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    GENE CLUSTER FOR THURINGIENSIN SYNTHESIS
    1.
    发明申请
    GENE CLUSTER FOR THURINGIENSIN SYNTHESIS 审中-公开
    神经元合成基因簇

    公开(公告)号：US20120015404A1

    公开(公告)日：2012-01-19

    申请号：US13145071

    申请日：2009-12-15

    申请人： Ming Sun ,  Ziniu Yu ,  Lifang Ruan ,  Shouwen Chen ,  Xiaoyan Liu ,  Donghai Peng

    发明人： Ming Sun ,  Ziniu Yu ,  Lifang Ruan ,  Shouwen Chen ,  Xiaoyan Liu ,  Donghai Peng

    IPC分类号： C12P19/44 ,  C12N1/20 ,  C12N15/61

    CPC分类号： C12N15/52 ,  C07K14/32

    摘要： The invention belongs to the technical field of genetic engineering of agricultural microorganisms. Specifically, the invention relates to producing and using gene cluster for thuringiensin synthesis from Bacillus thuringiensis. The gene cluster includes 11 genes of thuA, thuB, thuC, thuD, thuE, thuF, thuG, thu1, thu2, thu3 and thu4. These genes have the nucleotide sequence as shown in SEQ ID NO: 1. And the thuringiensin biosynthetase encoded by these genes have the amino acid sequences as shown in SEQ ID NO: 2-12. The genes according to the invention and the proteins encoded by those genes can be used as new nucleosides lead compound of pesticide library and provide new target for developing insecticides. In practical use, a series of new, efficient and low toxic insecticide can be obtained by structure modification.

    摘要翻译： 本发明属于农业微生物遗传工程技术领域。 具体地说，本发明涉及生产和使用苏云金芽孢杆菌的苏氨酸素合成基因簇。 基因簇包含thuA，thuB，thuC，thuD，thuE，thuF，thuG，thu1，thu2，thu3和thu4等11个基因。 这些基因具有如SEQ ID NO：1所示的核苷酸序列。由这些基因编码的苏云金素生物合成酶具有如SEQ ID NO：2-12所示的氨基酸序列。 根据本发明的基因和由这些基因编码的蛋白质可以用作农药图谱的新核苷酸铅化合物，并提供开发杀虫剂的新靶标。 在实际应用中，通过结构修饰可以获得一系列新型，高效，低毒的杀虫剂。

    Gene Cry7ba1 encoding an insecticidal crystal protein of Bacillus thuringiensis
    3.
    发明授权
    Gene Cry7ba1 encoding an insecticidal crystal protein of Bacillus thuringiensis 有权
    Gene Cry7ba1编码苏云金芽孢杆菌的杀虫晶体蛋白

    公开(公告)号：US07838293B2

    公开(公告)日：2010-11-23

    申请号：US12089992

    申请日：2006-10-17

    申请人： Ming Sun ,  Ziniu Yu ,  Shouwen Chen ,  Zhenyu Zhang ,  Lifang Ruan ,  Suxia Guo ,  Jingyuan Dai ,  Lin Li ,  Ziduo Liu

    发明人： Ming Sun ,  Ziniu Yu ,  Shouwen Chen ,  Zhenyu Zhang ,  Lifang Ruan ,  Suxia Guo ,  Jingyuan Dai ,  Lin Li ,  Ziduo Liu

    IPC分类号： C12N15/32 ,  C12N15/82 ,  C12N1/00 ,  C07K14/325

    CPC分类号： A01N63/00 ,  C07K14/325

    摘要： The present invention discloses the isolation, cloning and use of insecticidal crystal proteins from Bacillus thuringiensis. The present invention isolates a novel insecticidal crystal protein gene cry7Ba1 from B. thuringiensis subsp. huazhongensis YBT-978 strain, and said gene encodes a novel insecticidal crystal protein Cry7Ba1, which shows insecticidal activity against Lepidopteran insects. The present invention also discloses the gene sequence of the novel insecticidal crystal protein, uses suitable expression vectors to transform micro-organisms so as to express the product Cry7Ba1 encoded by the gene and make it exert the insecticidal activity of the protein against Lepidopteran pests.

    摘要翻译： 本发明公开了苏云金芽孢杆菌的杀虫晶体蛋白的分离，克隆和使用。 本发明从苏云金芽孢杆菌亚种中分离出新的杀虫晶体蛋白基因cry7Ba1。 华中华YBT-978株，该基因编码一种新型杀虫晶体蛋白Cry7Ba1，对鳞翅目昆虫具有杀虫活性。 本发明还公开了新型杀虫晶体蛋白的基因序列，使用合适的表达载体转化微生物，以表达由该基因编码的产物Cry7Ba1，并使其发挥蛋白质对鳞翅目害虫的杀虫活性。

    Gene Cry7ba1 Encoding and Insecticidal Crystal Protein of Bacillus Thuringiensis
    5.
    发明申请
    Gene Cry7ba1 Encoding and Insecticidal Crystal Protein of Bacillus Thuringiensis 有权
    基因Cry7ba1编码和苏云金芽孢杆菌的杀虫晶体蛋白

    公开(公告)号：US20090130758A1

    公开(公告)日：2009-05-21

    申请号：US12089992

    申请日：2006-10-17

    申请人： Ming Sun ,  Ziniu Yu ,  Shouwen Chen ,  Zhenyu Zhang ,  Lifang Ruan ,  Suxia Guo ,  Jingyuan Dai ,  Lin Li ,  Ziduo Liu

    发明人： Ming Sun ,  Ziniu Yu ,  Shouwen Chen ,  Zhenyu Zhang ,  Lifang Ruan ,  Suxia Guo ,  Jingyuan Dai ,  Lin Li ,  Ziduo Liu

    IPC分类号： C12N5/10 ,  C12N15/32 ,  C12N15/82 ,  C12N15/74 ,  C12N1/21 ,  C07K14/325

    CPC分类号： A01N63/00 ,  C07K14/325

    摘要： The present invention discloses the isolation, cloning and use of insecticidal crystal proteins from Bacillus thuringiensis. The present invention isolates a novel insecticidal crystal protein gene cry7Bal from B. thuringiensis subsp. huazhongensis YBT-978 strain, and said gene encodes a novel insecticidal crystal protein Cry7Bal, which shows insecticidal activity against Lepidopteran insects. The present invention also discloses the gene sequence of the novel insecticidal crystal protein, uses suitable expression vectors to transform micro-organisms so as to express the product Cry7Bal encoded by the gene and make it exert the insecticidal activity of the protein against Lepidopteran pests.

    摘要翻译： 本发明公开了苏云金芽孢杆菌的杀虫晶体蛋白的分离，克隆和使用。 本发明从苏云金芽孢杆菌亚种中分离出新的杀虫晶体蛋白基因cry7Bal。 华中华YBT-978株，该基因编码一种新型杀虫晶体蛋白Cry7Bal，对鳞翅目昆虫具有杀虫活性。 本发明还公开了新型杀虫晶体蛋白的基因序列，使用合适的表达载体转化微生物，以表达由该基因编码的产物Cry7Bal，并使其发挥蛋白质对鳞翅目害虫的杀虫活性。