公开(公告)号：US20130261196A1

公开(公告)日：2013-10-03

申请号：US13703489

申请日：2011-06-10

申请人： Lisa Diamond ,  Jochen Kumm ,  Philip Alexander Rolfe

发明人： Lisa Diamond ,  Jochen Kumm ,  Philip Alexander Rolfe

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/708 ,  C12N15/1089 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/689 ,  C12Q1/701 ,  C12Q2563/179 ,  C12Q2535/101 ,  C12Q2525/155 ,  C12Q2537/143

摘要： The invention provides mixtures of linear nucleic acid probes, including circularizing “capture” probes, capable of massively multiplex capture of one or more sequences of interest from a plurality of target organisms. The methods provided by the invention enable rapid, precise, and economical detection of one or more organisms of interest, such as common pathogens.

摘要翻译： 本发明提供线性核酸探针的混合物，其包括环化“捕获”探针，能够从多个目标生物体中大量多重捕获一种或多种感兴趣的序列。 本发明提供的方法能够快速，精确和经济地检测感兴趣的一种或多种生物，例如常见的病原体。

公开(公告)号：US07989185B2

公开(公告)日：2011-08-02

申请号：US11945960

申请日：2007-11-27

申请人： Nader Pourmand ,  Lisa Diamond ,  Jochen Kumm ,  Ronald W. Davis

发明人： Nader Pourmand ,  Lisa Diamond ,  Jochen Kumm ,  Ronald W. Davis

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/701

摘要： A rapid diagnostic assay for influenza virus, particularly avian influenza and more particularly H5N1, is described. The assay is based on amplification of a significant portion of the hemagglutinin (HA) gene and sequencing of several loci within the HA gene, using techniques which can obtain real time sequence information from multiple sites of a target DNA, in particular pyrosequencing and bioluminescence regenerative cycle. The assay contemplates the use of information-rich subsequences within the HA gene, e.g., (1) a glycosylation sequon; (2) receptor binding site; and (3) HA1/HA2 cleavage site. Other subsequences for sequencing include strain and clade markers, which vary among H5N1 strains.

摘要翻译： 描述了流感病毒，特别是禽流感，特别是H5N1的快速诊断测定。 该测定基于使用可从靶DNA的多个位点获得实时序列信息的技术，特别是焦磷酸测序和生物发光再生的技术，基于对血凝素（HA）基因的重要部分的扩增和HA基因内几个基因座的测序 周期。 该测定考虑了在HA基因内使用富含信息的子序列，例如（1）糖基化序列; （2）受体结合位点; 和（3）HA1 / HA2切割位点。 用于测序的其他子序列包括菌株和进化枝标记，其在H5N1菌株中不同。

公开(公告)号：US20050222014A1

公开(公告)日：2005-10-06

申请号：US11139730

申请日：2005-05-26

申请人： Lisa Diamond ,  Dennis Earle ,  Annette Shadiack ,  Shubh Sharma ,  Carl Spana

发明人： Lisa Diamond ,  Dennis Earle ,  Annette Shadiack ,  Shubh Sharma ,  Carl Spana

IPC分类号： A61K31/00 ,  A61K31/519 ,  A61K33/24 ,  A61K38/08 ,  A61K38/12 ,  A61K45/06 ,  A61K49/00 ,  C07K7/00 ,  C07K7/64 ,  C07K14/685

CPC分类号： C07K14/685 ,  A61K31/519 ,  A61K33/24 ,  A61K38/12 ,  A61K45/06 ,  Y10S436/811 ,  A61K2300/00

摘要： Multiple agent therapy for treatment of sexual dysfunction, including male erectile dysfunction, with sequential administration a type V phosphodiesterase inhibitor (PDE-5), such as sildenafil, preferably wherein the PDE-5 inhibitor is administered by oral dose means, and a melanocortin 3 and/or 4 receptor agonist, such as Ac-Nle-cyclo(-Asp-His-D-Phe-Arg-Trp-Lys)-OH (PT-141) preferably wherein the PT-141 is formulated for and administered by intranasal means, and further preferably wherein the PDE-5 inhibitor is administered prior to PT-141.

