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    Asymmetric PCR amplification, its special primer and application
    1.
    发明授权
    Asymmetric PCR amplification, its special primer and application 有权
    不对称PCR扩增，其特殊引物及应用

    公开(公告)号：US08735067B2

    公开(公告)日：2014-05-27

    申请号：US11661069

    申请日：2004-11-22

    申请人： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    发明人： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2531/107 ,  C12Q2525/155 ,  C12Q2525/15

    摘要： The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.

    摘要翻译： 本发明公开了一种不对称PCR扩增方法及其特异性引物及其应用，旨在为单链产物的制备提供简单有效的PCR扩增。 本发明的不对称PCR引物包含一些PCR引物对，其中将待检测的靶序列的不相关核酸序列添加到一个引物的5'末端。 提供的不对称PCR扩增包括以下步骤：1）制备型变性; 2）重复变性，引物退火，延伸循环作为PCR扩增的第一阶段; 3）重复变性，引物延伸循环作为PCR扩增的第二阶段，其中将待检测的靶序列的不相关核酸序列添加到每对扩增中的一个PCR引物的5'末端。 利用本发明的不对称PCR扩增，可以获得高通量的单链产物，可进行单PCR扩增或多重PCR扩增。 该方法可广泛用于检测核酸。

    Asymmetric PCR Amplification, its Special Primer and Application
    3.
    发明申请
    Asymmetric PCR Amplification, its Special Primer and Application 有权
    不对称PCR扩增，其特殊引物和应用

    公开(公告)号：US20100151448A1

    公开(公告)日：2010-06-17

    申请号：US11661069

    申请日：2004-11-22

    申请人： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    发明人： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2531/107 ,  C12Q2525/155 ,  C12Q2525/15

    摘要： The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.

    摘要翻译： 本发明公开了一种不对称PCR扩增方法及其特异性引物及其应用，旨在为单链产物的制备提供简单有效的PCR扩增。 本发明的不对称PCR引物包含一些PCR引物对，其中将待检测的靶序列的不相关核酸序列添加到一个引物的5'末端。 提供的不对称PCR扩增包括以下步骤：1）制备型变性; 2）重复变性，引物退火，延伸循环作为PCR扩增的第一阶段; 3）重复变性，引物延伸循环作为PCR扩增的第二阶段，其中将待检测的靶序列的不相关核酸序列添加到每对扩增中的一个PCR引物的5'末端。 利用本发明的不对称PCR扩增，可以获得高通量的单链产物，可进行单PCR扩增或多重PCR扩增。 该方法可广泛用于检测核酸。