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    Solid phase radioimmunoassay of digoxin
    2.
    发明授权
    Solid phase radioimmunoassay of digoxin 失效
    地高辛的固相放射免疫测定

    公开(公告)号：US3933997A

    公开(公告)日：1976-01-20

    申请号：US447251

    申请日：1974-03-01

    申请人： Leroy S. Hersh ,  Sidney Yaverbaum

    发明人： Leroy S. Hersh ,  Sidney Yaverbaum

    IPC分类号： G01N33/53 ,  G01N31/16 ,  G01N33/543 ,  G01N33/553 ,  A61K43/00 ,  G01T1/16 ,  G01T1/167 ,  G21H5/02

    CPC分类号： G01N33/54326 ,  G01N31/16 ,  Y10S436/804 ,  Y10S436/815 ,  Y10S436/817

    摘要： Clinically significant concentrations of digoxin can be determined by reacting an unknown amount of digoxin, a known amount of labelled digoxin, and a composite consisting of anti-digoxin antibodies coupled through an intermediate silane to magnetically responsive particles; magnetically separating the immunochemical complexes formed thereby, counting the radioactivity of either the separated products or remaining solution, and relating the count to a standard curve.

    摘要翻译： 可以通过使未知量的地高辛（已知量的标记的地高辛）和由通过中间体硅烷偶联的抗地高辛抗体与磁性响应性颗粒组成的复合物反应来确定临床显着浓度的地高辛浓度; 磁分离由此形成的免疫化学复合物，计数分离的产物或剩余溶液的放射性，并将计数与标准曲线相关联。

    Oxidized styrenic polymers for DNA binding
    3.
    发明授权
    Oxidized styrenic polymers for DNA binding 失效
    用于DNA结合的氧化苯乙烯聚合物

    公开(公告)号：US06391655B1

    公开(公告)日：2002-05-21

    申请号：US09124352

    申请日：1998-07-29

    申请人： Dana C. Bookbinder ,  Leroy S. Hersh ,  Xinying Xie

    发明人： Dana C. Bookbinder ,  Leroy S. Hersh ,  Xinying Xie

    IPC分类号： G01N33545

    CPC分类号： C07H21/00 ,  C12Q1/6834 ,  G01N33/54393

    摘要： A method of immobilizing aminated DNA to a substrate surface is provided for use in DNA hybridization assays. The surface can be widely employed in a variety of molded laboratory products including multiwell plates, cell culture dishes, and biological containers. The method comprises the steps of providing a polystyrene substrate having a surface content of between 0.3 and 4.0% atomic percent oxygen, and attaching amine modified oligonucleotide to the surface of the substrate. A resultant product comprising a polystyrene substrate having a surface oxygen content of between 0.3 and 4.0% atomic percent, and a plurality of amine modified oligonucleotides directly attached to the surface of the substrate, is also disclosed.

    摘要翻译： 提供将胺化的DNA固定到底物表面的方法用于DNA杂交测定。 该表面可广泛用于各种模制实验室产品，包括多孔板，细胞培养皿和生物容器。 该方法包括以下步骤：提供具有0.3至4.0％原子百分比氧的表面含量的聚苯乙烯底物，并将胺修饰的寡核苷酸连接到基底的表面。 还公开了一种包含表面氧含量为0.3-4.0％原子百分比的聚苯乙烯底物和直接附着在基材表面上的多个胺修饰的寡核苷酸的产物。