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    Method for a fully automated monoclonal antibody-based extended differential
    1.
    发明授权
    Method for a fully automated monoclonal antibody-based extended differential 有权
    全自动单克隆抗体扩增差异的方法

    公开(公告)号：US07674598B2

    公开(公告)日：2010-03-09

    申请号：US11475313

    申请日：2006-06-27

    申请人： Ronald D. Paul ,  Oilda Rubio ,  Diana B. Careaga ,  Lidice L. Lopez ,  Ravindra Mylvaganam

    发明人： Ronald D. Paul ,  Oilda Rubio ,  Diana B. Careaga ,  Lidice L. Lopez ,  Ravindra Mylvaganam

    IPC分类号： G01N33/567

    CPC分类号： G01N33/56966 ,  G01N15/1459 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/13 ,  Y10T436/25125

    摘要： Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.

    摘要翻译： 用于差异鉴定仪器中的细胞的方法使用含有选择的抗体和具有不同细胞分布模式和特异性的荧光染料的组合的组合物，以及以形成不可补偿光谱图案的重叠发射光谱为特征的抗体和荧光染料。 当利用由本文描述的由荧光染料和荧光染料标记的抗体组成的组合物时，基于光学或电子补偿装置不能被分离或区分的重叠光谱，建立了新的荧光足迹。 这种新的荧光足迹是由组合物选择的染料和荧光染料标记的抗体的重叠光谱和组合的细胞染色模式的结果。 新的荧光足迹导致直方图模式，可用于鉴定血液疾病中的其他细胞群体或亚型。

    Method for a fully automated monoclonal antibody-based extended differential
    3.
    发明授权
    Method for a fully automated monoclonal antibody-based extended differential 有权
    全自动单克隆抗体扩增差异的方法

    公开(公告)号：US07625712B2

    公开(公告)日：2009-12-01

    申请号：US11130492

    申请日：2005-05-17

    申请人： Ronald D. Paul ,  James L. Wyatt ,  Barbara Carrillo ,  Oilda Rubio ,  Diana B. Careaga ,  Lidice L. Lopez

    发明人： Ronald D. Paul ,  James L. Wyatt ,  Barbara Carrillo ,  Oilda Rubio ,  Diana B. Careaga ,  Lidice L. Lopez

    IPC分类号： G01N33/567

    CPC分类号： G01N33/56966 ,  G01N15/1459 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/13 ,  Y10T436/25125

    摘要： A method useful for the enumeration of cell populations in a biological sample includes the steps of reacting in a single reaction mixture a sample, a first antibody labeled with a fluorochrome having a first emission spectrum and an additional antibody. The first antibody binds to an antigenic determinant differentially expressed on leukocytes and non-leukocytes. The additional antibody binds to an antigenic determinant differentially expressed on mature and immature granulocytes or myeloid cells, and is labeled either with the first fluorochrome or an additional fluorochrome having an emission spectrum distinguishable from the first emission spectrum. The reaction mixture can be mixed with a nucleic acid dye having an emission spectrum that overlaps with one of the first or additional emission spectra. The reaction mixture may be treated with a lytic system that differentially lyses non-nucleated red blood cells and conserves leukocytes. Populations of hematological cells are detected and enumerated using at least two parameters (fluorescence, optical, and electrical) for each population.

    摘要翻译： 可用于计数生物样品中细胞群体的方法包括以下步骤：在单一反应混合物中使样品，用具有第一发射光谱的荧光染料标记的第一抗体和另外的抗体反应。 第一抗体结合在白细胞和非白细胞上差异表达的抗原决定簇。 另外的抗体结合在成熟和未成熟粒细胞或骨髓细胞上差异表达的抗原决定簇，并且用第一荧光染料或具有与第一发射光谱可区分的发射光谱的附加荧光染料进行标记。 反应混合物可以与具有与第一或另外的发射光谱之一重叠的发射光谱的核酸染料混合。 反应混合物可以用裂解体系来处理，所述裂解系统差异地裂解非成核红细胞并保存白细胞。 使用每个群体的至少两个参数（荧光，光学和电）检测和计数血细胞的群体。

    Dye compositions which provide enhanced differential fluorescence and light scatter characteristics
    4.
    发明授权
    Dye compositions which provide enhanced differential fluorescence and light scatter characteristics 失效
    提供增强的差分荧光和光散射特性的染料组合物

    公开(公告)号：US06955872B2

    公开(公告)日：2005-10-18

    申请号：US10392518

    申请日：2003-03-20

    申请人： John A. Maples ,  Lidice L. Lopez ,  Nancy Torke

    发明人： John A. Maples ,  Lidice L. Lopez ,  Nancy Torke

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  G01N20060101 ,  G01N1/30 ,  G01N33/58

    CPC分类号： G01N1/30 ,  C07H21/04 ,  C12Q1/6818 ,  G01N15/1459 ,  G01N33/582 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477

    摘要： A composition for enhancing differential staining of RNA, DNA and granules in a sample comprising cells contains a first fluorescent dye that can bind specific binding sites and non-specific binding sites in the sample. This first dye emits fluorescence at a first wavelength. The composition contains at least an additional component, which is a second non-intercalating dye in the composition that competes with said first dye for binding to the nonspecific binding sites, or a permeabilizing agent to enhance permabilization of the dyes into the cells, or both. The molar ratio of the second dye and the first dye is at least about 20:1.

    摘要翻译： 用于增强包含细胞的样品中RNA，DNA和颗粒的差异染色的组合物含有可结合样品中特异性结合位点和非特异性结合位点的第一荧光染料。 该第一染料以第一波长发射荧光。 组合物含有至少一种另外的组分，其是组合物中与第一种染料竞争结合非特异性结合位点的第二种非插层染料，或透性剂以增强染料进入细胞的均匀性，或两者兼有 。 第二染料和第一染料的摩尔比为至少约20:1。