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公开(公告)号：US07537771B2
公开(公告)日：2009-05-26
申请号：US10332282
申请日：2001-07-06
申请人： Ethel Diane Williamson , Julie Miller , Nicola Jane Walker , Leslie William James Baillie , Paula Thomson Holden , Helen Claire Flick-Smith , Helen Lisa Bullifent , Richard William Titball , Andrew William Topping
发明人： Ethel Diane Williamson , Julie Miller , Nicola Jane Walker , Leslie William James Baillie , Paula Thomson Holden , Helen Claire Flick-Smith , Helen Lisa Bullifent , Richard William Titball , Andrew William Topping
CPC分类号： C07K14/32 , A61K39/00 , C07K2319/00 , C12N15/70
摘要： An immunogenic reagent which produces an immune response which is protective against Bacillus anthracis, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from E. coli. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.
摘要翻译： 产生免疫应答的免疫原性试剂，其为防止炭疽芽孢杆菌（Bacillus anthracis）提供免疫应答，所述试剂包含一个或多个多个共同代表炭疽芽孢杆菌或其变体的全长保护性抗原（PA）的三个结构域的多肽，至少 所述结构域之一包括PA的结构域1或结构域4或其变体。 免疫原性试剂的多肽以及全长PA通过来自大肠杆菌的表达产生。 使用该方法获得高产量的多肽。 还描述和要求保护该方法中使用的细胞，载体和核酸。
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公开(公告)号：US08313928B2
公开(公告)日：2012-11-20
申请号：US11728384
申请日：2007-03-26
申请人： Ethel Diane Williamson , Julie Miller , Nicola Jane Walker , Leslie William James Baillie , Paula Thomson Holden , Helen Claire Flick-Smith , Helen Lisa Bullifent , Richard William Titball , Andrew William Topping
发明人： Ethel Diane Williamson , Julie Miller , Nicola Jane Walker , Leslie William James Baillie , Paula Thomson Holden , Helen Claire Flick-Smith , Helen Lisa Bullifent , Richard William Titball , Andrew William Topping
CPC分类号： C07K14/32 , A61K39/00 , C07K2319/00 , C12N15/70
摘要： An immunogenic reagent which produces an immune response which is protective against Bacillus anthracis, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from E. coli. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.
摘要翻译： 产生免疫应答的免疫原性试剂，其为防止炭疽芽孢杆菌（Bacillus anthracis）提供免疫应答，所述试剂包含一个或多个多个共同代表炭疽芽孢杆菌或其变体的全长保护性抗原（PA）的三个结构域的多肽，至少 所述结构域之一包括PA的结构域1或结构域4或其变体。 免疫原性试剂的多肽以及全长PA通过来自大肠杆菌的表达产生。 使用该方法获得高产量的多肽。 还描述和要求保护该方法中使用的细胞，载体和核酸。
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公开(公告)号：US20130164823A1
公开(公告)日：2013-06-27
申请号：US13627071
申请日：2012-09-26
申请人： Ethel Diane Williamson , Julie Miller , Nicola Jane Walker , Leslie William James Baillie , Paula Thomson Holden , Helen Claire Flick-Smith , Helen Lisa Bullifent , Richard William Titball , Andrew William Topping
发明人： Ethel Diane Williamson , Julie Miller , Nicola Jane Walker , Leslie William James Baillie , Paula Thomson Holden , Helen Claire Flick-Smith , Helen Lisa Bullifent , Richard William Titball , Andrew William Topping
IPC分类号： C12N15/70
CPC分类号： C07K14/32 , A61K39/00 , C07K2319/00 , C12N15/70
摘要： An immunogenic reagent which produces an immune response which is protective against Bacillus anthracis, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA ro a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from E. coli. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.
摘要翻译： 产生免疫应答的免疫原性试剂，其为防止炭疽芽孢杆菌（Bacillus anthracis）提供免疫应答，所述试剂包含一个或多个多个共同代表炭疽芽孢杆菌或其变体的全长保护性抗原（PA）的三个结构域的多肽，至少 所述结构域之一包括其变体PA的结构域1或结构域4。 免疫原性试剂的多肽以及全长PA通过来自大肠杆菌的表达产生。 使用该方法获得高产量的多肽。 还描述和要求保护该方法中使用的细胞，载体和核酸。
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公开(公告)号：US20100266639A1
公开(公告)日：2010-10-21
申请号：US11728384
申请日：2007-03-26
申请人： Ethel Diane Williamson , Julie Miller , Nicola Jane Walker , Leslie William James Baillie , Paula Thomson Holden , Helen Claire Flick-Smith , Helen Lisa Bullifent , Richard William Titball , Andrew William Topping
发明人： Ethel Diane Williamson , Julie Miller , Nicola Jane Walker , Leslie William James Baillie , Paula Thomson Holden , Helen Claire Flick-Smith , Helen Lisa Bullifent , Richard William Titball , Andrew William Topping
CPC分类号： C07K14/32 , A61K39/00 , C07K2319/00 , C12N15/70
摘要： An immunogenic reagent which produces an immune response which is protective against Bacillus anthracis, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from E. coli. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.
摘要翻译： 产生免疫应答的免疫原性试剂，其为防止炭疽芽孢杆菌（Bacillus anthracis）提供免疫应答，所述试剂包含一个或多个多个共同代表炭疽芽孢杆菌或其变体的全长保护性抗原（PA）的三个结构域的多肽，至少 所述结构域之一包括PA的结构域1或结构域4或其变体。 免疫原性试剂的多肽以及全长PA通过来自大肠杆菌的表达产生。 使用该方法获得高产量的多肽。 还描述和要求保护该方法中使用的细胞，载体和核酸。
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公开(公告)号：US07270991B2
公开(公告)日：2007-09-18
申请号：US10483150
申请日：2002-07-09
摘要： A recombinant microorganism comprises an asporogenic Bacillus subtilis strain in which a gene encoding a protease enzyme has been downregulated or inactivated. In particular sigma factorspoIIAC is inactivated such that the strain is asporogenic. These strains are particularly useful as expression vehicles for proteins such as protective antigen (PA) of Bacillus anthracis.
摘要翻译： 重组微生物包含其中编码蛋白酶的基因已被下调或失活的异源枯草芽孢杆菌菌株。 特别是sigma因子pIIAC被灭活，使得菌株是异源的。 这些菌株特别可用作蛋白质的表达载体，例如炭疽芽孢杆菌的保护性抗原（PA）。
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