V. Howard ,  Eric B. Shain

Inventor： Thomas E. Rohr ,  Tuan A. Elstrom ,  Lawrence V. Howard ,  Eric B. Shain

IPC: G01N3353

CPC classification number: G01N33/54333 ,  G01N35/0098 ,  Y10S436/806 ,  Y10S977/958

Abstract: Assay methods utilizing the response of a magnetically responsive reagent to the influence of a magnetic field to qualitatively or quantitatively measure binding between specific binding pair members. According to the invention, the presence of an analyte mediates whether or not the magnetically responsive reagent binds to a mobile solid phase reagent. The extent of binding will modulate the response of the magnetically responsive reagent or that of the mobile solid phase reagent, or both, to the influence of a magnetic field. Hence, by measuring the response to the magnetic field of the magnetically responsive reagent, or that of the mobile solid phase reagent, the presence or amount of analyte contained in a test sample can accurately be determined. The invention utilizes various devices to carry out the assay methods described.

Abstract translation: 使用磁响应试剂对磁场影响的响应的测定方法，以定性或定量测量特异性结合对成员之间的结合。 根据本发明，分析物的存在介导了磁性响应试剂是否结合流动的固相试剂。 结合的程度将调节磁响应试剂或可移动固相试剂或两者的响应对磁场的影响。 因此，通过测量对磁响应试剂或可移动固相试剂的磁场的响应，可以准确地确定测试样品中包含的分析物的存在或量。 本发明利用各种装置来实施所述的测定方法。

公开(公告)号：US06221619B1

公开(公告)日：2001-04-24

申请号：US09303064

申请日：1999-04-30

Applicant: Gregory T. Maine ,  Jeffrey C. Hunt ,  Susan Brojanac ,  Michael Jyh-Tsing Sheu ,  Linda E. Chovan ,  Joan D. Tyner ,  Lawrence V. Howard ,  Stephen F. Parmley ,  Jack S. Remington ,  Fausto Araujo ,  Yashuhiro Suzuki ,  Shuli Li

Inventor： Gregory T. Maine ,  Jeffrey C. Hunt ,  Susan Brojanac ,  Michael Jyh-Tsing Sheu ,  Linda E. Chovan ,  Joan D. Tyner ,  Lawrence V. Howard ,  Stephen F. Parmley ,  Jack S. Remington ,  Fausto Araujo ,  Yashuhiro Suzuki ,  Shuli Li

IPC: G01N3353

CPC classification number: G01N33/56905 ,  Y02A50/57 ,  Y02A50/58

Abstract: The present invention relates to combinations or mixtures of antigens which may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii as well as to the P35 antigen which may be used to distinguish acute from chronic toxoplasmosis. Furthermore, the present invention also relates to methods of using these combinations of antigens, antibodies raised against these combinations of antigens or against the novel P29 antigen thereof, as well as kits and vaccines containing the antigens present in the combinations.

Abstract translation: 本发明涉及可用于检测弓形体的IgM和/或IgG抗体以及可用于区分急性和慢性弓形虫病的P35抗原的抗原的组合或混合物。 此外，本发明还涉及使用这些抗原组合，针对这些抗原组合产生的抗体或其新型P29抗原以及含有组合中存在的抗原的试剂盒和疫苗的方法。

公开(公告)号：US5998224A

公开(公告)日：1999-12-07

申请号：US857440

申请日：1997-05-16

Applicant: Thomas E. Rohr ,  Tuan A. Elstrom ,  Lawrence V. Howard ,  Eric B. Shain

Inventor： Thomas E. Rohr ,  Tuan A. Elstrom ,  Lawrence V. Howard ,  Eric B. Shain

IPC: G01N33/553 ,  G01N33/543

CPC classification number: G01N33/54333 ,  G01N33/54366 ,  Y10S436/806 ,  Y10S977/773 ,  Y10S977/838 ,  Y10S977/89 ,  Y10S977/92 ,  Y10S977/96

Abstract: Assay methods utilizing the response of a magnetically responsive reagent to the influence of a magnetic field to qualitatively or quantitatively measure binding between specific binding pair members. According to the invention, the presence of an analyte mediates whether or not the magnetically responsive reagent binds to a mobile solid phase reagent. The extent of binding will modulate the response of the magnetically responsive reagent or that of the mobile solid phase reagent, or both, to the influence of a magnetic field. Hence, by measuring the response to the magnetic field of the magnetically responsive reagent, or that of the mobile solid phase reagent, the presence or amount of analyte contained in a test sample can accurately be determined. The invention utilizes various devices to carry out the assay methods described.

Abstract translation: 使用磁响应试剂对磁场影响的响应的测定方法，以定性或定量测量特异性结合对成员之间的结合。 根据本发明，分析物的存在介导了磁性响应试剂是否结合流动的固相试剂。 结合的程度将调节磁响应试剂或可移动固相试剂或两者的响应对磁场的影响。 因此，通过测量对磁响应试剂或可移动固相试剂的磁场的响应，可以准确地确定测试样品中包含的分析物的存在或量。 本发明利用各种装置来实施所述的测定方法。

公开(公告)号：US4497899A

公开(公告)日：1985-02-05

申请号：US479414

申请日：1983-03-28

Applicant: Alan S. Armstrong ,  John E. Herrmann ,  Lawrence V. Howard

Inventor： Alan S. Armstrong ,  John E. Herrmann ,  Lawrence V. Howard

IPC: G01N33/569 ,  G01N33/54 ,  G01N33/56

CPC classification number: G01N33/56927 ,  Y10S435/962 ,  Y10S436/804 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S436/823 ,  Y10S436/825 ,  Y10S436/826

Abstract: The present disclosure relates to a solid phase immunoassay for the detection of Chlamydia trachomatis antigens in a clinical specimen, wherein the Chlamydia trachomatis antigens to be determined are coated or adsorbed on the solid phase.

Abstract translation: 本公开涉及用于在临床标本中检测沙眼衣原体抗原的固相免疫测定法，其中待测定的衣原体沙眼衣原体抗原被包被或吸附在固相上。

公开(公告)号：US5064755A

公开(公告)日：1991-11-12

申请号：US228363

申请日：1988-08-04

Applicant: Lawrence V. Howard, Jr. ,  MaryCaren Craine

Inventor： Lawrence V. Howard, Jr. ,  MaryCaren Craine

IPC: G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/569

CPC classification number: G01N33/542 ,  G01N33/56927

Abstract: In an assay where a ligand or organism is detected by a detector binding protein ("DBP") capable of generating a signal in proportion to the amount of ligand or organism bound to the DBP, the presence of the ligand or organism is confirmed by the use, prior to or concurrently with the DBP, of a confirmatory binding protein ("CBP") which binds to a second site on the ligand or organism, and thereby prevents the DBP from binding to the first site. Thus, a reduction in signal of a predetermined amount indicates the true presence of the ligand or organism, while failure to obtain signal reduction of a predetermined amount indicates that the original signal was a false positive due to assay artifact or detection of related ligands or organisms. In a preferred embodiment, the CBP is a monoclonal antibody and the organism is derived from Chlamydia species.