摘要翻译： 用于治疗性功能障碍（包括男性勃起功能障碍）的多药物治疗，连续施用V型磷酸二酯酶抑制剂（PDE-5），例如西地那非，其中PDE-5抑制剂通过口服剂量方式施用，黑皮质素3 和/或4受体激动剂，例如Ac-Nle-cyclo（-Asp-His-D-Phe-Arg-Trp-Lys）-OH（PT-141），优选其中PT-141配制用于鼻内给药 是指，进一步优选其中PDE-5抑制剂在PT-141之前施用。

公开(公告)号：US20090123909A1

公开(公告)日：2009-05-14

申请号：US11945960

申请日：2007-11-27

申请人： Nader Pourmand ,  Lisa Diamond ,  Jochen Kumm ,  Ronald W. Davis

发明人： Nader Pourmand ,  Lisa Diamond ,  Jochen Kumm ,  Ronald W. Davis

IPC分类号： C12Q1/70 ,  C12Q1/68

CPC分类号： C12Q1/701

摘要： A rapid diagnostic assay for influenza virus, particularly avian influenza and more particularly H5N1, is described. The assay is based on amplification of a significant portion of the hemagglutinin (HA) gene and sequencing of several loci within the HA gene, using techniques which can obtain real time sequence information from multiple sites of a target DNA, in particular pyrosequencing and bioluminescence regenerative cycle. The assay contemplates the use of information-rich subsequences within the HA gene, e.g., (1) a glycosylation sequon; (2) receptor binding site; and (3) HA1/HA2 cleavage site. Other subsequences for sequencing include strain and clade markers, which vary among H5N1 strains.

摘要翻译： 描述了流感病毒，特别是禽流感，特别是H5N1的快速诊断测定。 该测定基于使用可从靶DNA的多个位点获得实时序列信息的技术，特别是焦磷酸测序和生物发光再生的技术，基于对血凝素（HA）基因的重要部分的扩增和HA基因内几个基因座的测序 周期。 该测定考虑了在HA基因内使用富含信息的子序列，例如（1）糖基化序列; （2）受体结合位点; 和（3）HA1 / HA2切割位点。 用于测序的其他子序列包括菌株和进化枝标记，其在H5N1菌株中不同。

公开(公告)号：US20060107337A1

公开(公告)日：2006-05-18

申请号：US11322999

申请日：2005-12-30

申请人： Cunqi Cui ,  Lisa Diamond ,  John Logan

发明人： Cunqi Cui ,  Lisa Diamond ,  John Logan

IPC分类号： A01K67/027 ,  C12Q1/70 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/86

CPC分类号： C07K14/005 ,  A01K67/027 ,  A01K2227/108 ,  A01K2267/02 ,  C12N2740/10022 ,  C12Q1/701 ,  C12Q1/702

摘要： Porcine nucleic acid sequences flanking potentially infectious porcine endogenous retroviral (PERV) insertion sites have been identified and isolated. The unique flanking sequences include porcine nucleic acid sequences that flank the 3′ end and porcine nucleic acid sequences that flank the 5′ end of PERV insertion sites. The present invention provides compositions and methods for detecting presence of PERV in a sample, particularly those with infectious potential. In addition, the invention relates to breeding of pigs or selection of porcine tissue that is free of infectious PERV for use as a xenotransplant tissue.

公开(公告)号：US20200326325A1

公开(公告)日：2020-10-15

申请号：US16383491

申请日：2019-04-12

申请人： Lisa Diamond

发明人： Lisa Diamond

IPC分类号： G01N33/487 ,  G01N27/12

摘要： A nanosensor chip for detecting and/or quantifying target molecules in a liquid sample includes a semiconductor or other substrate and one or more electrode structures. The substrate has one or more compound nanopores, referred to as “compores.” Each compore is an aperture formed in the substrate and comprises a plurality of nanopores. Each of the nanopores is functionalized with immobilized probe molecules for detecting the target molecules. For each compore, a corresponding electrode structure is laid out on the substrate. The electrode structure has a shape and a position relative to the compore to apply an electric field across all of the nanopores in the compore and to provide a conductive path for an aggregate current through all of the nanopores in the compore. The aggregate current changes in response to target molecules in the liquid sample binding to the probe molecules as a function of the electric field.